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Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Jun 10, 2021 • 25 tweets • 18 min read • Read on X
Scrolly
1. The Yeast is Red and Many are Dead Thread
Sub Yeast Thread 1
2. via @ydeigin and @franciscodeasis
it’s a very disturbing paper if its findings are verified: not only SARS2 and RaTG13 have signs of genetic manipulation in yeast but SARS1 too.
WIV is known to have eyed yeast reverse genetic systems with interest (see attached WIV thesis
3. The Author has published quite a few interesting papers with 1870 citations.
scholar.google.com/citations?user…
One on the Association between influenza vaccination rates and SARS-CoV-2 outbreak infection rates in OECD countries
papers.ssrn.com/sol3/papers.cf…
4. Random Yeast Papers - WIV
Construction and Rescue of a Functional Synthetic Baculovirus
pubs.acs.org/doi/10.1021/ac…
5. Honours in Yeast (08/2020) - Yeast Virus Cookbook
Every Tweet is one Tab Less ;)
In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline
honours.eu/publications/i…
and pdf:
ncbi.nlm.nih.gov/pmc/articles/P…
6. Yeast containing SARS-COV- 2
“The exciting thing about the yeast is that . . . it’s fast,” says expert Susan Weiss
Scientists Clone SARS-CoV-2 Genome with Quick Yeast-Based Method (May 2020)
the-scientist.com/news-opinion/s…
7. Dr. Weiss's Magic Yeast

What this means is that instead of having to reconstruct the viral DNA first before introducing it into cells, you instead “put all these fragments in the yeast and it magically puts them together,” says Weiss.
8. TAR, YAC & DNA

transformation associated recombination (TAR)

yeast artificial chromosome (YAC)

Data Not Available (DNA) Don't believe me? Ask WIV!
9. Amazing Recombination Capacity

“The capacity to recombine things to a YAC by themselves is just amazing,” says Jores.
10. In Vitro Ligation

Refers to this UTMB paper:

An Infectious cDNA Clone of SARS-CoV-2

cell.com/cell-host-micr…

Note: Pei Yong Shi and James Le Duc
11. Method from above UTMB paper

An Infectious cDNA Clone of SARS-CoV-2

cell.com/cell-host-micr…
12. One Week Is All It Takes

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

nature.com/articles/s4158…
13. Nice Clear Layman's Explanation (Thank God)

Researchers develop synthetic novel coronavirus clones

phys.org/news/2020-05-s…
14. Well illustrated background reading

Billy Recommends: More Yeast with your Whining!

Synthetic genome engineering forging new frontiers for wine yeast

tandfonline.com/doi/full/10.10…
15. Virus Like Particle Vaccine Manufacture from Yeast
(Illustrated Master's Thesis) 2020
digitalcommons.mtu.edu/cgi/viewconten…
16. Synthetic viruses—Anything new?

asks Volker Thiel in 2018

The generation of synthetic poxviruses as described by Evans and colleagues and cloning of synthetic DNA using TAR in yeast are illustrated

journals.plos.org/plospathogens/…
17. Yeast-based synthetic genomics platform to genetically reconstruct RNA viruses exist.

How much were they used by Chinese Researchers in the years leading up to the pandemic is an interesting research project.
18. WIV Research (2018) - Yeast

Advances and challenges in enveloped virus-like particle (VLP)-based vaccines

Shiyu Dai, Hualin Wang, Fei Deng

State Key laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan

immunologyresearchjournal.com/public/article…
19. Time for a break!

Unroll the Yeast is Red @threadreaderapp
20. Revenge of Yeast
a WIV Thesis that @TheSeeker268 found recently is by Lei Ping Zeng (2017) & it mentions WIV's interest in yeast recombination methods

Reverse Genetic System of Bat SARS-like Coronaviruses & Function of ORFX
drive.google.com/drive/folders/…
21. Zeng Translated
(by @TheSeeker268 & @franciscodeasis )
docs.google.com/document/d/1uZ…
Only 2 references to yeast recombination but obviously on their radar back in 2017.
22. Across ocean & back in time to Wizard of UNC

Thanks Jim Haslam @jhas5

Yeast Based Small Molecule Screen for Inhibitors of SARS-CoV

journals.plos.org/plosone/articl…
23. I am getting so sick of Yeast!
I will just dump yeast infected papers here for others to vomit over
Yeast for virus research (2017) Richard Yuqi Zhao
ncbi.nlm.nih.gov/pmc/articles/P…
24. Enough Yeast!
2007: Saccharomyces cerevisiae: a versatile eukaryotic system in virology
…robialcellfactories.biomedcentral.com/articles/10.11…
2010: A High-Throughput Approach for Studying Virus Replication in Yeast
…rentprotocols.onlinelibrary.wiley.com/doi/10.1002/97…
25. I will finish with yeast until i get more yeastinspiration

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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Nov 14
🧵Long live the lab-leak hypothesis!

1. The long awaited 293 questions for Dr. Ralph Baric have finally been completed and shared! Long live the quest for truth! You are hereby invited to read the sharpest, most lethal 293-question dossier ever built.

researchgate.net/publication/39…
🧵Long live the quest for truth!

2. When that day finally comes & Baric raises his right hand, every word in there will be waiting for him


@RandPaul @RandPaulReview @TulsiGabbard
@SenJoniErnst @R_H_Ebright @jhas5 @ydeigin @gdemaneuf @mentju4 @emilyakopphsgac.senate.gov/media/reps/cha…
@RandPaul @RandPaulReview @TulsiGabbard @SenJoniErnst @R_H_Ebright @jhas5 @ydeigin @gdemaneuf @mentju4 @emilyakopp 🧵3. Watch the video explainer here

Courtesy of Google Notebook LM

vimeo.com/1136899679
Read 6 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Nov 12
✴️MICROPLASTICS IN YOUR BRAIN?

1. "These tiny fragments were far smaller than cells and even smaller than many viruses. Some were close to the size of DNA strands. Under high magnification, she noticed small hooked shapes with sharp points.
✴️2. OVERLOOKED

"She realized that the plastic in human brains was not only present, but surprisingly small. The fragments were so tiny that common diagnostic tools could not detect them. Pathologists had likely been overlooking them for years."

msn.com/en-us/health/o…
✴️3. NONE WERE FREE

"The two original samples showed a strong link between plastic particles and dementia. As Bearer expanded her work to ten more donated brains, she found plastic in every single one. None were free of synthetic fragments."
Read 23 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Nov 6
🧵1. In the interview with @danaparish

Redfield stresses that the evidence points to lab creation for a reusable, aerosolized vaccine vector, with US complicity in funding/tech.

He says he is fed up with the cover-ups and predicts that full intel release would confirm this.
🧵2. Proof?

Unfortunately, there is no direct proof provided by Redfield in this new interview, but he cites engineering features, early data, and classified information.
🧵3. Summary of Redfield's Statements

(on SARS-CoV-2 Origins & Cover-Up)

He claims SARS-CoV-2 originated from GOF research in a lab and emphasizes a significant US role alongside China.

He views this as a major biosecurity failure with ongoing suppression of transparency.
Read 21 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Oct 30
🧵1. Critique of preprint: SARS-COV-2 like FCS hype

Media article 1

dailymail.co.uk/health/article…

Media article 2

straitstimes.com/world/coronavi…
🧵2. The Authors State:

"Of note, the spike protein of this novel bat coronavirus possesses a functional FCS at the S1/S2 junction with a unique amino acid sequence motif (RDAR) that differs from that found in SARS-CoV-2 (RRAR) by only 1 amino acid."

biorxiv.org/content/10.110…
🧵3. Two fatal flaws: Overreach & Undermine

A. The authors of this study on the BRZ batCoV from Brazil unfortunately overreach themselves when they connect it to SARS-CoV-2 origins.

B. The FCS evidence actually undermines the flawed hypothesis of direct bat to human emergence!
Read 13 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Oct 25
🧵1. Honest question

Did you try to read my pangolin coronavirus report but gave up because it was too long or too complex?

If you didn't see it, link is in next tweet

Help is at hand!

Thanks to Google's pretty amazing "notebook lm"

A video explainer

notebooklm.google.com/notebook/1b843…
🧵2. Just in case..

The massive report is here:

researchgate.net/publication/39…
🧵3. An updated brief explanation on Medium

The Pangolin-Mediated Laboratory Origin of SARS-CoV-2

medium.com/p/the-pangolin…
Read 8 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Oct 4
"The molecular biology capabilities of WIV & the genome assessment are consistent with the hypothesis that SARS-CoV-2 was a lab-engineered virus that was part of a bank of chimeric viruses in Zheng-Li Shi's laboratory at WIV that escaped from containment"

usrtk.org/wp-content/upl…
2. Hypothetical Laboratory Origin of SARS-CoV-2

Between 2017 and 2019, WIV created full-length a infectious clone in pBAC-CMV using an unpublished bat Coronavirus genome as template (BatCoVX)

page 85
3. Hypothesis 3:

Between 2017 and 2019, WIV created chimeric Bat-CoV-X viruses using the pBACCMV-BCOVX backbone and swapping out key cassettes with other bat Coronaviruses (RBD, RBM, etc.) and adding additional features such as a furin cleavage site
Read 15 tweets

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