Allulose is perhaps the most longevity-friendly sweetener. 🧵

Here's why:
+ it's safe
+ multiple health benefits
+ reduced blood glucose
+ metabolic benefits including fat loss, increased GLP1 secretion, liver health, teeth protection. 0/ What is Allulose?
Allulose is a rare sugar found naturally in fruits like figs and raisins. It is nearly calorie-free (0.2-0.4 kcal/g) because it is absorbed and excreted through urine without being metabolized by the human body. The minimal calorie contribution is attributed to limited metabolism by gut bacteria.

First, I shocked my Johnson and performance improved.
Then I shocked my sprained ankle and recovered faster.
Now, I’m shocking my entire body…
🧵 0/ This technology (@SoftwaveTRT) delivers unfocused electro-hydraulic shock waves dispersed over a broad tissue volume. The acoustic pulse passes non-invasively through skin, generating shear forces and micro-cavitation that set off mechanotransduction. Cells release ATP, open stretch-activated ion channels and trigger ERK/Akt signaling.

Within 30 minutes to 24 hours, endothelial-NO synthase and VEGF rise, micro-perfusion improves, and mesenchymal stem cells migrate to the site over subsequent days. End result: increased blood flow, pro-healing growth-factor signaling and activated resident stem-cell repair machinery.

Clinical trials show strong pain relief and faster healing for plantar-fasciitis heels, calcified shoulder tendons and improved blood flow for erectile dysfunction; decent evidence for tennis elbow,Achilles issues and chronic wounds; early data for nerve pain and chronic wounds.

Is sauna worth the hype?

There's evidence that sauna is a potential detox and longevity intervention with benefits to heart, metabolism, brain health, and skin. We’re going to put it to the test.

Here’s what you need to know…🧵 0/ How it works…

Heat exposure in a sauna puts your body under mild stress, triggering natural repair processes that improve overall health. Finnish dry saunas specifically use controlled heat to stimulate these beneficial responses, including:

+ Repairing and maintaining proteins (heat shock proteins)
+ Improving blood flow and relaxing blood vessels
+ Boosting antioxidant activity
+ Optimizing metabolism
+ Enhancing fitness
+ Reducing inflammation
+ Strengthening immunity
+ Recycling and repairing cells (autophagy)

All these processes combine to give whole-body health benefits.

This marker reveals how fast you’re aging.

+ New science just emerged to support it
+ It’s based on DNA methylation
+ It allows me to know what is and isn’t working

Here’s an overview of how:
+ it works
+ you can find out your result
+ you can compare your results to others 0/ Speed of aging (PACE) is one of the most clinically validated aging markers.

My team and I use it extensively to measure the efficacy of health protocols.

This thread explains how biological aging clocks gained a bad reputation and how two new pre-prints take a new approach to restore confidence to the field.

My Vit D levels spiked 235% post HBOT.

My Vit D has been stable for years ~40 ng/mL and then spiked to 134 ng/mL after 60 sessions of HBOT.

For context: I’ve been taking ~5000 IU/day of Vit D for the past few years. My serum levels have consistently hovered around ~40 ng/mL.

Sep 2024 (pre-HBOT): 40 ng/mL
Jan 15, 2025: 67.8 ng/mL
Mar 26, 2025: 110 ng/mL
Apr 1, 2025: 134 ng/mL

Levels approaching 100 ng/mL can carry potential benefits. Beyond that is not ideal.

The threshold of toxicity sits around 150 ng/mL, and while I’m still comfortably below it, I’ve chosen to pause my 5000 IU/day dose to try and normalize levels.

I’m not concerned. Bone health is excellent (top 99.8 percentile via DEXA) and my serum calcium (9.4 mg/dL) is within the upper normal range.

So why did Vit D levels spike? We have a hypothesis.
🧵 1/ Improved intestinal bile acids

Through reversal of dysbiosis and increased growth of healthy gut bacteria.

How does it affect vitamin D?

Bile acid metabolites (especially LCA) promoted by a healthy microbiome promote vitamin D absorption into circulation.

I take 2.5 mg of Cialis daily.
Not for erections, but for longevity.

Here’s what the science says about Cialis and lifespan extension…

And why it’s not the reason behind my 3 hour long titanic nighttime erections.
🧵 0/ It sounds wild, but low-dose Cialis (tadalafil) and Viagra (sildenafil) are both drawing attention for longevity benefits.

Recent clinical evidence indicates the use of either correlates to longer survival in men, in addition to their potential for heart health, metabolism, and even brain function.

A new retrospective study evaluated the longevity potential of 407 prescription drugs in over 200,000 participants, with both Cialis and Viagra being among the surprising promising hits in men (yet to be evaluated in women, since the retrospective studies only cover existing drug use for established indications).

Sildenafil will soon be tested in the NIA’s ITP, the most credible pre-clinical longevity drug testing program in mice.

Study uses MRI ad machine learning to estimate brain age, and identifies 64 “druggable” genes that drive brain aging 0/ Published last week in @ScienceAdvances a study reported the use of brain scans with multiple deep learning models combined with gene expression analysis in blood samples and brain tissues from to identify genes that drive brain aging.

My sperm quality equal to a healthy 20-year-old.

+ sperm 330 million
+ motility 53%
+ motile sperm 175 million
+ morphology 10% normal

Sperm health predicts testosterone, metabolic health, disease, addiction, and life expectancy. 

Here’s what you need to know.🧵 0/ I have more motile sperm than the average healthy 22-29 year olds and much higher than the average fertile US man (104 million motile sperm count for the US father, age 32∓6 years).

Image above of my sperm is from a 2023 test.

I am looking into exosomes from human trophoblast stem cells (hTSC) given their regenerative potential such as anti-inflammatory effects, metabolic optimization, telomere lengthening, and cellular senescence reversal.🧵 0/ Exosomes are nano-sized extracellular vesicles released by almost all cell types. They carry bioactive cargo (RNAs, proteins, lipids) and mediate intercellular communication, influencing many cellular processes like tissue repair, inflammation, and aging.

HBOT ranks as one of the highest value health therapies I’ve done. Results from 60 sessions:

+ wiped out all systematic inflammation in my body. Below detectable levels. This is wild.
+ 300% increase in VEGF (formation of new blood vessels)
+ telomerase activity of a 12 year old, associated with biological age
+ 250% and 290% increase in Short-Chain Fatty Acids and n-Butyrate, respectively, important microbiome markers
+ complete elimination of metabolic imbalance in my gut
+ 28.6% reduction in a dementia marker
+ improved muscle oxygenation
+ dramatic improvements to whole body skin health

The outcomes match what we observed in the scientific literature and what we predicted in deciding to do this therapy.

What's notable is that after achieving elite level biomarkers over the past four years, my team and I have struggled to find new therapies that meaningfully improve my biomarkers. HBOT achieved that.
🧵 0/ Inflammation

Post HBOT, there’s no detectable systemic inflammation in my body (hsCRP). Inflammation is the foundation of disease and all things bad health.  Before HBOT, my inflammation markers were in the top 5% of 18-29 year olds (you get more inflamed as you age).

HBOT eliminated systematic inflammation in my body to a point it was below levels of detection. This is wild.

A second test to confirm the inflammation measurement, my CRPm, an epigenetic biomarker proxy for inflammation, is in the lowest 1%.

Dear humanity,

I am building a religion.

Wait a second, I know what you’re going to say. Hold that knee-jerk reaction and let me explain.

First, here’s what’s going to happen:
+ Don’t Die becomes history's fastest-growing ideology.
+ It saves the human race.
+ And ushers in an existence more spectacular than we can imagine.

It is inevitable. The only question is: will you be an early or late adopter? Why is it inevitable?

Four fundamental shifts are happening right now:
1) We are giving birth to superintelligence (AI).
2) No existing ideology solves AI alignment.
3) With AI, our species’ survival is not guaranteed.
4) With AI, individual biological death is no longer inevitable.

We are at risk of extinction without a unifying framework to solve human alignment and AI alignment.

I am aging slower than anyone in the world.
#1 in a fierce competition among 5,677.
My score is off the charts.
And I'm genetically disadvantaged.

What does this even mean? 🧵 0/ Biological Aging is mostly driven by the environment, habits and lifestyle choices and is malleable to interventions.

Unlike chronological aging, biological aging is malleable and is a direct outcome of your behaviour and lifestyle choices. A seminal study in over 2000 twins (gold standard for studying genetic effects) found that longevity was only 25% hereditary.

In other words, your genes determine only 25% of your biological aging speed and patterns, while the rest (75%) is up to you to decide and influence.

Speed of aging is the most dynamically responsive as it relies on estimating the speed of aging from a snapshot in time, hence allowing a quick intervention-measurement loop, allowing for the fast evaluation of therapies and protocol for their effectiveness as rejuvenation interventions.

Biological Age will normally follow the speed of aging, with some considerable delay for trends to appear.

Imagine a car changing its speed, you will be able to see the change directly at the speedometer (speed of aging) much faster than seeing when you reach your destination based on the new speed.

People living in hotter climates age faster

Published this week a study used three distinct epigenetic aging clocks to show that more hotter days on average (caution-level and extreme level heat) make you age faster. 🧵 Background

0/ The study tracked biological age in 3686 adults over 56 years across the US in relation to outdoor ambient heat exposure.

The LA fires filled my body with harmful toxins.

One toxic chemical, NAPR, an organic solvent used in metal cleaning, foam gluing, and dry cleaning, increased by 728%.

If you were exposed too, here is what you can do. 🧵 0/ I suppose if there was one person in LA who was routinely measuring their toxin levels when the fires broke out, it would be me.

My urinary toxins panel taken at the height of the LA wildfires on January 28th, 2025 showed 9 acutely increased metabolite toxins (4 of which above the 95th percentile and the other 5 between the 75th and 95th percentile).

A similar previous panel taken in October 2024 had all these toxin metabolites within normal or undetectable range.

Note, in-between the baseline test of Oct 2024 and the most recent test of Jan 28th, I spent a week in India which may have also contributed to an increased toxin load. More on that below.

Let us take a closer look at these metabolites, what toxic exposures and health hazards they indicate, and how these tie to wildfire and burning homes, as well as air pollution in general.

Let us also examine efficient approaches to the detoxification of wildfire fumes inhalation.

The New York Times is preparing to publish a hit piece on me.

I found out this story is running because a reporter from NYT emailed me and my colleagues her “fact-checking” questions. This is standard practice before publishing a story.

“I am working on a story about Bryan Johnson and his weaponization of non-disclosure agreements over the years to cover a range of bad behavior…”

This journalist is trying to construct a narrative about me.

Let’s take a look at what she’s trying to do.🧵 Step 0: Motive.

Why is The New York Times coming after me? It’s a clash of self-serving narratives:

1. The New York Times wants a sensational story. Clicks = money.
2. The journalist wants to “take down” another target.
3. My ex is chasing clout by recycling allegations that have already been rejected in two legal forums.
4. Don’t Die is growing fast, threatening status-quo power. This makes me a target.

I’m fortunate that I have social reach. But what’s really unfair is when this happens to people who have limited recourse.

This isn’t just about me, this is about how the media manufactures reality. If a journalist is going to try and construct a narrative about any one of us, we should evaluate the foundation it’s built on.

Last week, @chamath and I had dinner. He had this to say:

"Bryan's skin is incredible. His skin is like a porcelain dolls. Both my wife and I were like, we've never seen skin like this. It's incredibly soft. It's the softest skin I've ever touched in my life."

Supple skin is proof of young biological age, and a power-law marker of systemic health and efficient rejuvenation.

Let's look at the science 🧵 0/ Supple skin is proof of young biological age, and a power-law marker of systemic health and efficient rejuvenation

Achieving supple skin at 47 is far more than a cosmetic milestone—it's a potent, integrative indicator of overall health. In many biological systems, vital functions follow a power-law distribution, where small improvements in underlying systems can result in disproportionately large benefits in overall function.

I injected the horse tranquilizer Ketamine and tracked my brain data for 15 days. It completely scrambled my brain.

In a world-first we answered the question ‘what happens to the brain before, during, and after ketamine treatment?’ We also discovered how long it took for my brain to return to ‘normal’.

The results surprised me. 🧵 0/ Before taking ketamine, my brain activity followed fixed, predictable patterns, as observed over 10 days of measurement. Imagine the brain as a global air traffic network, where each airport (or brain region) has consistent flight routes and traffic volumes—like the steady flow of planes between New York and London.

After ketamine, my brain’s activity patterns were completely scrambled. Instead of predictable routes between major hubs, traffic was rerouted to smaller, less-used airports across the U.S., Europe, and Asia.

This means brain activity that was once rigidly structured became more flexible and varied, potentially unlocking new connections and ways of thinking.

Top images: My absolute functional connectivity (brain traffic patterns) for minutes 5-15 after I received 57.75mg intramuscular injection of ketamine.

Bottom images: My relative functional connectivity (brain traffic patterns) changes after the ketamine took effect, starting at minutes 15-25.

Love and deep social bonds can reduce mortality risk by up to 91%.

A landmark meta-analysis with 309k participants found meaningful and complex social relationships reduced mortality risk significantly.

Happy Valentine's Day. 🧵 0/ Close relationships may predict longevity better than wealth, status, IQ, or even genetics.

+ An 80-year Harvard study found that deep social bonds are the strongest determinant of long, happy lives. The effect transcends culture.

+ A study of 97,062 Japanese participants found that never-married men had a 91% higher risk of death; for women, 46%.

+ In Europe, a study of nearly 1 million people found marriage consistently associated with lower mortality across six countries.

+ A meta-analysis of 7.8 million participants across 22 studies showed a 22% and 33% higher mortality risk for unmarried women and men, respectively.

I don't want to do this, but you need to hear this.

Sleep deprivation lowers IQ, increases toxic beta-amyloid buildup, and raises brain damage markers linked to stroke.

Learn how high quality sleep boosts the brain.
🧵 0/ Sleep deprivation is brain damage

Shorter sleep correlated with lower IQ-test performance in school children.

Who’s the best mind in tokenomics?

We’re designing rewards for the Don’t Die ecosystem—earn for hitting health markers, buying DD-approved products, dining at DD-certified restaurants, and more. omg within 5 minutes of this post I received 50+ DMs

I am no longer injecting my son's blood.

I've upgraded to something else: total plasma exchange.

Steps:
1. Take out all blood from body
2. Separate plasma from blood
3. Replace plasma with 5% albumin & IVIG

Here's my bag of plasma. Who wants it?
🧵 0/ Total Plasma Exchange TPE Therapy is an advanced longevity therapy with great effects in removing toxins, waste products, old misfolded and glycated proteins and immune complexes. It has shown measurable effects in delaying and reversing age-related disease including dementia, as well as the capacity to reduce biological age based on various parameters, including blood proteomics and biological aging clocks.