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Andrew Beggs Profile picture
Andrew Beggs
@adbeggs
Prof Cancer Genetics & Surgery @unibirmingham, Colorectal Surgeon @uhbtrust, “General” scientist as well as general surgeon. Cancer/Surgery/Genomics.
May 18, 2022 • 13 tweets • 6 min read
So, as promised, here are the observations of @beggs_lab at @unibirmingham about the new LSK114 kit on the R10.4.1 "Marathon" flow cells - we're happy enough that we have put 4 samples on this afternoon on our @nanopore P24 - ready for London Calling 22 - wet lab by @JoStockton1 Image Firstly - output - this is just a representative example but on our tumour samples we are seeing between 80-120gbases of data generated per sample. This is with LSK114 prep, Covaris sheared to ~10kb. Run for 72hrs. Note the data output - much higher thanks to Marathon flow cells Image
Mar 30, 2020 • 7 tweets • 3 min read
We’re currently in the midst of setting up a Coronavirus testing lab at @beggs_lab @unibirmingham. For those of you who wonder why things are so slow to test in the UK here are some answers:
1) Primer supply is slow: all suppliers are inundated at the moment. 2) Reagents are slow: RTQPCR master mix is difficult to get hold of and when it does come, ship dates aren’t guaranteed
3) Going from dry swab to extracted nucleic acids is hard - doing it safely at BSL3 is a particular challenge at moment