Jay Hinton Profile picture
Salmonella admirer - focusing on the African iNTS epidemic, using functional genomics.
26 Aug 20
1/5
African S. Typhimurium ST313 has 23 Lineage 2-specific pseudogenes, discovered by @DrRobKingsley in 2009, inc ybjZ.

But what is function?

In collab with Jared Honeycutt @monack_lab, we've discovered that MacB (YbjZ) influences pathogenesis of ST313

journals.plos.org/plospathogens/…
2/5
Genomes of 285 S. Typhimurium isolates revealed lineage-specific SNPs = macA & macB are pseudogenes

Suggesting that MacAB-TolC efflux pump is functional in S. Typhimurium ST19, but defective in ST313 Lineages 1 & 2
3/5
Beautiful expts designed by Jared Honeycutt & @monack_lab show MacA variant & truncated MacB protein made ST19 sensitive to C18G antimicrobial peptide

& the ST313 macAB SNPs reduced intra-macrophage replication & fitness in murine GI tract

See @monack_lab tweet for details
Read 6 tweets