Great critique by Billy of a preprint purporting to show a "FCS" in a novel coronavirus from Brazil

I would add that

1) no evidence is presented that the cleavage site is cleaved by furin.. 🧵

https://x.com/BillyBostickson/status/1983865782649155881

In addition, cleavage occurs after secretion into the culture medium, which seems inconsistent with furin as responsible for cleavage during infection

Whether it actually undergoes cleavage as part of its cell entry mechanism is unclear

A link between MERS-CoV pathogenicity and epithelial sodium channel (ENaC) was described in a 2016 proposal by Luis Enjuanes, collaborator of Baric h/t @USRightToKnow

Coincidentally, the human α-ENaC furin cleavage site (FCS) is identical to that of SARS2 🧵

https://twitter.com/jhas5/status/1975400184042402120

2/ As far back as 2009 it was known that SARS1 spike and E proteins interacted with human ENaC, modulating its activity leading to fluid buildup in the lungs

Fluid buildup (pulmonary edema) is a key symptom of both SARS1 and SARS2

journals.physiology.org/doi/full/10.11…

The GOF Executive Order includes no clear ban on US-based GOF research, but kicks the can down the road

Weak gruel unfortunately at this stage, vested interests seem to have inserted themselves 🧵
whitehouse.gov/presidential-a… @thackerpd @emilyakopp @HansMahncke @ban_epp_gofroc @lewiskamb 2/ While executive focus on GOF is to be welcomed, unfortunately this Executive Order (EO) does not deliver a ban on US GOF research. While it bans funding of dangerous GOF in countries of concern, why does it not do the same in the US ? Why not a blanket ban ?

We received an (indirect) response from UNC regarding our preprint outlining evidence for a potential lab leak of SARS2 at UNC

UNC asked GISAID to tell us to remove our preprint

We did this, but have now put up an updated version 🧵
zenodo.org/records/151721… 2/ @quay_dr uploaded our original preprint on March 26

We identified 7 'frozen' SARS2 sequences from UNC, that were basal to other sequences generated at the same time. These could represent early SARS2 strains that had escaped from a research facility

https://x.com/stevenemassey/status/1904619032151810403

Steve Quay @quay_dr and myself have published evidence for a potential lab leak of SARS2 at the University of North Carolina (UNC)

7 genome sequences collected by UNC in 2020-21 are basal (ie 'frozen') indicating a potential lab origin 🧵
zenodo.org/records/150833… 2/ The 7 sequences were collected from Jun 2020 to Jan 2021 and submitted by Dr Dirk Dittmer of the Clinical Molecular Microbiology Laboratory, UNC Hospital

Puzzlingly, they show strong similarities to the SARS2 reference sequence Hu-1, sampled in Dec 2019

I've published a paper on the emerging phenomenon of 'frozen' virus genome sequences, and how they can indicate lab leaks

"Frozen' virus genome sequences are generated from recent outbreaks, but show surprising similarity to historic strains 🧵
mdpi.com/2076-2607/12/1… 2/ The classic example is 1977 'Russian' H1N1 Flu, which re-emerged after having gone extinct in the 50s. Genomic sequences were identical to strains from the 50s, indicating it had been stored for > 20 years before release
nature.com/articles/27433…

It has been reported German Intel has assessed that COVID19 originated via a lab leak

Swiss paper NZZ reports that our discovery of a MERS-related infectious clone with a chimeric spike from Wuhan sequencing datasets was considered by German Intel 🧵 2/ The study by @humblesci @Daoyu15 @BiophysicsFL @ydeigin @quay_dr and myself was published last year and demonstrated unreported GOF experimentation on novel MERS-related coronaviruses in Wuhan prior to the pandemic
fortunejournals.com/articles/disco…

Pekar et al have published a paper claiming that 2 SARS2 A/B intermediates from Feb 2020 are derived rather than ancestral

The existence of ancestral intermediates would falsify Pekar et al's earlier claim of 2 independent spillovers of lineages A and B at the Huanan Market 🧵 2/ Hence Pekar et al undergo extraordinary convolutions in order to try and claim the 2 A/B intermediates, identified by Lv et al 2024, are late arrivals rather than the original intermediates that led to the evolution of lineage B from lineage A

academic.oup.com/ve/article/10/…

'Conincidentally' we showed pUC57 was also used to clone a genome fragment from an unpublished pangolin-related SARS2r-CoV from China 🧵

h/t @humblesci @Daoyu15 @ydeigin @quay_dr

mdpi.com/2673-8007/2/4/…

x.com/Engineer2The/s… 2/ This striking conincidence indicates that the novel pangolin-related coronavirus we identified from contaminated datasets (GX_ZX45r-CoV) was likely part of an infectious clone (IC), given that one of the reads was ligated to pUC57

From our paper:

I have published a paper on my analysis of the RaTG13 dataset

RaTG13 is the closest phylogenomic relative of SARS2

Transcriptome and GO enrichment analysis indicates the likely source of the dataset is a bat mating plug, rather than a fecal swab 🧵

mdpi.com/2036-7481/15/3… A mating plug provenance resolves the long standing conundrum over the low amounts of bacteria in the RaTG13 sample, noted by @MonaRahalkar and others, which is inconsistent with a fecal swab as reported by the WIV, who collected the sample

A leak of polio from a research facility is indicated by a poliovirus genome sequence generated from a sample collected in 2014 in China by the Wuhan Institute of Virology

The sequence is 99 % identical to that of a polio strain from 1954 🧵
academic.oup.com/ve/article/10/… 2/ Chesnais et al sequenced 3 poliovirus genomes recovered from 60 year old historic samples generated by Albert Sabin at the Pasteur Institute, Paris

Sabin is the father of the oral polio vaccine, created from attenuated polio strains

Why are virologists freely allowed to anonymize lethal synthetic viruses, but developers are put in jail for writing code that anonymize bitcoin transactions ?

Genetically enhanced infectious clones present a much higher risk than anonymous monetary transactions 🧵 @R_H_Ebright @SenGaryPeters @COVIDSelect @BiosafetyNow @CharlesRixey @HSGAC_GOP @RepBradWenstrup @RepRaulRuizMD Virologists often synthesize infectious clones (ICs), which are used to produce live infectious viruses

To make an IC of a coronavirus, due to its large size constituent fragments need to be synthesized, and then ligated together to form a complete genome

Unravelling the RaTG13 Dataset's Origin

The RaTG13 dataset has overexpressed transcripts which indicate it was mis-sampled from a bat mating plug

This provides a reasonable explanation for anomalies in the dataset, described as a 'fecal swab' 🧵 2/ RaTG13 is (still) phylogenomically the closest coronavirus backbone to SARS2

There has been a longstanding controversy regarding the origin of the RaTG13 dataset

RaTG13 is described as being generated from a Rhinolophus affinis (Intermediate Horseshoe bat) fecal swab

Re-watching this video, it becomes clear why virologists have lobbied so hard to detach the names of novel pathogens / strains from the location where they arise

"the disease is the same name as the lab" 😆

https://twitter.com/stclairashley/status/1803952107902456202

Detaching regional / place names from pathogens is a form of anonymization eg delta, omega etc

Why would virologists want to use names which are arbitrary and non-informative ? geographic information is an important part of epidemiology and helps understand origin and spread

Some further insights into the SARS2 spike sequences found in Pseudomonas aeruginosa datasets, recorded as being sampled in 2019 🧵 2/ Complete SARS2 spike gene sequences were found in contigs generated from Pseudomonas aeruginosa cultures sampled in 2019, by @iximeno

The spike sequence displayed codon optimization and lacked the furin cleavage site

Differential gene expression analysis of the controversial RaTG13 dataset reveals strong similarity to the RaTG15 dataset, also described as generated from a Rhinolophus affinis 'rectal swab' from the Mojiang Mine

This indicates they have a common, undefined source 🧵 2/ The source of the RaTG13 dataset has been a key puzzle of the C19 Origin debate

RaTG13 was sequenced by the Wuhan Institute of Virology prepandemic in 2017/2018 and remains the closest related CoV backbone to SARS2

The Zhang group of Fudan University have identified and validated two A-B intermediate SARS2 genomes from the early pandemic

This provides a key to understanding the origin of COVID19 🧵 2/ In their new paper, the Zhang group sequence 343 new SARS2 genomes from the early pandemic (sampled up to Oct 2020). The genomes were obtained from COVID19 patients in the Shanghai Public Health Center
academic.oup.com/ve/advance-art…

Was Baric aware of the work on the human α-ENaC furin cleavage site (FCS) at the University of North Carolina (UNC) ? 🧵

In a striking coincidence, the human α-ENaC FCS is exactly the same as that of SARS2, as first noted by Anand et al in 2020
elifesciences.org/articles/58603 2/ Furin cleavage of human α-ENaC has been studied by M.Jackson Stutts, who is based at the UNC School of Medicine

Ralph Baric is also based at UNC School of Medicine, and also has an interest in lung disease. Was he aware of this work ?

rupress.org/jgp/article/15…

What was the restriction enzyme BsmBI, itemized in the DEFUSE budget, to be used for? 🧵

3 possibilities:

i) To make an spike ectodomain expression vector
ii) To insert recombinant S genes into VRP vectors
iii) To make infectious clones (ICs) 2/ I will consider each of these possibilities as follows

A potential explanation for the alanine (A684) in the SARS2 FCS ?

Alanine mutational scanning is used to systematically mutate residues in functional sites, to determine if they ablate function, so indicating their importance 🧵

en.wikipedia.org/wiki/Alanine_s… 2/ In the recent FOIA-ed DEFUSE documents released by @emilyakopp and @USRightToKnow there is a section that proposes to 'ablate' 'human-specific cleavage sites' introduced into SARSr-CoV spike proteins

Bombshell !

From an early draft of DEFUSE:

"The BSL-2 nature of work on SARSr-CoVs makes our system highly cost-effective relative to other bat-virus systems"

https://twitter.com/emilyakopp/status/1736848388350459913

Baric was aware of the lax safety standards at the WIV, in the following draft note:

" In china, might be growin these virus under bsl2. US reseachers will likely freak out"

But put his name to the proposal anyway