I sometimes see #SingleCell papers where the authors treat some dimensionality reduced plot as ground truth. Here's a quick, simple example showing why that can be problematic. 1/n Here are three random distributions plotted at the vertices of an equilateral triangle. As you can see, the mean distance from any two clusters is equal. 2/n

Our single cell atlas of human B cells is out in @ImmunityCP! We screened the B cell surface proteome and then followed up with functional analyses. We identify twelve populations across four lymphoid tissues. cell.com/immunity/fullt… 1/16 Using a multiplexed mass cytometry approach, we screened the expression of 351 surface molecules on human B cells, mostly looking at CD markers and other proteins associated with immunological function or signaling. 2/16