The peer-reviewed version of expansion in situ genome sequencing is now out in Science!

The news is bittersweet – when we first revealed this last September, I never guessed it would be my final paper in academia, but a lot has changed. A few parting thoughts: This project started as nothing more than a crazy idea: that we could directly sequence genomes inside the nucleus

It would never fly anywhere but academia, where we spent 7 painstaking years making it a reality

Just released @Nature: slide-DNA-seq, our new method for spatial DNA sequencing in tissues 🧬

Excited to add a genetic dimension to the rapidly expanding spatial toolbox with the tech dev superteam of Tongtong Zhao, @JD_Buenrostro & @insitubiology (1/6)

nature.com/articles/s4158… Previously, @SGRodriques, Bob Stickels, @insitubiology, & @macosko invented Slide-seq, which leverages DNA-barcoded beads for high-res spatial transcriptomics

slide-DNA-seq uses the same beads as the basis of a Tn5-based library construction strategy developed by Tongtong (2/6)

Introducing IN SITU GENOME SEQUENCING, a new technology to resolve DNA sequence🧬 and structure🔬

A team effort with @Andrew_C_Payne, @PaulReginato, @eboyden3, @JD_Buenrostro, and Fei Chen

science.sciencemag.org/content/early/…

This is how it works: (1/8) We started with a basic question: how can we sequence DNA while preserving 3D genome structure?

Instead of extracting DNA, we developed a method to construct sequencing libraries within fixed nuclei (2/8)