Papa to 9 microbes, mackerel snapper, farmer, master of sucking. Opinions my own but should be yours. Actually a nice guy.
Oct 13, 2020 • 12 tweets • 6 min read
Wellll... A few weeks back I started working on a tutorial for our lab's Code Club on how to make shitty graphs. It was too dispiriting and I balked. A twitter workshop with figures and code:
Please stop using the open (and closed) reference clustering methods based on USEARCH/VSEARCH. Instead, use de novo clustering algorithms to form OTUs 1/
Open/closed reference methods were developed because people have the type of crappy data that you get when reads don't fully overlap. High error rates increase the number of uniques making downstream processing more difficult 2/ blog.mothur.org/2014/09/11/Why…