2021 is off to a great start! I’m very excited to share my new study at @CellStemCell on using surface antigen-guided CRISPR screens to identify genes that regulate myeloid leukemia differentiation.

Here's my first tweetorial!
1. Here, we used two innovative genetic screen approaches, a cell surface-based genome-wide and single-cell (ECCITE-seq) CRISPR screens to identify regulators of AML differentiation.
2. We focused our efforts on the mRNA decay factor, ZFP36L2 which demonstrated a strong dependency in human myeloid leukemia cell lines (@CancerDepMap).
3. Knockout of ZFP36L2 resulted in several upregulated genes associated with myeloid differentiation. Together with ZFP36L2 eCLIP (thanks to @bio_eclipse), we found that this RNA-binding protein interacts with the 3’UTR of specific myeloid maturation mRNAs.
4. Moreover, we identified a selective enhancer nearby ZFP36L2 and perturbation of this enh-ZFP36L2 using CRISPRi led to changes in the differentiated state of AML cells.
5. Lastly, special thanks to @psmibert and Eleni for the technical help on using ECCITE-seq! Also, this work would not be possible without the amazing team of @iannisaifantis1 and the #aifantislab crew!

• • •

Missing some Tweet in this thread? You can try to force a refresh

Keep Current with Eric Wang

Eric Wang Profile picture

Stay in touch and get notified when new unrolls are available from this author!

Read all threads

This Thread may be Removed Anytime!


Twitter may remove this content at anytime! Save it as PDF for later use!

Try unrolling a thread yourself!

how to unroll video
  1. Follow @ThreadReaderApp to mention us!

  2. From a Twitter thread mention us with a keyword "unroll"
@threadreaderapp unroll

Practice here first or read more on our help page!

Did Thread Reader help you today?

Support us! We are indie developers!

This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3/month or $30/year) and get exclusive features!

Become Premium

Too expensive? Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal Become our Patreon

Thank you for your support!

Follow Us on Twitter!