To celebrate National Hispanic Heritage Month, I will highlight the work I do throughout Latin America.
Both sides of my family are from Mexico and I have a special affinity for the Yucatan Peninsula! Image
My first expedition was to Ecuador to field test our new multi-parallel real-time quantitative PCR. qPCR is more sensitive/specific than microscopy and also to test for cure and reinfection. Action shots of me in the jungle and Pacific coast. @AJTMH
ajtmh.org/docserver/full… ImageImageImageImage
Next is Argentina, this study used WGS to determine the decrease of vitamin B12 genes in children with Giardia, a potential pathway for growth delays in children. Pics of me in Northern Argentina. @bugbittentweets @a5hi5h @RebeccaJeun @a5hi5h
parasitesandvectors.biomedcentral.com/articles/10.11… ImageImageImage
Heading north, the next study was in rural Honduras. We associated Blastocystis infections with helminths. Also increasing Blastocystis burden with age and exposure. Pics of me and 2 amazing scientists I know @AnaSanchezPhD @mebottazzi ! @AJTMH @Naceanc
ajtmh.org/content/journa… ImageImageImageImage
The next stop is Guatemala, using our recombinant Strongyloides antigen ELISA, we detected over 11% strongyloidiasis prevalence. Work is pending publication. Pics of me and the beautiful Guatemalan landscape. ImageImageImage
The next Latin American country is Colombia. We investigated the similarities between children living in urban slums versus poor rural areas. Children infected with intestinal parasites although different environmental exposures. Preprint available for review. @patriciaebryan ImageImageImageImage
The next country is El Salvador, where we started an intestinal parasite impact on Chagas disease study. This collaboration with the University of South Carolina will look at immune polarization with parasite co-infections. Abstract to follow at ASTMH 2020 conference.
And to wrap up Hispanic Heritage Month, I return to Mexico, the country of my ancestors. I am working with @uady_mx processing environmental samples for parasites and how they impact people living in poverty. ImageImageImageImage
We are also starting research in Brazil. Overall the Laboratory of Human Parasasitology is heavily invested in Central/South America. We have translational research in 8 countries and continue to understand parasites, poverty, and disease.

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More from @Rojelio

18 Aug
An important reminder on the potenital catastrophe of disseminated strongyloidiasis. A key teaching point is we have 5 to 25% of people living in the USA infected with Strongyloides.
ajtmh.org/content/journa…
We detected 25% in people infected with HIV living in the DC, Maryland, and Virginia area.
tandfonline.com/doi/full/10.11…
We detected 4.2% strongyloidiasis in a convenience study of people in Washington D.C.
cambridge.org/core/journals/…
Read 11 tweets
24 Jun
Processing soil samples from the Southern USA. We use flotation, filtration, and concentration technique to isolate parasite DNA from dirt. @dwmccormick @BlackburnCrudo
We first weigh and dry the samples, making the sample easier to work with and also maximizing the yield of parasite DNA.
After drying, we divide the samples an take a dry weight.
Read 11 tweets
9 Jun
Our final print is out for Strongyloides stercoralis Infection in Solid Organ Transplant Patients Is Associated With Eosinophil Activation and Intestinal Inflammation: A Cross-sectional Study. Thanks to @EvaClarkMD @Strongylady @karlajbautista_ @a5hi5h
academic.oup.com/cid/advance-ar…
We detected increased eosinophils in those infected with Strongyloides and associated with increased Strongy specific IgG. Image
Strongyloides activated the innate immune response and activates the eosinophils to release their cytotoxic granule proteins. Also associated with increased Strongy IgG. Image
Read 6 tweets

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