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Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Jun 10, 2021 • 25 tweets • 18 min read • Read on X
Scrolly
1. The Yeast is Red and Many are Dead Thread
Sub Yeast Thread 1
2. via @ydeigin and @franciscodeasis
it’s a very disturbing paper if its findings are verified: not only SARS2 and RaTG13 have signs of genetic manipulation in yeast but SARS1 too.
WIV is known to have eyed yeast reverse genetic systems with interest (see attached WIV thesis
3. The Author has published quite a few interesting papers with 1870 citations.
scholar.google.com/citations?user…
One on the Association between influenza vaccination rates and SARS-CoV-2 outbreak infection rates in OECD countries
papers.ssrn.com/sol3/papers.cf…
4. Random Yeast Papers - WIV
Construction and Rescue of a Functional Synthetic Baculovirus
pubs.acs.org/doi/10.1021/ac…
5. Honours in Yeast (08/2020) - Yeast Virus Cookbook
Every Tweet is one Tab Less ;)
In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline
honours.eu/publications/i…
and pdf:
ncbi.nlm.nih.gov/pmc/articles/P…
6. Yeast containing SARS-COV- 2
“The exciting thing about the yeast is that . . . it’s fast,” says expert Susan Weiss
Scientists Clone SARS-CoV-2 Genome with Quick Yeast-Based Method (May 2020)
the-scientist.com/news-opinion/s…
7. Dr. Weiss's Magic Yeast

What this means is that instead of having to reconstruct the viral DNA first before introducing it into cells, you instead “put all these fragments in the yeast and it magically puts them together,” says Weiss.
8. TAR, YAC & DNA

transformation associated recombination (TAR)

yeast artificial chromosome (YAC)

Data Not Available (DNA) Don't believe me? Ask WIV!
9. Amazing Recombination Capacity

“The capacity to recombine things to a YAC by themselves is just amazing,” says Jores.
10. In Vitro Ligation

Refers to this UTMB paper:

An Infectious cDNA Clone of SARS-CoV-2

cell.com/cell-host-micr…

Note: Pei Yong Shi and James Le Duc
11. Method from above UTMB paper

An Infectious cDNA Clone of SARS-CoV-2

cell.com/cell-host-micr…
12. One Week Is All It Takes

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

nature.com/articles/s4158…
13. Nice Clear Layman's Explanation (Thank God)

Researchers develop synthetic novel coronavirus clones

phys.org/news/2020-05-s…
14. Well illustrated background reading

Billy Recommends: More Yeast with your Whining!

Synthetic genome engineering forging new frontiers for wine yeast

tandfonline.com/doi/full/10.10…
15. Virus Like Particle Vaccine Manufacture from Yeast
(Illustrated Master's Thesis) 2020
digitalcommons.mtu.edu/cgi/viewconten…
16. Synthetic viruses—Anything new?

asks Volker Thiel in 2018

The generation of synthetic poxviruses as described by Evans and colleagues and cloning of synthetic DNA using TAR in yeast are illustrated

journals.plos.org/plospathogens/…
17. Yeast-based synthetic genomics platform to genetically reconstruct RNA viruses exist.

How much were they used by Chinese Researchers in the years leading up to the pandemic is an interesting research project.
18. WIV Research (2018) - Yeast

Advances and challenges in enveloped virus-like particle (VLP)-based vaccines

Shiyu Dai, Hualin Wang, Fei Deng

State Key laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan

immunologyresearchjournal.com/public/article…
19. Time for a break!

Unroll the Yeast is Red @threadreaderapp
20. Revenge of Yeast
a WIV Thesis that @TheSeeker268 found recently is by Lei Ping Zeng (2017) & it mentions WIV's interest in yeast recombination methods

Reverse Genetic System of Bat SARS-like Coronaviruses & Function of ORFX
drive.google.com/drive/folders/…
21. Zeng Translated
(by @TheSeeker268 & @franciscodeasis )
docs.google.com/document/d/1uZ…
Only 2 references to yeast recombination but obviously on their radar back in 2017.
22. Across ocean & back in time to Wizard of UNC

Thanks Jim Haslam @jhas5

Yeast Based Small Molecule Screen for Inhibitors of SARS-CoV

journals.plos.org/plosone/articl…
23. I am getting so sick of Yeast!
I will just dump yeast infected papers here for others to vomit over
Yeast for virus research (2017) Richard Yuqi Zhao
ncbi.nlm.nih.gov/pmc/articles/P…
24. Enough Yeast!
2007: Saccharomyces cerevisiae: a versatile eukaryotic system in virology
…robialcellfactories.biomedcentral.com/articles/10.11…
2010: A High-Throughput Approach for Studying Virus Replication in Yeast
…rentprotocols.onlinelibrary.wiley.com/doi/10.1002/97…
25. I will finish with yeast until i get more yeastinspiration

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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 29
GUESS WHO SAID THIS?

"During shooting in Thailand, I was shocked by Daszak’s fast & loose with facts, his refusal to acknowledge his conflict of interest,& his denial of his gain-of-function res in collaboration with Wuhan lab"
You guessed right, i hope?

@janeqiuchina

She continued:

"I was equally shocked by Daszak’s constant self-promotion and how effective it was." Image
@janeqiuchina About the film:

"Blame: Bats, Politics & a Planet Out of Balance"

"Christian Frei seemed entranced by hero worshipping, apparently having lost all sense of objectivity & critical judgment. I felt strongly then, as I do now, that the film was a blatant piece of propaganda" Image
Read 5 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 28
Sebastian Castellio (1551)

To seek truth & to utter what one believes to be true can never be a crime. No one must be forced to accept a conviction

Conviction is free

One of the first proponents of religious toleration, freedom of conscience & thought

gospeltruth.net/zweig/heresy_c…Image
One of the great forgotten men of the Reformation.

One of the greatest minds in Geneva, a brilliant scholar and thinker.

Sebastian Castellio published what has been called "the first manifesto in favor of toleration"

web.archive.org/web/2005030616…
The Plague in Geneva

At the same time, he was the only minister in Geneva during Calvin’s day to risk his life to assist the sick during the Black Plague.

The friend of Truth obeys not the multitude, but the Truth.”

evangelicalarminians.org/sebastian-cast…
Read 11 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 28
🧵Latest SAGO Report

1. A good summary by @mattwridley but let's look at some details from the report, available here:

cdn.who.int/media/docs/def… x.com/R_H_Ebright/st…
2, Conflicts of Interest and Bias

Summed up by @R_H_Ebright

@R_H_Ebright 3. SAGO history and our struggle (January 2022)

Read 20 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 24
Bloody Hell, it's worse than even I imagined in my darkest nightmares about #scamdemia.

Time to spend some of this tax money on local electronics, biological, ecological, IT, optical, chemistry, engineering & physics labs run by local amateurs for the benefit of our communities?
DRASTIC MEASURES NEEDED

Finally #scamdemia is waking up to the putrid stench of education for profit, ruined lives and academic credential inflation?

Read 5 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 9
Short 🧵on Primer & PCR Test issues (Pangolin Covs)

1. The key issue with pangolins and Pcovs is that:

It was discovered that the standard PCR tests used in 2020, and often still used, failed to actually detect many of the betacoronaviruses generously hosted by the pangolins.
2. Unless

The samples are retested with bespoke primers & more accurate PCR tests available now,

We will not know exactly whIch Pangolin (or bat) coronaviruses were hosted by Pangolins at WIV & elsewhere pre-pandemic.

see Nga et al., 2022 for details

frontiersin.org/journals/publi…Image
3. It is unlikely that anyone will do that now😒

However, "they" (I will name who they are later) should, and perhaps in the future, may retest the stored samples at WIV, IPB, SCAU AND GIABR, to clarify the question of which coronaviruses were present in their pangolin samples.
Read 22 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jun 4
1. Tip of the Riceberg?

Not to mention wheat, barley and maize!

Read the disturbing criminal compaint here:

wlns.com/wp-content/upl… x.com/danaparish/sta…
2. May have been for research and commercial purposes, but they were caught bang to rights.

"LIU initially stated that he did not know what the materials were and that someone must have put them into his bag" Image
3. When asked why someone would put them into his bag, LIU stated that he did not know, and that maybe he had accidentally put the materials there. After further questioning, LIU acknowledged that the materials were different strains of the pathogen Fusarium graminearum
Read 17 tweets

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