We've put out a short preprint today showing some updates in Readfish for @nanopore adaptive sampling. biorxiv.org/content/10.110…@alexomics is giving a talk shortly at the #nanoporeconf about this - but a short thread here.
Readfish now exploits the barcoding features ready baked in to @nanopore guppy to allow a few new features. You can switch off barcodes as you wish but more interestingly, you can assign different targets to different barcoded samples.
Sep 17, 2021 • 15 tweets • 9 min read
Earlier this week we put out a couple of preprints (doi.org/10.1101/2021.0…doi.org/10.1101/2021.0…) describing minoTour github.com/LooseLab/minot…, an @nanopore tool we have been developing for several years. A bit of a 🧵
The minoTour design philosophy is all around real time - it's about working out what is happening at that moment - with the exception of ARTIC (see below) we anticipate users running further analysis after minoTour!
Sep 8, 2021 • 12 tweets • 11 min read
An ultra-long read update. Since @circulomics is "no longer available" for @nanopore people might be interested in these protocols from @longreadclub@NininUon and @DrT1973 - a toolkit of options here dx.doi.org/10.17504/proto… including monarch from @NEBiolabs - 🧵below with more.
Ultra-long sequencing (N50s >100kb) jumped in performance in 2020 with new kits from @nanopore and @circulomics giving yields in excess of 20 Gb. In particular massively improved occupancy seemed to be a major benefit.
