1/ 🧵How SURFACE CHARGE (zeta potential) on LNP DIRECTS WHERE in body it goes (LIVER then distribute, OR, DIRECTLY TO SPLEEN, or DIRECTLY TO HEART OR LUNGS) , AND WHY this explains multiple types of ADVERSE EVENTS, including the recent FATAL HEART STUDY that is being shared.
2/CHARGES: WATCH video (refresher for some): This video (Mr. Anderson) explains how positive (+), negative (-), and neutral (0) items interact. How even something WITH a + CHARGE can ATTACH to something NEUTRAL.
Trying to use basic explanations for all.
3/ LNP: My silly drawing. NOT ALL lipids will be discussed. WE are FOCUSING on CHARGE.
The RED backward S= negatively charged mod RNA (-). The GREEN plus (+) is POSITIVELY charged ionizable lipid. They stick together (better pic included). Also; Pfizer's doc: zeta= -3 mV.
4/ ONLY watch video if you know science. In the video/study, SCIENTISTS, took LNP w/ RNA (-) w/ pos. lipids (+) and injected into mice--different kinds--some w/ equal (-)/(+)=neutral, some more (+), some more (-), some VERY negative(--), and tracked
5/ WHERE in mouse's body LNP/RNA went, according to CHARGE. Zeta potential is complicated. It has factors: SIZE of LNP, Amount of positive (+) and negative (-) charges in it, surface charge (outer layer) in respect to pH, and other things. This is watered down, for simplicity.
6/ The scientists at the NANO company and OTHERS (not conspiracy theory, not my theory), FOUND that if they adjusted the RATIO of positive (+) to negative charges (-) inside the LIPID NANOPARTICLE, they found the LNP did NOT go to the LIVER and distribute, as initially thought.
7. The scientists found that, if the LNP has a surface charge that is NEUTRAL (ish), it goes to LIVER and distributes to other areas of the body, as seen in initial studies, and clinical trial (sans the DNA plasmids, yes?) This was with a zeta potential approx. - 3 millivolts
8/ BUT if the LNP had a higher (+) POSITIVE CHARGE, it did NOT go to the LIVER and distribute--it goes to LUNGS. IF it has slightly negative (-) charge, it goes to SPLEEN. If it has MORE NEGATIVE (--), it can LEAK into VASCULAR (HEART, vein, artery) and cause bad things.
9/ If the charge is positive (+), it is zipping right to the lungs. It does not matter if you aspirate. This is by injecting into the MUSCLE, not just IV. MUSCLE. It could cause injury in lung (PE?). If a little negative, it goes to spleen. If VERY negative, it goes to HEART.
10/ It can also cause (if very negative charge): CLOTS (binding to platelet factor 4--proven in AstraZeneca study), create a PROTEIN CORONA (the LNP will bind with albumin, other plasma proteins--diff thread)--possible injury, and express spike in endothelium of vascular.
11/ Now, let us look at the basic construction of the LNP in very simple terms for as many to understand as possible. LNP is made of cholesterol, DSPC, "unprotonated" (has not received a charge yet from H+) ionizable lipids, charged (+) lipids, PEG, and modRNA (-) charge.
12/ as seen above, the LNP is self-assembling (this is a term certain "people" are taking the wrong way--the LNP assembles in the LAB, because of charges and contents.) However, the companies must have measured the "charge" on the LNP, without DNA plasmids. They did this w/IVT.
13/ (this is old drawing, before it was shared that the DNA plasmids are in pieces. The purple circle in NEXT drawing is the DNA plasmid. The Red backward S is still modRNA, and green plus (+) is positively charged lipids.
First, it was thought that the LNP only had modRNA in it
14/ But instead of just modRNA, and lipids in mix, there is DNA plasmids in the mix when the LNP is forming. The measurement of the zeta potential, -3 mV was done using a process to make the LNP and modRNA using IVT (no plasmids). Hence, might not be accurate measured charge.
15/ Two things could have happened. You could have DNA plasmid pieces (excuse the plasmid circles as they represent the DNA plasmid) which are (-) surrounded by pos lipids (+) forming lipoplex--not part of RNA LNP structure. OR, DNA/RNA lipopolyplex (modRNA AND DNA inside). Now,
16/ The DNA polyplex by itself should have a more positive charge (going to the lungs!). And according to the study precision nano and others did, highly negative is going to vascular system, which would account, for what was described previously. This new study just came out.
17/ This study says: ". . VACCINE WAS NOT DETECTED IN THE MEDIASTINAL LYMPH NODES, SPLEEN, OR LIVER. VACCINE WAS DETECTED IN THE MYOCARDIUM IN A SUBSET OF PATIENTS VACCINATED WITHIN 30 DAYS OF DEATH."
nature.com/articles/s4154…
18/ "Serious adverse complications due to these vaccines are uncommon and may include anaphylactic reactions, myocarditis, pericarditis, myocardial infarction, cerebral sinus thrombosis, stroke, pulmonary embolism, neuropathies, and autoimmune hepatitis"
We know, from studies,
19/ That when you alter the ratio of (+) charged lipids to negative charged (-) modRNA, this would change where it goes in the body. The contamination of DNA plasmids MUST be CHANGING the ZETA potential and overall charge of the LNP, causing it, sometimes, to go to vascular/lung
20/ Another thing that could be happening, in addition to DNA plasmids changing the zeta potential (charge) is the LNP is breaking down during the freeze/thaw process, and parts can leak out, changing the overall charge as well. Dr. Ko and De, and others proved.
21/ Negative charges also cause clots (negatively charged liposomes) as shown above.
The introduction of the negatively charged DNA plasmids, at a rate of billions per dose, at 100 bp (base pair average, must be shifting zeta. It probably distributes UNEVENLY, which accounts
22/ for heterogeneity (differences) between batches and vials. Because it is not evenly distributed, there would be batch an vial variation. The FDA needs to investigate immediately, and need to immediately halt distribution.
The DNA plasmids are of significant concern.
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(you could still watch--it just might melt the brain or confuse)
A thread on the CpG motif contained within the DNA plasmid and what the immune system and other impacts it most likely is causing.
(multiple studies to back)
@kharaguchi
I was limited to 24 tweets in thread. If the DNA plasmids have entered the LNP, this means it is shifting the zeta in a negative direction. The calculations have been done assuming size differences and using two different formula sets.
Approximations using base pair average of 100 was used.
Some may contain far more DNA plasmids than others which would cause it to shift greater into the negative direction.
Because not everyone is having these effects, It must be the case that some the lipid nanoparticle have far more DNA plasmids in them than others which would account for the differences in charge. The lipid nanoparticle also vary in size which means, depending on some factors, so would zeta.
The opposite of what most would assume to be true is not.
The smaller the LNP, the stronger the charge.
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