A PCR protocol for monitoring the purity of covid19 mRNA vaccines and their recipients.

share with whomever might find such a protocol useful.

Step 1. Order this gBLOCK synthetic positive control from IDTDNA dot COM

CTACGTGAACCATCACCCTAATCAAGTTTTTTGAGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGATTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGtccatctgaagcgagtcgctTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTACCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCAACTCAATTAGTCAGCAACCAGGTGTGttcggaagatgccagtgtgaCGTTGGCTACCCGTGATATTGCTGAAGAACTTGACGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCACAGCGCATCGCCTTCTATCGCCTTCTTGACGAGaatggacagcgtttgcttaaCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGACTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGACCTGAATGAACTGCAAGACGAGGgccccaaagccctcgtagacCTACAGCGTGAGCTATGAGAAAGCGCCACACTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACAAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGagcatttcacgcgactagccGTTTGTTTGCCGGATCAAGAGCTACCAAATCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATAACAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGtagatctcttcctactagtcCAGCTACCAGACACAGACAAACAGCCCTCGGAGAGACAGAAGCGTGGCCAGCCAGAGCATCATTGCCTACACAATGTCTCTGGGCACCGAGAACAGCGTGGCCTACTCCAACAACTCTATCGCaaaatggcattagcgattgtACATCTGGCTGGGCTTTATCGCCGGACTGATTGCCATCGTGATGATCACAATCATGCTGTGTTGCATGACCAGCTGCTGTAGCTGCCTGAAGGGCTGTTGTAGCTGTGACAGCTGCTGCAAGTTCGACGAGGACGATTacaatcgtacgaaatggaggAGCCTACACCAACAGCTTTACCAGAGGCATGTACTACCCCGACAAGGTGTTCAGATCCAGCGTGCTGCACTATACCCAGGACCTGTTCCTGCCTTTCTTCAgcactctaatgcaaatgcagGATCGCCGACTACAACTACAAGCTGCCCGACGACTTCACAGGCTGTGTGATTGCCTGGAACAGCAACAACCTGGACTCCAAAGTCGACGGCAACTACAATTACCTGTACCGGCTGTTCctgacttctgatcaacctcgCTACATACCTCGCTCTGCTAATCCTGTTACCAGTAGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTAGACTCAAGACGATAGTTACCGGATAAGGCGCtttccgtcccacctggtttaAGATGGCCTACCGGTTCAACGGCATCGGAGTGACACAGAATGTGCTGTACGAGAACCAGAAGCTGATCGCCAACCAGTTCAACAACGCCATCGGCAAGATCCAGGACAGCCTGA Step 1. This is a picture of the gblock standard and the locations of the primers used in the PCR.

Plasmid DNA copy number quantification. protocols, reagents, and a thread of new results. this is a map of the plasmid we sequenced from 2020 batches of mRNA vaccine.

DNA methylation in NORMAL colon can identify who does and does not have colon cancer. its not perfect, but its pretty good. the loci that are differentially methylated between people with and without cancer are the same loci that change dramatically over time as people age. Cancer patients have normal colons that aged too quickly. the developmental biologist will know why this is super interesting. HOX genes have the methylation age clock screwed up.

Friends, put this on blast worldwide.

I will check for DNA integration. Send me de-identified samples and I will PCR test them and sequence any positives.

You and your institution can remain anonymous and avoid the drama. promise.

@DrJBhattacharya @FLSurgeonGen @TheChiefNerd @drdrew

https://x.com/TheChiefNerd/status/1752861883864908221?s=20

one useful type of sample would be archived tumor samples from cancers developing six months or more after exposure.

An open appeal to health care workers around the world. The Pfizer vaccine is contaminated with plasmid DNA. I want to test tissue and blood samples from people recently vaccinated and see if there is any evidence of this DNA integrating into host genomic DNA. PM me if you would like to help. Thanks. -Dr B. but I think that today because of what people are worried about if I checked 20 or 30 cases of myocarditis, and they were all negative it would put some concerns to rest. And that would be a good thing. Alternatively, if I find it in the myocardium, or the pericardium we have an evidence of an easy fix to a serious problem

@Kevin_McKernan @Kevin_McKernan

Pfizer vaccine contains pieces of the DNA plasmid that was used for the in-vitro transcription reaction to make the mRNA. I checked two lots by PCR and sequenced by Oxford Nanopore and verified the findings of @Kevin_McKernan. This does not mean there is a problem, however it… https://t.co/FtEdh40ZK1twitter.com/i/web/status/1…
@JohnGal52793009 @Kevin_McKernan but i looked for human dna just for fun

Raindrops on roses and whiskers on kittens. Bright copper kettles and warm woolen mittens.
Brown paper packages tied up with string. These are a few of my favorite things. Cream colored ponies and crisp apple strudel. Doorbells and sleigh bells and schnitzel with noodles.
Wild geese that fly with the moon on their wings. These are a few of my favorite things.

A (hopefully-useful) thread about PCR testing for SARS-CoV-2.
1. Positive signals have a range of positivity to them. from 1 molecule detected to a billion molecules detected. 2. when someone is first infected, their signal is very low. either zero, or 1 to 10 molecules detected. it goes up over 7 days and then goes back down.

We don't read and write poetry because it's cute. We read and write poetry because we are members of the human race. And the human race is filled with passion. And medicine, law, business, engineering, these are noble pursuits and necessary to sustain life. But poetry, beauty, romance, love, these are what we stay alive for.

the process of science is messy. until recently it has been hidden from public view, with only iron-clad proven ideas making it out for public consumption. now, with biorxiv, and twitter, and a damn pandemic freaking everyone out, the public is being exposed to the process and its a bit surprising to people how science is set up to have wrong ideas articulated and then put in a crucible of scientific process to see what remains. this is normal, its just not been in public until now.

Omicrons (B.1.1.529) most recent common ancestor is AV.1. Relative to this MRCA, Omicron has 25 nonsynonymous and 1 synonymous mutation in Spike, and 13 nonsynonymous and 6 synonymous mutations in the entire rest of the genome. This is very strange & I don't understand. I have re-done the alignments and counting up of mutations, and it's even weirder than i first said.

So here is what happened.
Harvest bat viruses from Yunnan. Transport to Wuhan. Clone SPIKE genes into pseudoviruses. Forward mutagenesis of spike binding to human ace2. Select winner. Insert FCS. Put in bat sars-related backbone. Lab worker gets infected. World catches fire.

https://twitter.com/TheSeeker268/status/1439943910390845448

Eco health and NIAID totally enabled and knew about this dumbassery. Not a sinister plan by China to take over the world with bat viruses. USA is proudly leading the way in stupid. @R_H_Ebright @angie_rasmussen @PeterDaszak did a single virologist ever watch Jurassic Park? WTF?