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Michael Cianfrocco Profile picture
Michael Cianfrocco
@mcianfro
@UMich Assistant Professor @UMLifeSciences & Dept. of Biological Chemistry / @Cianfrocco_Lab / #cryoem / #biophysics / #motorproteins. he/him/his
Jan 22, 2020 • 8 tweets • 5 min read
New preprint from the lab: "High-resolution cryo-EM using beam-image shift at 200 keV." Take home message: even with a pretty poorly aligned #cryoEM instrument, you can overcome these aberrations computationally with #RELION (1/n) biorxiv.org/content/10.110… This came from me asking many people: what is the limit for using beam-image shift to collect data at 200 keV? Everyone said it should be worse at 200 keV than 300 keV but we were surprised no one had benchmarked this yet