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Terkild Brink Buus Profile picture
Terkild Brink Buus
@TerkildB
Assistant Professor intrigued by skin immunology and single-cell analyses at @skinUCPH.
Jun 19, 2020 • 9 tweets • 7 min read
Running (EC)CITE-seq? #scRNAseq

Can I use FACS staining protocol? Should #CITEseq Abs be used at saturating conc? Can I reduce staining volume? Does cell# at staining matter? How can I minimize Background?

Tweetorial of biorxiv.org/content/10.110…
@SBKoralov @skinUCPH @NYGCtech When setting up #ECCITEseq we encountered many questions that we could not find answers to. Figuring we were not alone, we tested several conditions (tissue/conc./volume/cell #) and compiled the results into a manuscript that we hope will assist others!

biorxiv.org/content/10.110…