Why is there so much disorder in eukaryotic proteins? What is its relationship with condensates? Why can some proteins be saved but others get degraded? After years in the making, we think we have answers and they're all connected. Excited to share a 🧵: biorxiv.org/content/10.110…
First some background. 2 y ago we developed a way to measure which proteins can put themselves together after being unfolded with denaturant using LiP-MS. tl;dr some can, some can’t (b/c they need chaperones or prefer to fold on the ribosome). 2/10. pubs.acs.org/doi/10.1021/ja…
Jul 26, 2021 • 7 tweets • 4 min read
Very excited to share that our work on Protein Nonrefoldability is out today in @J_A_C_S! #AlphaFold might be great at finding proteins' native structures... but turns out many proteins themselves are not! Short 🧵1/7 pubs.acs.org/doi/full/10.10…
We developed a mass spec approach to probe the refoldability of the proteome. First we unfold & refold E. coli extracts, then use a protease to interrogate the structures of 'refolded' proteins. The resulting peptide fragments are sequenced by LC-MS & compared to native. 2/7
