1. The Yeast is Red and Many are Dead Thread
Sub Yeast Thread 1
2. via @ydeigin and @franciscodeasis
it’s a very disturbing paper if its findings are verified: not only SARS2 and RaTG13 have signs of genetic manipulation in yeast but SARS1 too.
WIV is known to have eyed yeast reverse genetic systems with interest (see attached WIV thesis
3. The Author has published quite a few interesting papers with 1870 citations.
scholar.google.com/citations?user…
One on the Association between influenza vaccination rates and SARS-CoV-2 outbreak infection rates in OECD countries
papers.ssrn.com/sol3/papers.cf…
4. Random Yeast Papers - WIV
Construction and Rescue of a Functional Synthetic Baculovirus
pubs.acs.org/doi/10.1021/ac…
5. Honours in Yeast (08/2020) - Yeast Virus Cookbook
Every Tweet is one Tab Less ;)
In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline
honours.eu/publications/i…
and pdf:
ncbi.nlm.nih.gov/pmc/articles/P…
6. Yeast containing SARS-COV- 2
“The exciting thing about the yeast is that . . . it’s fast,” says expert Susan Weiss
Scientists Clone SARS-CoV-2 Genome with Quick Yeast-Based Method (May 2020)
the-scientist.com/news-opinion/s…
7. Dr. Weiss's Magic Yeast
What this means is that instead of having to reconstruct the viral DNA first before introducing it into cells, you instead “put all these fragments in the yeast and it magically puts them together,” says Weiss.
8. TAR, YAC & DNA
transformation associated recombination (TAR)
yeast artificial chromosome (YAC)
Data Not Available (DNA) Don't believe me? Ask WIV!
9. Amazing Recombination Capacity
“The capacity to recombine things to a YAC by themselves is just amazing,” says Jores.
10. In Vitro Ligation
Refers to this UTMB paper:
An Infectious cDNA Clone of SARS-CoV-2
cell.com/cell-host-micr…
Note: Pei Yong Shi and James Le Duc
11. Method from above UTMB paper
An Infectious cDNA Clone of SARS-CoV-2
cell.com/cell-host-micr…
12. One Week Is All It Takes
Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform
nature.com/articles/s4158…
13. Nice Clear Layman's Explanation (Thank God)
Researchers develop synthetic novel coronavirus clones
phys.org/news/2020-05-s…
14. Well illustrated background reading
Billy Recommends: More Yeast with your Whining!
Synthetic genome engineering forging new frontiers for wine yeast
tandfonline.com/doi/full/10.10…
15. Virus Like Particle Vaccine Manufacture from Yeast
(Illustrated Master's Thesis) 2020
digitalcommons.mtu.edu/cgi/viewconten…
16. Synthetic viruses—Anything new?
asks Volker Thiel in 2018
The generation of synthetic poxviruses as described by Evans and colleagues and cloning of synthetic DNA using TAR in yeast are illustrated
journals.plos.org/plospathogens/…
17. Yeast-based synthetic genomics platform to genetically reconstruct RNA viruses exist.
How much were they used by Chinese Researchers in the years leading up to the pandemic is an interesting research project.
18. WIV Research (2018) - Yeast
Advances and challenges in enveloped virus-like particle (VLP)-based vaccines
Shiyu Dai, Hualin Wang, Fei Deng
State Key laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan
immunologyresearchjournal.com/public/article…
20. Revenge of Yeast
a WIV Thesis that @TheSeeker268 found recently is by Lei Ping Zeng (2017) & it mentions WIV's interest in yeast recombination methods
Reverse Genetic System of Bat SARS-like Coronaviruses & Function of ORFX
drive.google.com/drive/folders/…
21. Zeng Translated
(by @TheSeeker268 & @franciscodeasis )
docs.google.com/document/d/1uZ…
Only 2 references to yeast recombination but obviously on their radar back in 2017.
22. Across ocean & back in time to Wizard of UNC
Thanks Jim Haslam @jhas5
Yeast Based Small Molecule Screen for Inhibitors of SARS-CoV
journals.plos.org/plosone/articl…
23. I am getting so sick of Yeast!
I will just dump yeast infected papers here for others to vomit over
Yeast for virus research (2017) Richard Yuqi Zhao
ncbi.nlm.nih.gov/pmc/articles/P…
24. Enough Yeast!
2007: Saccharomyces cerevisiae: a versatile eukaryotic system in virology
…robialcellfactories.biomedcentral.com/articles/10.11…
2010: A High-Throughput Approach for Studying Virus Replication in Yeast
…rentprotocols.onlinelibrary.wiley.com/doi/10.1002/97…
25. I will finish with yeast until i get more yeastinspiration
Share this Scrolly Tale with your friends.
A Scrolly Tale is a new way to read Twitter threads with a more visually immersive experience.
Discover more beautiful Scrolly Tales like this.