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Silas Daniel Kieser Profile picture
Silas Daniel Kieser
@SilasKieser
Husband, Father & Embedded bioinformatician PhD candidate
Sep 18, 2020 • 9 tweets • 4 min read
A colleague asked me for some advice about 16S rDNA sequencing... So here is what I've learned during my PhD even when I worked more with Metagenomics than with amplicon sequencing. Constructive additions are welcome. Traditionally, 16S amplicon sequences were clustered at 97% to create operational taxonomic units (OTUs). A unit that corresponds more or less to species, so we thought. academic.oup.com/bioinformatics…