Thread by @CyrilPedia

I love this paper. For decades immunologists fought over the role of antigen persistence in memory. It was impossible to settle: there is always the possibility of residual antigen, somewhere on an FDC

nature.com/articles/35036…

If you transfer cells, you might still be transferring antigen etc Ag specific memory cells are rare, so minimal antigen might matter.

So Maruryam et al decide to just flip the BCR after immunization

So who cares if Ag persists, the memory B cells wont see it

Because the CRE driver is Ifn inducible and leaky, the actual results are not perfect. But still, the basic concept was simple & elegant (from 2000).