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Rado Danev Profile picture
Rado Danev
@RadoDanev
Hi
Jul 25, 2018 • 5 tweets • 3 min read
1.62 Å from the first post-installation test dataset of our Krios @UTokyo_News_en. Thanks to the superpowers of #Relion3, @SjorsScheres @biochem_fan. Also thanks to a great apoferritin prep by Yanagisawa-san from @mkikkawa lab. Dataset details:
- Falcon 3EC, subframe alignment
- x155k, NanoProbe
- Spot 6
- Pixel size 0.52 A
- Exposure rate 0.74 e/pix/s
- Total dose 50 e/A2
- Fractions (frames) 59
- Exp. time 18.21 s
- Defocus range 0.3 - 1.2 um
- Micrographs 1,647 total, 1,297 used
- 190k picked picls