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Rahul Samant Profile picture
Rahul Samant
@SamantLab
Group Leader @BabrahamInst. He/him. All things #ubiquitin #proteostasis #proteomics #ageing #senescence. Spend too much on food & wine. Opinions mine, all mine!
Sep 27, 2021 • 23 tweets • 9 min read
As promised, a 🧵 to convince you to consider SP4 for your next #proteomics sample prep!
TL;DR: Cell lysate -> pure peptides ready for LC-MS with 20-30 min handling time w/o any filters (FASP, iST, S-Trap) or specialist beads (SP3). 1/n
biorxiv.org/content/10.110… @biorxivpreprint Image Starting off a lab (babraham.ac.uk/our-research/s…) where proteomics was going to be a major approach, our first recruit—postdoc @HarveyEJohnston—set off to craft our work-horse sample prep work-flow.
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