To celebrate National Hispanic Heritage Month, I will highlight the work I do throughout Latin America.
Both sides of my family are from Mexico and I have a special affinity for the Yucatan Peninsula!
My first expedition was to Ecuador to field test our new multi-parallel real-time quantitative PCR. qPCR is more sensitive/specific than microscopy and also to test for cure and reinfection. Action shots of me in the jungle and Pacific coast. @AJTMH ajtmh.org/docserver/full…
Heading north, the next study was in rural Honduras. We associated Blastocystis infections with helminths. Also increasing Blastocystis burden with age and exposure. Pics of me and 2 amazing scientists I know @AnaSanchezPhD@mebottazzi ! @AJTMH@Naceanc ajtmh.org/content/journa…
The next stop is Guatemala, using our recombinant Strongyloides antigen ELISA, we detected over 11% strongyloidiasis prevalence. Work is pending publication. Pics of me and the beautiful Guatemalan landscape.
The next Latin American country is Colombia. We investigated the similarities between children living in urban slums versus poor rural areas. Children infected with intestinal parasites although different environmental exposures. Preprint available for review. @patriciaebryan
The next country is El Salvador, where we started an intestinal parasite impact on Chagas disease study. This collaboration with the University of South Carolina will look at immune polarization with parasite co-infections. Abstract to follow at ASTMH 2020 conference.
And to wrap up Hispanic Heritage Month, I return to Mexico, the country of my ancestors. I am working with @uady_mx processing environmental samples for parasites and how they impact people living in poverty.
We are also starting research in Brazil. Overall the Laboratory of Human Parasasitology is heavily invested in Central/South America. We have translational research in 8 countries and continue to understand parasites, poverty, and disease.
An important reminder on the potenital catastrophe of disseminated strongyloidiasis. A key teaching point is we have 5 to 25% of people living in the USA infected with Strongyloides. ajtmh.org/content/journa…
Processing soil samples from the Southern USA. We use flotation, filtration, and concentration technique to isolate parasite DNA from dirt. @dwmccormick@BlackburnCrudo
We first weigh and dry the samples, making the sample easier to work with and also maximizing the yield of parasite DNA.
After drying, we divide the samples an take a dry weight.
We detected increased eosinophils in those infected with Strongyloides and associated with increased Strongy specific IgG.
Strongyloides activated the innate immune response and activates the eosinophils to release their cytotoxic granule proteins. Also associated with increased Strongy IgG.