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Nasser Rusan 🔬 ناصر الروسان Profile picture
Nasser Rusan 🔬 ناصر الروسان
@RusanLab
Senior Investigator. Cell and Developmental Biologist Scoping Out 🔬 Mitosis & Centrosomes in Drosophila 🐝(close enough).
Dec 17, 2019 • 7 tweets • 4 min read
A solubility screen to identify centrosome protein complexes

Our collaboration with @GregoryCRogers1 is online @JCellBiol and I wanted to highlight one aspect that is somewhat buried, but took my lab nearly 2 years to complete

the pExpress-Dual screen

rupress.org/jcb/article/21… Frustrated by years of failed centrosome protein purifications for biochemical assays and crystallography, we realized that - proteins need their partners to be HAPPY - . So Sarah Speed, a postbac, designed a screen to isolation soluble centrosome protein complexes Image