Anatomy of a real-time PCR or RT-PCR (PCR or RT-rPCR) curve. 
Two things to highlight using these stylised rPCR curves.
First.
The yellow arrows highlight threshold cycles.
These are the "results". The number from the point at which each curve crosses that arbitrary horizontal threshold are recorded by the lab and reported as "detected"
First.
The yellow arrows highlight threshold cycles.
These are the "results". The number from the point at which each curve crosses that arbitrary horizontal threshold are recorded by the lab and reported as "detected"
Second.
These values are *not* the same numbers as the TOTAL number of cycles that the rPCR is run for (you can read about cycles here if of interest virologydownunder.com/the-mechanics-…)
These values are *not* the same numbers as the TOTAL number of cycles that the rPCR is run for (you can read about cycles here if of interest virologydownunder.com/the-mechanics-…)
So in this version, I've taken away the text and just left the Ct arrows for the 4 detected and a problematic result (bad curve shape, late) that would need further examination (green), and added a red arrow. That arrow marks the TOTAL number of cycles-differs from the Cts, yeah?
Can I just clarify - this is a stylised *example* - it's specific Ct values aren't specifically meaningful or applicable to every run of rPCR ever!
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