3/
Paraphrasing: "My dream was to become an engineer but somehow I outperfomed myself so I was selected to study biology at ECNU for some reason" "You're kidding!"
Paraphrasing: "My dream was to become an engineer but somehow I outperfomed myself so I was selected to study biology at ECNU for some reason" "You're kidding!"
4/
"I was very good at cloning!"
"I was very good at cloning!"
5/
"Huge contribution by Duke Medical School in Singapore"
Goes back and forth between Australia and Singapore; Duke twice a year
BSL-3 and 4 (Australia), can work on animals of all different sizes
"Huge contribution by Duke Medical School in Singapore"
Goes back and forth between Australia and Singapore; Duke twice a year
BSL-3 and 4 (Australia), can work on animals of all different sizes
6/
"This building was built to fight FMDV but the farmers were worried about importing live virus... so we have to rely on our collaborators in Thailand and Vietnam to do the vaccine trials and things like that"
"This building was built to fight FMDV but the farmers were worried about importing live virus... so we have to rely on our collaborators in Thailand and Vietnam to do the vaccine trials and things like that"
7/
en.wikipedia.org/wiki/Australia…
upgraded to BSL4 for Hendra... BSL4 equals chemical showers and spacesuits... 3 shower minimum... box within a box
In other countries SARS is BSL3, in Australia BSL4
Nipah! Nipah! SARS! MERS!
"We can work on any pathogens"
en.wikipedia.org/wiki/Australia…
upgraded to BSL4 for Hendra... BSL4 equals chemical showers and spacesuits... 3 shower minimum... box within a box
In other countries SARS is BSL3, in Australia BSL4
Nipah! Nipah! SARS! MERS!
"We can work on any pathogens"
8/
Can work on animals at any level from 2-4
Whole building is PC4 physical containment but BSLs different
unique animal facility: microbats to horses, "everything except giraffe and elephant, could even do tigers and lions"
Can work on animals at any level from 2-4
Whole building is PC4 physical containment but BSLs different
unique animal facility: microbats to horses, "everything except giraffe and elephant, could even do tigers and lions"
9/
Full-time job 4 HEPA engineers
Box within a box
Table tennis and gym
"Controversy in the US not safe we're all human we all make mistakes, but if you come inside you understand how safe it is"
Full-time job 4 HEPA engineers
Box within a box
Table tennis and gym
"Controversy in the US not safe we're all human we all make mistakes, but if you come inside you understand how safe it is"
10/
So you're DR BAT... BATPACK!
"My life is fate"
So you're DR BAT... BATPACK!
"My life is fate"
11/
SARS was BIG
I was selected for 8 member team for SARS1... I know the language and politics and culture...
2nd field trip paid off... closest virus we can get... more than 10 years collaborating with Shi... puzzled not being able to isolate virus...
SARS was BIG
I was selected for 8 member team for SARS1... I know the language and politics and culture...
2nd field trip paid off... closest virus we can get... more than 10 years collaborating with Shi... puzzled not being able to isolate virus...
12/
everybody tried... Gary Cranberry (?) from LW lab went to Wuhan... optimised the isolation procedure... "talking about getting the infectious virus"
"As a virologist eventually you have to prove and isolate the real virus"
everybody tried... Gary Cranberry (?) from LW lab went to Wuhan... optimised the isolation procedure... "talking about getting the infectious virus"
"As a virologist eventually you have to prove and isolate the real virus"
13/
PCR doesn't prove infectious virus is there? Sequence alone is suggestive
bat poo? live virus or full length genome plus lots of serology, sequence alone not enough
FULL TILT INTO BATS, lots of people, lots of viruses
PCR doesn't prove infectious virus is there? Sequence alone is suggestive
bat poo? live virus or full length genome plus lots of serology, sequence alone not enough
FULL TILT INTO BATS, lots of people, lots of viruses
14/
very rare fatal bat (?) tick viruses...
Nipah
HERPES IS EVERYWHERE
How about that coronavirus huh?
very rare fatal bat (?) tick viruses...
Nipah
HERPES IS EVERYWHERE
How about that coronavirus huh?
15/
c 29:30
Do bats get infected even? Yes, but not reproducibly
CDC have really good breeding colony (bats)
Australian/Chinese group sequenced 2 bat genomes
large small bats... common ancestor? genome says yes
c 29:30
Do bats get infected even? Yes, but not reproducibly
CDC have really good breeding colony (bats)
Australian/Chinese group sequenced 2 bat genomes
large small bats... common ancestor? genome says yes
16/
c 32:30
DNA damage repair
Bats very rarely have tumours, long lifespan, lots of viruses don't get sick
Sourcing bats is not easy (for animal trials)
c 32:30
DNA damage repair
Bats very rarely have tumours, long lifespan, lots of viruses don't get sick
Sourcing bats is not easy (for animal trials)
17/
c 36:00 @BillyBostickson
"CDC have a breeding colony but we're working on that"
We take fresh urine drops on a plastic sheet and isolate five real viruses from one drop
"We're doing cell level and whole bats as well"
c 36:00 @BillyBostickson
"CDC have a breeding colony but we're working on that"
We take fresh urine drops on a plastic sheet and isolate five real viruses from one drop
"We're doing cell level and whole bats as well"
18/
c 42:00 @BillyBostickson
"So right now before you have a colony you have to get bats? They're captured from Australia, from other places as well..."
"Microbats are much harder to handle in a BSL4 environment.. we have done two challenge experiments for SARS..."
c 42:00 @BillyBostickson
"So right now before you have a colony you have to get bats? They're captured from Australia, from other places as well..."
"Microbats are much harder to handle in a BSL4 environment.. we have done two challenge experiments for SARS..."
19/
c 42:30
but not again because keeping microbats alive in BSL4 is a challenge... trained them to eat worms from (wiggled) forceps twice a day
Chinese collaborators can give only 5 microlitres of blood per individual bat
Fruitbat? YOU'RE A BIG BAT
c 42:30
but not again because keeping microbats alive in BSL4 is a challenge... trained them to eat worms from (wiggled) forceps twice a day
Chinese collaborators can give only 5 microlitres of blood per individual bat
Fruitbat? YOU'RE A BIG BAT
20/
c 44:00 @BillyBostickson
"We have to build this cages large enough for social activity but small enough so they feel they cannot fly"
But what if not flying biases the experiments? No practical solution
Ticks and mosquitoes might transmit viruses to them
c 44:00 @BillyBostickson
"We have to build this cages large enough for social activity but small enough so they feel they cannot fly"
But what if not flying biases the experiments? No practical solution
Ticks and mosquitoes might transmit viruses to them
21/
c 47:00 @BillyBostickson
antibody and cellular responses, large grant in Singapore
early stages 5 year program (2014)
still hoping learn from bats translate to livestock with transgenic or humans gene therapy
c 47:00 @BillyBostickson
antibody and cellular responses, large grant in Singapore
early stages 5 year program (2014)
still hoping learn from bats translate to livestock with transgenic or humans gene therapy
22/
c 48:45
hope... to learn things to make us more resistant
49:00
MERS
"I am biased [think it came from bats]"
Dr Shi main corresponding author, ACE2 receptor bat virus
"Camel could be one of the animals donating..."
c 48:45
hope... to learn things to make us more resistant
49:00
MERS
"I am biased [think it came from bats]"
Dr Shi main corresponding author, ACE2 receptor bat virus
"Camel could be one of the animals donating..."
23/
c 49:30
first (related virus to MERS??) was from Hong Kong
collaboration all over the world
DABS ON PCR. DO SEROLOGY TESTS!
c 49:30
first (related virus to MERS??) was from Hong Kong
collaboration all over the world
DABS ON PCR. DO SEROLOGY TESTS!
24/
"I joined the lab end of 1990... the theme of fate"
c 54:00
weekly bat piss/shit sheets
c 56:00
mice models for Hendra ferret models for Henipa
"I joined the lab end of 1990... the theme of fate"
c 54:00
weekly bat piss/shit sheets
c 56:00
mice models for Hendra ferret models for Henipa
25/
c 57:00
"It's a ONE HEALTH approach"
Vincent Münsters (???) group at Rocky Mountain MERS vaccine Fort Collins
DO YOU LOVE THIS?
"If I had a choice in Shanghai in '78..."
c 57:00
"It's a ONE HEALTH approach"
Vincent Münsters (???) group at Rocky Mountain MERS vaccine Fort Collins
DO YOU LOVE THIS?
"If I had a choice in Shanghai in '78..."
26/
c 1:02:00
BAT ACTION
37 drops of really good quality urine
"This smell of urine... is that them??"
c 1:02:00
BAT ACTION
37 drops of really good quality urine
"This smell of urine... is that them??"
27/
c 1:03:00 @BillyBostickson @uacjess @WhereIsYanLing @gdemaneuf @Daoyu15 @LynnFynn3 @still_a_nerd
"So we're always joking about, you know, you can just hopefully not drop something into your eye or mouth then get an inoculation you know." "Hmmm, yeah."
c 1:03:00 @BillyBostickson @uacjess @WhereIsYanLing @gdemaneuf @Daoyu15 @LynnFynn3 @still_a_nerd
"So we're always joking about, you know, you can just hopefully not drop something into your eye or mouth then get an inoculation you know." "Hmmm, yeah."
28/
c 1:03:45
SUPERSHEDDER BATS FLYING OVERHEAD PISSING AND SHITTING
"Beautiful" "It's perfect" "Heh, heh, heh, heh"
c 1:03:45
SUPERSHEDDER BATS FLYING OVERHEAD PISSING AND SHITTING
"Beautiful" "It's perfect" "Heh, heh, heh, heh"
29/
c 1:04:00
"Bats in Western cultures is dark, evil but in Chinese means fortune, my scientific fortune took off since I studied bat virus." "Makes sense."
c 1:04:00
"Bats in Western cultures is dark, evil but in Chinese means fortune, my scientific fortune took off since I studied bat virus." "Makes sense."
30/
31/
NB the audio is better on the youtube video
c 6:00
WOW the BIG GUYS are coming
NB the audio is better on the youtube video
c 6:00
WOW the BIG GUYS are coming
32/
NOT IN MY EYES MATE! NOT IN MY MOUTH!
NOT IN MY EYES MATE! NOT IN MY MOUTH!
33/
Improved audio:
4:30 "They are very smelly animals. Yeah. So we're always joking about yeah, know, just hopefully they don't drop something to, into your eye or mouth and get an inoculation you know."
Improved audio:
4:30 "They are very smelly animals. Yeah. So we're always joking about yeah, know, just hopefully they don't drop something to, into your eye or mouth and get an inoculation you know."
35/
The bat pack
The bat virology team, located at the Australian Animal Health Laboratory (AAHL) in Geelong Victoria, includes 25 scientists dedicated to unravelling the mysteries associated with bats and the relationship they have with the viruses they carry.
The bat pack
The bat virology team, located at the Australian Animal Health Laboratory (AAHL) in Geelong Victoria, includes 25 scientists dedicated to unravelling the mysteries associated with bats and the relationship they have with the viruses they carry.
36/
Dr Linfa Wang: identifying and characterising new animal viruses @BillyBostickson
web.archive.org/web/2014122312…
Dr Linfa Wang: identifying and characterising new animal viruses @BillyBostickson
web.archive.org/web/2014122312…
37/
"Dr Wang has published more than 300 scientific publications, many in highly recognised journals including Science, Nature Lancet Infectious Diseases and the Proceedings of the National Academy of Sciences (PNAS)."
"Dr Wang has published more than 300 scientific publications, many in highly recognised journals including Science, Nature Lancet Infectious Diseases and the Proceedings of the National Academy of Sciences (PNAS)."
38/
How odd @uacjess
"As of 2020 he has authored or contributed to over 300 scientific papers"
en.wikipedia.org/wiki/Peter_Das…
How odd @uacjess
"As of 2020 he has authored or contributed to over 300 scientific papers"
en.wikipedia.org/wiki/Peter_Das…
39/
Dr Wang holds a number of other honorary positions and memberships, including:
Honorary Professor, Wuhan Institute of Virology, Chinese Academy of Science, 2005–present
Honorary Professor, East China Normal University, 2011–present
Dr Wang holds a number of other honorary positions and memberships, including:
Honorary Professor, Wuhan Institute of Virology, Chinese Academy of Science, 2005–present
Honorary Professor, East China Normal University, 2011–present
unroll plox 😘 @threadreaderapp
• • •
Missing some Tweet in this thread? You can try to
force a refresh
