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Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Jun 10, 2021 • 25 tweets • 18 min read • Read on X
Scrolly
1. The Yeast is Red and Many are Dead Thread
Sub Yeast Thread 1
2. via @ydeigin and @franciscodeasis
it’s a very disturbing paper if its findings are verified: not only SARS2 and RaTG13 have signs of genetic manipulation in yeast but SARS1 too.
WIV is known to have eyed yeast reverse genetic systems with interest (see attached WIV thesis
3. The Author has published quite a few interesting papers with 1870 citations.
scholar.google.com/citations?user…
One on the Association between influenza vaccination rates and SARS-CoV-2 outbreak infection rates in OECD countries
papers.ssrn.com/sol3/papers.cf…
4. Random Yeast Papers - WIV
Construction and Rescue of a Functional Synthetic Baculovirus
pubs.acs.org/doi/10.1021/ac…
5. Honours in Yeast (08/2020) - Yeast Virus Cookbook
Every Tweet is one Tab Less ;)
In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline
honours.eu/publications/i…
and pdf:
ncbi.nlm.nih.gov/pmc/articles/P…
6. Yeast containing SARS-COV- 2
“The exciting thing about the yeast is that . . . it’s fast,” says expert Susan Weiss
Scientists Clone SARS-CoV-2 Genome with Quick Yeast-Based Method (May 2020)
the-scientist.com/news-opinion/s…
7. Dr. Weiss's Magic Yeast

What this means is that instead of having to reconstruct the viral DNA first before introducing it into cells, you instead “put all these fragments in the yeast and it magically puts them together,” says Weiss.
8. TAR, YAC & DNA

transformation associated recombination (TAR)

yeast artificial chromosome (YAC)

Data Not Available (DNA) Don't believe me? Ask WIV!
9. Amazing Recombination Capacity

“The capacity to recombine things to a YAC by themselves is just amazing,” says Jores.
10. In Vitro Ligation

Refers to this UTMB paper:

An Infectious cDNA Clone of SARS-CoV-2

cell.com/cell-host-micr…

Note: Pei Yong Shi and James Le Duc
11. Method from above UTMB paper

An Infectious cDNA Clone of SARS-CoV-2

cell.com/cell-host-micr…
12. One Week Is All It Takes

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

nature.com/articles/s4158…
13. Nice Clear Layman's Explanation (Thank God)

Researchers develop synthetic novel coronavirus clones

phys.org/news/2020-05-s…
14. Well illustrated background reading

Billy Recommends: More Yeast with your Whining!

Synthetic genome engineering forging new frontiers for wine yeast

tandfonline.com/doi/full/10.10…
15. Virus Like Particle Vaccine Manufacture from Yeast
(Illustrated Master's Thesis) 2020
digitalcommons.mtu.edu/cgi/viewconten…
16. Synthetic viruses—Anything new?

asks Volker Thiel in 2018

The generation of synthetic poxviruses as described by Evans and colleagues and cloning of synthetic DNA using TAR in yeast are illustrated

journals.plos.org/plospathogens/…
17. Yeast-based synthetic genomics platform to genetically reconstruct RNA viruses exist.

How much were they used by Chinese Researchers in the years leading up to the pandemic is an interesting research project.
18. WIV Research (2018) - Yeast

Advances and challenges in enveloped virus-like particle (VLP)-based vaccines

Shiyu Dai, Hualin Wang, Fei Deng

State Key laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan

immunologyresearchjournal.com/public/article…
19. Time for a break!

Unroll the Yeast is Red @threadreaderapp
20. Revenge of Yeast
a WIV Thesis that @TheSeeker268 found recently is by Lei Ping Zeng (2017) & it mentions WIV's interest in yeast recombination methods

Reverse Genetic System of Bat SARS-like Coronaviruses & Function of ORFX
drive.google.com/drive/folders/…
21. Zeng Translated
(by @TheSeeker268 & @franciscodeasis )
docs.google.com/document/d/1uZ…
Only 2 references to yeast recombination but obviously on their radar back in 2017.
22. Across ocean & back in time to Wizard of UNC

Thanks Jim Haslam @jhas5

Yeast Based Small Molecule Screen for Inhibitors of SARS-CoV

journals.plos.org/plosone/articl…
23. I am getting so sick of Yeast!
I will just dump yeast infected papers here for others to vomit over
Yeast for virus research (2017) Richard Yuqi Zhao
ncbi.nlm.nih.gov/pmc/articles/P…
24. Enough Yeast!
2007: Saccharomyces cerevisiae: a versatile eukaryotic system in virology
…robialcellfactories.biomedcentral.com/articles/10.11…
2010: A High-Throughput Approach for Studying Virus Replication in Yeast
…rentprotocols.onlinelibrary.wiley.com/doi/10.1002/97…
25. I will finish with yeast until i get more yeastinspiration

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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 24
1. Panama Canal in the News

Historical Perspective

On the many workers' lives lost trying to build it:

Who were they and where did they come from?
2. First Attempt (French)

In the 1880s, the French tried but failed to build a Panama Canal.

They finally gave up in 1889, after years of fighting a recalcitrant landscape, ferocious disease and spiralling costs

AND the deaths of 22,000 workers! .

archive.ph/8FqKiImage
3. From Suez to Panama

Ferdinand de Lesseps's success in building the Suez Canal in 1869 was utterly derailed by the abysmal failure of his subsequent effort to build the Panama Canal due to disease.

Bankruptcy!

800 thousand people had invested their savings in the project! Image
Read 26 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 19
1. Russian Biolab Claims: A Controversial Topic

Good luck using your critical thinking skills to seperate wheat from chaff so that you can decide what is propaganda & what is fact.

Of course the easy options are to dismiss everything as lies or to swallow everything as fact.
2. What I recommend

If you are interested in a balanced analysis of the Russian claims and all the detailed evidence presented by Kirillov before his assassination in Moscow, as well as relted topics, then I highly recommend you plough through those threads at your leisure.
3. Documents & Russian claims on Projects UP-4 (avian flu) Flu-Flyway (wild ducks & bird flu), P-781 (bat coronaviruses), UP-8 (CCHF, Leptospirosis Hanta viruses):

Read 15 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 15
1. 'CONSPIRACY OF SILENCE"

Pentagon was told 6 times that Covid was lab leak at start of pandemic – but buried the truth, ex-intel official says.

Story now published with intriguing detail by @imogen_braddick after we informed her of John Myers Tweet

thesun.co.uk/news/32257526/… x.com/BillyBostickso…
2. John Myers said that:

he "knows with absolute certainty" that top US military chiefs were told Covid had leaked from a lab in Wuhan.

"the Joint Chiefs of Staff were briefed in October 2019 - more than five years ago - about a new virus that had leaked from a lab in China"
3. Claims

This was briefed in Oct& Nov 2019 as a lab leak.

It was in the intelligence. We briefed it. It was accepted. I briefed it numerous times about a viral outbreak and that it was from a lab.

"Over the course of late Nov & Dec, it came up 6 or 7 times in briefings.
Read 15 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 12
🧵📸 We have now solved the Pangolin Riddle!

This is BIG so sit tight!

1. WIV did have Pangolins in 2019 (we now have proof)

2. WIV isolated Pangolin/HKU4/MERS like Covs with Furin Sites in 2019, just before the pandemic!
3. Important Note

If you have not done so already, please read the linked thread above before continuing, it will make much more sense if you do so.
4. GX Pangolins at WIV in 2019!

From the Horse's Mouth in 2024!

"In 2019 & 2020, 86 anal swab samples & 80 serum samples were collected from Guangxi pangolins"

"Pangolin samples were transported & stored at Wuhan Institute of Virology, CAS"

cell.com/cell/fulltext/…Image
Read 53 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 11
Missing Documents from EHA & Peter Daszak

He hid all WeChat comms with WIV & Zhengli Shi from @COVIDSelect

However, EHA provided the #DOJ subpoena with tens of thousands of unredacted comms stretching back a decade.

AND where are these OIG audits:

#DHHS
@USGAO
@NIH
@USAID
1. @COVIDSelect @mitch_benzine @RepBradWenstrup

Have much more unreleased information

@COVIDSelect @mitch_benzine @RepBradWenstrup 2. DOJ Audit

1. "DOJ subpoenaed EcoHealth’s communications (inlcuding WeChat) with, at least, Dr. Shi. This subpoena included both Dr. Shi’s official WIV email address & her Hotmail"

2. “DoJ subpoena for genetic sequences, docs — almost complete”

nypost.com/2024/12/02/us-…
Read 9 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 11
Pangolin Riddle!

MpCoV-GX Isolated in 2017 said YiGang Tong

BUT ZLS & Yi GangTong in 2023 said:

"MpCoV-GX was isolated in 2019"

SARS-CoV-2-Related Virus from Malayan Pangolin

pmc.ncbi.nlm.nih.gov/articles/PMC99…

Accession data says submission date is 8 FEB 2020

ncbi.nlm.nih.gov/nuccore/MT0403… x.com/BillyBostickso…
2. Double Confirmed

Yi Gang Tong confrimed again the 2017 isolation date for MpCov-GX.

He experimented with it, but did not write a paper, and they did not upload the sequence until 8th Feb 2022, just after the beginning of the SARS-COV-2 pandemic

3. Why is this significant?

Yi Gang Tong stated in a paper received on 22 Feb 2020:

"no suspected infections were found in those having close contacts with pangolins"

"Our pangolin coronavirus isolate was routinely cultured in BSL 2 FACILITIES"

Read 20 tweets

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