Our paper looking at the long-term maturation of cortical organoids is out! rdcu.be/cfDQt #organoids #brainorganoids
TL;DR - human cortical organoids start to resemble postnatal cortical development after 250-300 days of culturing
TL;DR - human cortical organoids start to resemble postnatal cortical development after 250-300 days of culturing
This work was a great collaboration between @GeschwindLab @PascaStanford @gracexiao99 and @HuguenardLab. Thank you to all coauthors @SeJinYoon3, Sthephan Tran, @MakinsonLab, @jimena_andersen, @FreddyMValencia and Steve Horvath
In this project, we generated human cortical organoids from 6 human iPSC lines and grew them for up to 694(!) days
We found that based on an unbiased genome-wide approach taking into account all the changes in gene expression across time, cortical organoids start to look like postnatal brain after 250-300 days of culturing
Seeing a strong similarity between organoids and the developing brain, we compared the biological processes between these two systems and found that many of the processes that occur during cortical development are preserved in the organoids
We found that RNA editing which is dynamically regulated during development, is also partially preserved in these cortical organoids
To explore prenatal to postnatal transition in the organoids we looked at gene changes that occur around birth such as NMDA receptors NR2A/B that switch around birth in the developing cortex and found the same switch in the organoids at around 250 days.
Looking at the expression of genes associated with many neurodevelopmental and neurodegenerative disorders we found that these genes clustered into distinct groups based on when they were expressed.
At least one of these clusters in each disorder peaked in expression after 250 days of differentiation suggesting that longer differentiation will be needed to study these genes.
We provide a web tool – GECO – which allows for a detailed comparison of gene expression between cortical organoids and the developing cortex
labs.dgsom.ucla.edu/geschwind/file…
labs.dgsom.ucla.edu/geschwind/file…
This work was made possible by funding from @CIRM @NIMHgov @BBRFoundation @AutismScienceFd @NSF @nyscf @czi
• • •
Missing some Tweet in this thread? You can try to
force a refresh
