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Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Jun 10, 2021 • 25 tweets • 18 min read • Read on X
Scrolly
1. The Yeast is Red and Many are Dead Thread
Sub Yeast Thread 1
2. via @ydeigin and @franciscodeasis
it’s a very disturbing paper if its findings are verified: not only SARS2 and RaTG13 have signs of genetic manipulation in yeast but SARS1 too.
WIV is known to have eyed yeast reverse genetic systems with interest (see attached WIV thesis
3. The Author has published quite a few interesting papers with 1870 citations.
scholar.google.com/citations?user…
One on the Association between influenza vaccination rates and SARS-CoV-2 outbreak infection rates in OECD countries
papers.ssrn.com/sol3/papers.cf…
4. Random Yeast Papers - WIV
Construction and Rescue of a Functional Synthetic Baculovirus
pubs.acs.org/doi/10.1021/ac…
5. Honours in Yeast (08/2020) - Yeast Virus Cookbook
Every Tweet is one Tab Less ;)
In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline
honours.eu/publications/i…
and pdf:
ncbi.nlm.nih.gov/pmc/articles/P…
6. Yeast containing SARS-COV- 2
“The exciting thing about the yeast is that . . . it’s fast,” says expert Susan Weiss
Scientists Clone SARS-CoV-2 Genome with Quick Yeast-Based Method (May 2020)
the-scientist.com/news-opinion/s…
7. Dr. Weiss's Magic Yeast

What this means is that instead of having to reconstruct the viral DNA first before introducing it into cells, you instead “put all these fragments in the yeast and it magically puts them together,” says Weiss.
8. TAR, YAC & DNA

transformation associated recombination (TAR)

yeast artificial chromosome (YAC)

Data Not Available (DNA) Don't believe me? Ask WIV!
9. Amazing Recombination Capacity

“The capacity to recombine things to a YAC by themselves is just amazing,” says Jores.
10. In Vitro Ligation

Refers to this UTMB paper:

An Infectious cDNA Clone of SARS-CoV-2

cell.com/cell-host-micr…

Note: Pei Yong Shi and James Le Duc
11. Method from above UTMB paper

An Infectious cDNA Clone of SARS-CoV-2

cell.com/cell-host-micr…
12. One Week Is All It Takes

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

nature.com/articles/s4158…
13. Nice Clear Layman's Explanation (Thank God)

Researchers develop synthetic novel coronavirus clones

phys.org/news/2020-05-s…
14. Well illustrated background reading

Billy Recommends: More Yeast with your Whining!

Synthetic genome engineering forging new frontiers for wine yeast

tandfonline.com/doi/full/10.10…
15. Virus Like Particle Vaccine Manufacture from Yeast
(Illustrated Master's Thesis) 2020
digitalcommons.mtu.edu/cgi/viewconten…
16. Synthetic viruses—Anything new?

asks Volker Thiel in 2018

The generation of synthetic poxviruses as described by Evans and colleagues and cloning of synthetic DNA using TAR in yeast are illustrated

journals.plos.org/plospathogens/…
17. Yeast-based synthetic genomics platform to genetically reconstruct RNA viruses exist.

How much were they used by Chinese Researchers in the years leading up to the pandemic is an interesting research project.
18. WIV Research (2018) - Yeast

Advances and challenges in enveloped virus-like particle (VLP)-based vaccines

Shiyu Dai, Hualin Wang, Fei Deng

State Key laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan

immunologyresearchjournal.com/public/article…
19. Time for a break!

Unroll the Yeast is Red @threadreaderapp
20. Revenge of Yeast
a WIV Thesis that @TheSeeker268 found recently is by Lei Ping Zeng (2017) & it mentions WIV's interest in yeast recombination methods

Reverse Genetic System of Bat SARS-like Coronaviruses & Function of ORFX
drive.google.com/drive/folders/…
21. Zeng Translated
(by @TheSeeker268 & @franciscodeasis )
docs.google.com/document/d/1uZ…
Only 2 references to yeast recombination but obviously on their radar back in 2017.
22. Across ocean & back in time to Wizard of UNC

Thanks Jim Haslam @jhas5

Yeast Based Small Molecule Screen for Inhibitors of SARS-CoV

journals.plos.org/plosone/articl…
23. I am getting so sick of Yeast!
I will just dump yeast infected papers here for others to vomit over
Yeast for virus research (2017) Richard Yuqi Zhao
ncbi.nlm.nih.gov/pmc/articles/P…
24. Enough Yeast!
2007: Saccharomyces cerevisiae: a versatile eukaryotic system in virology
…robialcellfactories.biomedcentral.com/articles/10.11…
2010: A High-Throughput Approach for Studying Virus Replication in Yeast
…rentprotocols.onlinelibrary.wiley.com/doi/10.1002/97…
25. I will finish with yeast until i get more yeastinspiration

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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jan 29
@Ticklicker56 @angie_rasmussen @Dissenting2020 @DrStrangeLovett @Rebecca21951651 @All_New_to_This @VaughnMises @R_H_Ebright @Florin_Uncovers @ban_epp_gofroc @MJnanostretch @Muller_Lab @SolidEvidence @ydeigin @ciukzwil @tgof137 @StavaRune @VBruttel @FondueMean @tom335363 @BioSRP @stevenemassey @jbloom_lab @BallouxFrancois @Ayjchan @mattwridley Not sure what they have done since Burgdorfer's "swiss agent" experiments, apart from building tick moats & annoying locals

bitterrootstar.com/2013/01/about-…

Dr. Rasmussen can tell us or she can ask Dr. Trout

hcn.org/articles/in-mo…
@Ticklicker56 @angie_rasmussen @Dissenting2020 @DrStrangeLovett @Rebecca21951651 @All_New_to_This @VaughnMises @R_H_Ebright @Florin_Uncovers @ban_epp_gofroc @MJnanostretch @Muller_Lab @SolidEvidence @ydeigin @ciukzwil @tgof137 @StavaRune @VBruttel @FondueMean @tom335363 @BioSRP @stevenemassey @jbloom_lab @BallouxFrancois @Ayjchan @mattwridley RML expertise in biodefense inlcudes tick & flea agents, so I doubt she would want to discuss how many ticks they have or what they inject them with.

federalregister.gov/documents/2004…

In the bad old days they injected them with yellow fever & other nasties for dispersal by planes.
@Ticklicker56 @angie_rasmussen @Dissenting2020 @DrStrangeLovett @Rebecca21951651 @All_New_to_This @VaughnMises @R_H_Ebright @Florin_Uncovers @ban_epp_gofroc @MJnanostretch @Muller_Lab @SolidEvidence @ydeigin @ciukzwil @tgof137 @StavaRune @VBruttel @FondueMean @tom335363 @BioSRP @stevenemassey @jbloom_lab @BallouxFrancois @Ayjchan @mattwridley Also work of Jeffrey M. Grabowski

Biology of Vector-Borne Viruses Section, Laboratory of Virology, RML

Work on tick-borne pathogens at the Virology Lab at RML is funded by the Intramural Research Program, NIAID, NIH

Not for long, I think! @DrJBhattacharya
Read 15 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jan 20
1. Latest Victim of Flo Debarre's "Doxxng" Crusade

Is ME!

I got a disturbing & threatening email, mentioning:

"room for negotiation about the name"

"1st story is often the one that sticks, even if it's not accurate..your chance to negotiate terms"

"continue over Signal" Image
2. Le Monde

The reporter she is working with, William Audureau @Willvs , seems a principled journalist and did not know of her nefarious doxxing activities against me and other members of DRASTIC and our supporters in Academia, of which he dissaproves. Image
@Willvs 3. Best way to deal with bullies is to expose them!

I thus emailed CNRS, Cyber Security Office & the Minister of the Interior to complain

presidence.secretariat@cnrs.fr
presse@cnrs.fr ,
b.retailleau@senat .fr @BrunoRetailleau
contact@cecyf.fr
fraude-bretic@interieur.gouv.fr Image
Image
Read 12 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jan 2
An unexpected result in a UK Porton Down Study

"Participant 12" (maybe a soldier at Aldershot). after infection with SC2, over time experienced a substantial number of mutations suggestive of rapid evolution.

Yet he was healthy & not immunocompetent?

genomemedicine.biomedcentral.com/articles/10.11…
They don't consider the possibility of quasispecies viral swarms as a possible factor in Participant 12?

Quasispecies implications for virus persistence (Domingo, 1999)

pmc.ncbi.nlm.nih.gov/articles/PMC71…

Quasispecies theory & emerging viruses
(Sardanyes, 2024)

nature.com/articles/s4429… Image
Overview of Viral Quasispecies

sciencedirect.com/topics/immunol…

Quite good Wikipedia Page

en.wikipedia.org/wiki/Viral_qua…

Viral quasispecies (Andino & Domingo, 2015)

sciencedirect.com/science/articl…

Historical Perspective on the Quasispecies Concept (Domingo, 2021)

annualreviews.org/docserver/full…
Read 19 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 29, 2024
🧵DTIC MIL WUHAN/COVID-19 DOCUMENTS

Just dumping these here like Santa!

Maybe you will find something of interest?

Maybe a lump of coal or a potato!

1. Novel Coronavirus 2019 (COVID-19): Q&A on Global Implications and Responses (May 14, 2020)

apps.dtic.mil/sti/pdfs/AD117…
2. Award Number: W81XWH-11-2-0148 (2013)

WIV, Wuhan University, Le Duc and GNL mentioned

TITLE: National Biocontainment Training Center

PRINCIPAL INVESTIGATOR: James LeDuc, PhD

University of Texas Medical Branch at Galveston

apps.dtic.mil/sti/pdfs/ADA61…Image
Image
Image
3. Features, Evaluation, & Treatment of Coronavirus

Tedious Paper

March 1, 2021.

apps.dtic.mil/sti/pdfs/AD112…
Read 14 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 24, 2024
1. Panama Canal in the News

Historical Perspective

On the many workers' lives lost trying to build it:

Who were they and where did they come from?
2. First Attempt (French)

In the 1880s, the French tried but failed to build a Panama Canal.

They finally gave up in 1889, after years of fighting a recalcitrant landscape, ferocious disease and spiralling costs

AND the deaths of 22,000 workers! .

archive.ph/8FqKiImage
3. From Suez to Panama

Ferdinand de Lesseps's success in building the Suez Canal in 1869 was utterly derailed by the abysmal failure of his subsequent effort to build the Panama Canal due to disease.

Bankruptcy!

800 thousand people had invested their savings in the project! Image
Read 26 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Dec 19, 2024
1. Russian Biolab Claims: A Controversial Topic

Good luck using your critical thinking skills to seperate wheat from chaff so that you can decide what is propaganda & what is fact.

Of course the easy options are to dismiss everything as lies or to swallow everything as fact.
2. What I recommend

If you are interested in a balanced analysis of the Russian claims and all the detailed evidence presented by Kirillov before his assassination in Moscow, as well as relted topics, then I highly recommend you plough through those threads at your leisure.
3. Documents & Russian claims on Projects UP-4 (avian flu) Flu-Flyway (wild ducks & bird flu), P-781 (bat coronaviruses), UP-8 (CCHF, Leptospirosis Hanta viruses):

Read 15 tweets

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