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Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Jun 10, 2021 • 25 tweets • 18 min read • Read on X
Scrolly
1. The Yeast is Red and Many are Dead Thread
Sub Yeast Thread 1
2. via @ydeigin and @franciscodeasis
it’s a very disturbing paper if its findings are verified: not only SARS2 and RaTG13 have signs of genetic manipulation in yeast but SARS1 too.
WIV is known to have eyed yeast reverse genetic systems with interest (see attached WIV thesis
3. The Author has published quite a few interesting papers with 1870 citations.
scholar.google.com/citations?user…
One on the Association between influenza vaccination rates and SARS-CoV-2 outbreak infection rates in OECD countries
papers.ssrn.com/sol3/papers.cf…
4. Random Yeast Papers - WIV
Construction and Rescue of a Functional Synthetic Baculovirus
pubs.acs.org/doi/10.1021/ac…
5. Honours in Yeast (08/2020) - Yeast Virus Cookbook
Every Tweet is one Tab Less ;)
In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline
honours.eu/publications/i…
and pdf:
ncbi.nlm.nih.gov/pmc/articles/P…
6. Yeast containing SARS-COV- 2
“The exciting thing about the yeast is that . . . it’s fast,” says expert Susan Weiss
Scientists Clone SARS-CoV-2 Genome with Quick Yeast-Based Method (May 2020)
the-scientist.com/news-opinion/s…
7. Dr. Weiss's Magic Yeast

What this means is that instead of having to reconstruct the viral DNA first before introducing it into cells, you instead “put all these fragments in the yeast and it magically puts them together,” says Weiss.
8. TAR, YAC & DNA

transformation associated recombination (TAR)

yeast artificial chromosome (YAC)

Data Not Available (DNA) Don't believe me? Ask WIV!
9. Amazing Recombination Capacity

“The capacity to recombine things to a YAC by themselves is just amazing,” says Jores.
10. In Vitro Ligation

Refers to this UTMB paper:

An Infectious cDNA Clone of SARS-CoV-2

cell.com/cell-host-micr…

Note: Pei Yong Shi and James Le Duc
11. Method from above UTMB paper

An Infectious cDNA Clone of SARS-CoV-2

cell.com/cell-host-micr…
12. One Week Is All It Takes

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

nature.com/articles/s4158…
13. Nice Clear Layman's Explanation (Thank God)

Researchers develop synthetic novel coronavirus clones

phys.org/news/2020-05-s…
14. Well illustrated background reading

Billy Recommends: More Yeast with your Whining!

Synthetic genome engineering forging new frontiers for wine yeast

tandfonline.com/doi/full/10.10…
15. Virus Like Particle Vaccine Manufacture from Yeast
(Illustrated Master's Thesis) 2020
digitalcommons.mtu.edu/cgi/viewconten…
16. Synthetic viruses—Anything new?

asks Volker Thiel in 2018

The generation of synthetic poxviruses as described by Evans and colleagues and cloning of synthetic DNA using TAR in yeast are illustrated

journals.plos.org/plospathogens/…
17. Yeast-based synthetic genomics platform to genetically reconstruct RNA viruses exist.

How much were they used by Chinese Researchers in the years leading up to the pandemic is an interesting research project.
18. WIV Research (2018) - Yeast

Advances and challenges in enveloped virus-like particle (VLP)-based vaccines

Shiyu Dai, Hualin Wang, Fei Deng

State Key laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan

immunologyresearchjournal.com/public/article…
19. Time for a break!

Unroll the Yeast is Red @threadreaderapp
20. Revenge of Yeast
a WIV Thesis that @TheSeeker268 found recently is by Lei Ping Zeng (2017) & it mentions WIV's interest in yeast recombination methods

Reverse Genetic System of Bat SARS-like Coronaviruses & Function of ORFX
drive.google.com/drive/folders/…
21. Zeng Translated
(by @TheSeeker268 & @franciscodeasis )
docs.google.com/document/d/1uZ…
Only 2 references to yeast recombination but obviously on their radar back in 2017.
22. Across ocean & back in time to Wizard of UNC

Thanks Jim Haslam @jhas5

Yeast Based Small Molecule Screen for Inhibitors of SARS-CoV

journals.plos.org/plosone/articl…
23. I am getting so sick of Yeast!
I will just dump yeast infected papers here for others to vomit over
Yeast for virus research (2017) Richard Yuqi Zhao
ncbi.nlm.nih.gov/pmc/articles/P…
24. Enough Yeast!
2007: Saccharomyces cerevisiae: a versatile eukaryotic system in virology
…robialcellfactories.biomedcentral.com/articles/10.11…
2010: A High-Throughput Approach for Studying Virus Replication in Yeast
…rentprotocols.onlinelibrary.wiley.com/doi/10.1002/97…
25. I will finish with yeast until i get more yeastinspiration

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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 29
If you see a snake today..🐍

1. Based on the Parsi calendar conversion and the text of the Persian Mar-Nāmeh (THE BOOK FOR TAKING OMENS FROM SNAKES), seeing a snake today means you should not look at it, otherwise you will soon have a cause to be sorry.
2. Rule 7 cruelly says:

"If you see a snake on the day of Merdad (Amerdid) do not look at it, (or otherwise) you will soon have a cause to be sorry"

According to the Parsees, today is Roj Amardad (also spelled Merdad or Amerdāt).
3. If you see it, how can you not look at it?

The Book for Taking Omens from Snakes (30 Nov 1892)

By President-Surgeon-Major G. Waters.

"The custom of taking omens from snakes seems to be
very ancient. The people of antiquity associated with the snake various peculiar ideas"
Read 23 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 28
1. The morning breaks on human backs

Across the centuries & the tracks,
Where bone & muscle give their all,
To answer to the master's call.
And in the pocket, pouch, or hand,
A secret comfort rules the land.
A cheap escape from sweat & pain,
To dull the mind or spike the brain.
2. In London’s fog, the engine roars

And laborers crowd through palace doors,
To drown the shift in burning gin.
While in the slums, for just a penny,
The laudanum drops are shared by many,
To soothe the cough, the cramp, the tear,
And quiet the children crying there.
3. Across the sea, the jungle steams

Where coolies chased their broken dreams.
The shovel bites, the fever burns,
Until the evening sun returns.
Then in the bunkhouse, dark & small,
Sweet smoke rises on the wall;
The opium pipe is lit & blessed,
A pain-free haze, a perfect rest!
Read 11 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 27
1. Summarizing "Fauci's prequel" is not difficult!

Tony's Diary: The Prequel via @SenRandPaul

paul.senate.gov/wp-content/upl… x.com/BillyBostickso…
2. @_whitneywebb revealed just about everything

@_whitneywebb 3. Another short summary from 2020

Read 6 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 26
1. A shout out to all the clever and free apps that we have used for 6 years, without which we would be still in the dark

@threadreaderapp @ProtonMail @archiveis @OpenAlex_org @TheLensOrg @Gemini_Notebook @TranslateYandex @TransIateGoogIe @ResearchGate @ZENODO_ORG @arxiv @github
2. More apps used

@YouTube
@Vimeo
@rumblevideo
Google Patents
@waybackmachine
Justpasteit
@googledrive
@Gemini
@grok

Some Journals

@CellPressNews
@JAMA_current
@Nature
@NEJM
@PLOS
@embojournal

@VirologyJ
@biorxivpreprint
@medrxivpreprintpubmed.ncbi.nlm.nih.gov
@YouTube @Vimeo @rumblevideo @waybackmachine @googledrive @Gemini @grok @CellPressNews @JAMA_current @Nature @NEJM @PLOS @embojournal @VirologyJ @biorxivpreprint @medrxivpreprint 3. One big one I forgot...

@X

That made a vast difference, helping us coordinate our work through chat groups and share our work and keep the public and journalists and U.S. government organizations and commissions informed of the latest updates.
Read 4 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 15
1. Ball, Hung, Drawn and Quartered for Truth

Did ye sing, John Ball,
as they pulled you apart?
A poet speaking for us all
from England’s tattered heart.
What a price, John Ball,
for singing to us all.
When hope came as a crime
in simple rhythm and rhyme.
2. He who sings a blackbird’s song
to a starved & battered throng
with a message to stand strong
would never last long.
So we’ll sing to John Ball
for the helpless & hopeless,
the voiceless, forgotten,
buried beneath the mansions
of Lords; misbegotten
mortar, stones & chandeliers
3. the scarlet grave goods
of England’s preening peers.
We’ll sing in simple rhymes
and hope for better times,
when those barons’ grandchildren,
so viscous, reptilian,
are thrown from their seat
in ignominious defeat.
Read 8 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 12
🧵Explanation Thread

Here is my attempt at a simple summary of what @JasperFarlake & @dr_louwen claim (July 2026)

1. In early 2020, Chinese scientists published the first big paper on the COVID virus using fluid samples taken from patients’ lungs in Wuhan in December 2019.
2. They uploaded the raw sequencing data to public databases so others could check their work, which is great.



@JasperFarlake & @dr_louwen went back & carefully re-examined that raw data

They say they found several strange things that don’t look right.zenodo.org/records/213119…
@JasperFarlake @dr_louwen 3. You can see their findings in the readme.md text which you can see if you download their zip file and extract it.

or here directly on their @github repository

github.com/jasper-cmyk/PR…
Read 27 tweets

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