Thread by @akulmehta

Proud to share our latest publication which shows how to synthesize a library of #glycopeptides at microgram scale in a parallel and cost effective manner published in @CellChemBiol
cell.com/cell-chemical-…
Collaboration between @theNCFG @NPohl_Group & @BoyceLab
#glycotime

The method is based on SPOT synthesis technique (classically used to produce non-glycosylated peptides), which we modified to help with special conditions required for glycoamino acids (which are more fragile). We also developed a biopsy sampling technique to monitor reactions.

Synthesis can be done without need for specialized equipment, in a chemical fume-hood with filter paper/cellulose (solid support), a glass food lock-lock type container and chemical reagents used in traditional FMOC peptide chemistry and other common lab equipment e.g. pipettes.

We prepared glycopeptides with several sequences (8-22mer) and containing O-, N-, and S-linked glycosides to create 2 independant libraries. We even incorporated non-natural amino acids (e.g. fluoro-prolines) and tags (e.g. fluorescein) to show the versatility of the technique.

We demonstrated how to handle and use the libraries produced for:
1. Microarrays - to probe antibodies
2. Enzymatic Reactions (on-array and in-solution) - e.g. ST6GalNAc1
3. Binding Affinity - e.g. VVL
4. Mass Spectrometry

We are already using this to produce more libraries.