Delighted to share new work from our lab: CRISPR fusion proteins to boost PRECISE genome editing (TruEditors).

We wondered what if we could “turn on” and test every gene in the human genome to accelerate protein design? Recently, there have been several breakthroughs to find solutions to the protein folding problem — most notably, AlphaFold. These efforts map protein sequence to structure.

But what if we want to design proteins with specific functions?

A TWEETORIAL on our new CRISPR-sciATAC preprint. Our lab has been working on combining pooled CRISPR screens with single-cell chromatin accessibility for nearly 4 years so very happy to see this work finally out there in the world.
biorxiv.org/content/10.110… The work was led by 2 talented postdocs in our lab (@noaliscovitch and @antonino_nyc) with support from many other folks in our lab and in @NYGCTech lab. Extra shout-out to @noaliscovitch for getting the manuscript over the final hump in what has been a challenging year.

As promised, here’s an overview of our study to find host factors required for SARS-CoV-2 infection in human cells. We use a genome-wide CRISPR screen to identify genes (& key mechanisms) that might be therapeutically targetable to prevent viral infection. Here’s a link to the manuscript (open access):
cell.com/cell/fulltext/…

Happy to share our new preprint characterizing a recent mutation in SARS-CoV-2 Spike protein (D614G) and its potential functional impact on the ability of the virus to transduce human cells.

https://twitter.com/biorxiv_genetic/status/1272783104919457792

First, this work was spearheaded by a talented postdoc in our group #ZharkoDaniloski and is a wonderful example of curiosity-based science.