Excited to show our latest attempt at a clinically meaningful clinical cancer genome with rapid turn around (24 hours!) times using Oxford Nanopore sequencing technology for patients who need a rapid, whole genome assessment of their tumour and germline, offering them (1/n): 👉  Full germline report
👉Unbiased mutational signatures (for therapy) – e.g. HRD
👉Germline variants and PGX (e.g. DYPD)
👉Genomewide SV profiling at the DNA level (e.g. fusions)
👉Reporting of TMB & MSI status for IO
👉Methylation classification if applicable

So, as promised, here are the observations of @beggs_lab at @unibirmingham about the new LSK114 kit on the R10.4.1 "Marathon" flow cells - we're happy enough that we have put 4 samples on this afternoon on our @nanopore P24 - ready for London Calling 22 - wet lab by @JoStockton1 Firstly - output - this is just a representative example but on our tumour samples we are seeing between 80-120gbases of data generated per sample. This is with LSK114 prep, Covaris sheared to ~10kb. Run for 72hrs. Note the data output - much higher thanks to Marathon flow cells

We’re currently in the midst of setting up a Coronavirus testing lab at @beggs_lab @unibirmingham. For those of you who wonder why things are so slow to test in the UK here are some answers:
1) Primer supply is slow: all suppliers are inundated at the moment. 2) Reagents are slow: RTQPCR master mix is difficult to get hold of and when it does come, ship dates aren’t guaranteed
3) Going from dry swab to extracted nucleic acids is hard - doing it safely at BSL3 is a particular challenge at moment