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Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Jun 10, 2021 • 25 tweets • 18 min read • Read on X
Scrolly
1. The Yeast is Red and Many are Dead Thread
Sub Yeast Thread 1
2. via @ydeigin and @franciscodeasis
it’s a very disturbing paper if its findings are verified: not only SARS2 and RaTG13 have signs of genetic manipulation in yeast but SARS1 too.
WIV is known to have eyed yeast reverse genetic systems with interest (see attached WIV thesis
3. The Author has published quite a few interesting papers with 1870 citations.
scholar.google.com/citations?user…
One on the Association between influenza vaccination rates and SARS-CoV-2 outbreak infection rates in OECD countries
papers.ssrn.com/sol3/papers.cf…
4. Random Yeast Papers - WIV
Construction and Rescue of a Functional Synthetic Baculovirus
pubs.acs.org/doi/10.1021/ac…
5. Honours in Yeast (08/2020) - Yeast Virus Cookbook
Every Tweet is one Tab Less ;)
In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline
honours.eu/publications/i…
and pdf:
ncbi.nlm.nih.gov/pmc/articles/P…
6. Yeast containing SARS-COV- 2
“The exciting thing about the yeast is that . . . it’s fast,” says expert Susan Weiss
Scientists Clone SARS-CoV-2 Genome with Quick Yeast-Based Method (May 2020)
the-scientist.com/news-opinion/s…
7. Dr. Weiss's Magic Yeast

What this means is that instead of having to reconstruct the viral DNA first before introducing it into cells, you instead “put all these fragments in the yeast and it magically puts them together,” says Weiss.
8. TAR, YAC & DNA

transformation associated recombination (TAR)

yeast artificial chromosome (YAC)

Data Not Available (DNA) Don't believe me? Ask WIV!
9. Amazing Recombination Capacity

“The capacity to recombine things to a YAC by themselves is just amazing,” says Jores.
10. In Vitro Ligation

Refers to this UTMB paper:

An Infectious cDNA Clone of SARS-CoV-2

cell.com/cell-host-micr…

Note: Pei Yong Shi and James Le Duc
11. Method from above UTMB paper

An Infectious cDNA Clone of SARS-CoV-2

cell.com/cell-host-micr…
12. One Week Is All It Takes

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

nature.com/articles/s4158…
13. Nice Clear Layman's Explanation (Thank God)

Researchers develop synthetic novel coronavirus clones

phys.org/news/2020-05-s…
14. Well illustrated background reading

Billy Recommends: More Yeast with your Whining!

Synthetic genome engineering forging new frontiers for wine yeast

tandfonline.com/doi/full/10.10…
15. Virus Like Particle Vaccine Manufacture from Yeast
(Illustrated Master's Thesis) 2020
digitalcommons.mtu.edu/cgi/viewconten…
16. Synthetic viruses—Anything new?

asks Volker Thiel in 2018

The generation of synthetic poxviruses as described by Evans and colleagues and cloning of synthetic DNA using TAR in yeast are illustrated

journals.plos.org/plospathogens/…
17. Yeast-based synthetic genomics platform to genetically reconstruct RNA viruses exist.

How much were they used by Chinese Researchers in the years leading up to the pandemic is an interesting research project.
18. WIV Research (2018) - Yeast

Advances and challenges in enveloped virus-like particle (VLP)-based vaccines

Shiyu Dai, Hualin Wang, Fei Deng

State Key laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan

immunologyresearchjournal.com/public/article…
19. Time for a break!

Unroll the Yeast is Red @threadreaderapp
20. Revenge of Yeast
a WIV Thesis that @TheSeeker268 found recently is by Lei Ping Zeng (2017) & it mentions WIV's interest in yeast recombination methods

Reverse Genetic System of Bat SARS-like Coronaviruses & Function of ORFX
drive.google.com/drive/folders/…
21. Zeng Translated
(by @TheSeeker268 & @franciscodeasis )
docs.google.com/document/d/1uZ…
Only 2 references to yeast recombination but obviously on their radar back in 2017.
22. Across ocean & back in time to Wizard of UNC

Thanks Jim Haslam @jhas5

Yeast Based Small Molecule Screen for Inhibitors of SARS-CoV

journals.plos.org/plosone/articl…
23. I am getting so sick of Yeast!
I will just dump yeast infected papers here for others to vomit over
Yeast for virus research (2017) Richard Yuqi Zhao
ncbi.nlm.nih.gov/pmc/articles/P…
24. Enough Yeast!
2007: Saccharomyces cerevisiae: a versatile eukaryotic system in virology
…robialcellfactories.biomedcentral.com/articles/10.11…
2010: A High-Throughput Approach for Studying Virus Replication in Yeast
…rentprotocols.onlinelibrary.wiley.com/doi/10.1002/97…
25. I will finish with yeast until i get more yeastinspiration

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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 26
@breakfast_dogs @gadboit @NLink247 1. Insect cell baculovirus cell cultures become contaminated with insect genomic material.
2. Replication process can lead to presence of viral DNA & cellular components in culture medium.
3. This LEADS to contamination of viral product with baculovirus & insect cell-derived DNA.
@breakfast_dogs @gadboit @NLink247 Contamination

4. Expression of SC-2 Spike RBD Using the Insect Cell-Baculovirus System

pmc.ncbi.nlm.nih.gov/articles/PMC97…

5. Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture

pmc.ncbi.nlm.nih.gov/articles/PMC71…

6. insect cell lines
sciencedirect.com/science/articl…
@breakfast_dogs @gadboit @NLink247 Did WIV use insect cell systems with SARS-C0V-2?

7. Insect cell baculovirus expression systems

x.com/BillyBostickso…

8. DENG Fei, Chief Investigator in Research Group on Viruses Resources at WIV engineered baculovirus-insect cell expression systems.

buzzchronicles.com/b/all/17652/
Read 13 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 22
1. Kadlec awaiting confirmation for his new role

2. Kadlec's report references pangolins 76 times

bush.tamu.edu/wp-content/upl…

3. Time to read my pangolin coronavirus report?

researchgate.net/publication/39…

4. DRASTIC research cited
@gdemaneuf @ydeigin @BillyBostickson @C_Small_ x.com/BoDeepest/stat…
1. the article linked is quite good, more comments and analysis will be posted here soon

city-journal.org/article/robert…Image
2. Possible lab manipulations

"2) insertion of a pangolin virus recombinant spike or the spike’s RBD protein that includes a sequence for an FCS and an integrin-binding protein" Image
Read 76 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 9
Pressure is needed on people like @realannapaulina @RepLuna & @GOPoversight team as their mandate may dissolve in August.

They did Kennedy, but they are supposed to do UFO files & CoVid-19 origins before they dissolve.

We also need to know about the subpoenas sent by @RandPaul
What was promised and demanded (for the record)

oversight.house.gov/release/rep-lu…

oversight.house.gov/release/chairm…
If you are a concerned US citizen please directly contact the people listed here:

oversight.house.gov/subcommittee/t…
Read 8 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 1
🧵Rev. Billy's Lab Leak Cover-Up Sermon

1. Brethren, hear me now, for we stand at the precipice of a great & grievous iniquity!

The world has been ensnared by a monstrous conspiracy, a vile machination spun by a clique of scientists, experts, media scribes & state overlords!
🧵2. Let us never forget!

Those who, in their arrogance, have sought to veil the truth of the COVID pestilence!

And I say to you today, that this is no mere error, but a deliberate, diabolical plot to obscure the origins of a plague that has scourged the nations!
🧵3. The Vocabulary of Deception

Let us expose this falsehood, this house of deceit

& the duties of the vigilant in these perilous times!

Oh, what a wretched lexicon these deceivers employ! Their words are cloaked in the garb of science, yet they reek of falsehood & treachery!
Read 23 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 29
GUESS WHO SAID THIS?

"During shooting in Thailand, I was shocked by Daszak’s fast & loose with facts, his refusal to acknowledge his conflict of interest,& his denial of his gain-of-function res in collaboration with Wuhan lab"
You guessed right, i hope?

@janeqiuchina

She continued:

"I was equally shocked by Daszak’s constant self-promotion and how effective it was." Image
@janeqiuchina About the film:

"Blame: Bats, Politics & a Planet Out of Balance"

"Christian Frei seemed entranced by hero worshipping, apparently having lost all sense of objectivity & critical judgment. I felt strongly then, as I do now, that the film was a blatant piece of propaganda" Image
Read 5 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 28
Sebastian Castellio (1551)

To seek truth & to utter what one believes to be true can never be a crime. No one must be forced to accept a conviction

Conviction is free

One of the first proponents of religious toleration, freedom of conscience & thought

gospeltruth.net/zweig/heresy_c…Image
One of the great forgotten men of the Reformation.

One of the greatest minds in Geneva, a brilliant scholar and thinker.

Sebastian Castellio published what has been called "the first manifesto in favor of toleration"

web.archive.org/web/2005030616…
The Plague in Geneva

At the same time, he was the only minister in Geneva during Calvin’s day to risk his life to assist the sick during the Black Plague.

The friend of Truth obeys not the multitude, but the Truth.”

evangelicalarminians.org/sebastian-cast…
Read 11 tweets

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