Postdoc @Stevens1lab | Interested in Alzheimer’s disease, all things single cell, and glia, glia, glia | Dad 👧👶🏻 | #scicomm #rstats | NEU ‘10 & UCI ’16
Jul 23, 2022 • 16 tweets • 4 min read
Ummm No.
This only reinforces the need for accurate science reporting.
Combination hyperbole and inaccurate reporting fuels misinformation.
Some brief thoughts (bear with the slightly rambling nature 😉) 1/n
The fraud/misconduct appears to be centered around AB*56. As quoted in original Science story, many in AD field already had doubts about AB*56.
ALSO AB*56 is not the only oligomer, this paper while influential was not the basis of the past 16 years of research.
2/n
Mar 8, 2022 • 36 tweets • 12 min read
Thrilled to announce my 1st paper from @stevens1lab is now published @NatureNeuro. “Dissection of Artifactual and Confounding Glial Signatures by scRNA-seq of Mouse and Human Brain”. Why is it important? a 🧵👇 #singlecell#microglia#methodsmatter 1/n nature.com/articles/s4159…
While the title frames this work in terms of brain, a KEY takeaway from new data in this version is that this is broadly applicable across basically all scRNA-seq (and RNA-seq) studies (especially in immunology)... 2/n
Aug 3, 2021 • 28 tweets • 7 min read
As always @LGMartelotto asks the best discussion kickoff questions!! 😀 so here’s a thread (I promise it’s worth full read 😉) on my thoughts and some considerations/caveats for use of transcriptional/translational inhibitor cocktail we describe: biorxiv.org/content/10.110… 1/n
Broadly the tl:dr is:
•scRNA-seq w/enzymes > 4°C = Yes
•scRNA-seq mechanical dissociation > 4°C = Yes
•scRNA-seq mechanical dissociation < 4°C = Not necessary but not harmful either*
•snRNA-seq = Not necessary**
2/n
Dec 3, 2020 • 39 tweets • 15 min read
Excited to share first preprint from my postdoc work in Stevens lab: “Single Cell Sequencing Reveals Glial Specific Responses to Tissue Processing & Enzymatic Dissociation in Mice and Humans” a thread below #singlecell#microglia#methodsmatter 1/n
biorxiv.org/content/10.110…
What started as small internal pilot in the lab, turned into full-fledged paper. I’ll detail some of the key findings in thread below. But first want to take a second and thank all of our fantastic colleagues and collaborators without whom this project wouldn’t be possible 2/n
