Here is a short #Tweetorial on #HowIReadThisPaper which aims to address whether combinatorial treatment with temozolamide and riluzole is effective against glioblastoma
doi.org/10.3171/2019.1…
@MedicsAcademy
#NeuroOncology
doi.org/10.3171/2019.1…
@MedicsAcademy
#NeuroOncology
MGMT might make you think of millennial electropop, but read on to find out what else it should make the keen #NeuroOncologist think of.
open.spotify.com/track/1jJci4qx…
open.spotify.com/track/1jJci4qx…
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Glutamatergic signalling promotes growth in glioblastomas. Riluzole is a glutamate receptor 1 inhibitor shown to suppress GBM growth. Temozolamide is an established chemotherapeutic agent for the treatment GBM. However, the combined effects of these drugs are unknown.
Glutamatergic signalling promotes growth in glioblastomas. Riluzole is a glutamate receptor 1 inhibitor shown to suppress GBM growth. Temozolamide is an established chemotherapeutic agent for the treatment GBM. However, the combined effects of these drugs are unknown.
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Human and murine cell lines were treated with TMZ, riluzole or a combination of both, testing the effect of riluzole on MGMT which is a DNA repair enzyme giving chemoresistance to TMZ. Reducing MGMT helps TMZ work better.
Human and murine cell lines were treated with TMZ, riluzole or a combination of both, testing the effect of riluzole on MGMT which is a DNA repair enzyme giving chemoresistance to TMZ. Reducing MGMT helps TMZ work better.
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In addition, the authors also tested the efficacy of combinatorial riluzole and temozolamide treatment in an orthotopic mouse allograft model of MGMT positive GBM using C57BL/6 J mice.
In addition, the authors also tested the efficacy of combinatorial riluzole and temozolamide treatment in an orthotopic mouse allograft model of MGMT positive GBM using C57BL/6 J mice.
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Riluzole displays a significant time-dependent and dose dependent growth inhibition on all GBM cell lines
Riluzole displays a significant time-dependent and dose dependent growth inhibition on all GBM cell lines
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Riluzole enhanced the anti-tumour effects of TMZ synergistically in MGMT positive but not in MGMT negative cell lines
Riluzole enhanced the anti-tumour effects of TMZ synergistically in MGMT positive but not in MGMT negative cell lines
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Riluzole suppressed MGMT mRNA expression and suppressed TMZ induced growth MGMT upregulation (p<0.01)
Riluzole suppressed MGMT mRNA expression and suppressed TMZ induced growth MGMT upregulation (p<0.01)
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Combination of riluzole/TMZ significantly suppressed tumour growth in the intracranial MGMT-positive GBM model (p<0.05)
Combination of riluzole/TMZ significantly suppressed tumour growth in the intracranial MGMT-positive GBM model (p<0.05)
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So what’s the bottom line?
Riluzole attenuates TMZ-induced MGMT upregulation and enhances the anti-tumour effect of TMZ in MGMT-positive GBMs
So what’s the bottom line?
Riluzole attenuates TMZ-induced MGMT upregulation and enhances the anti-tumour effect of TMZ in MGMT-positive GBMs
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Combinatorial Riluzole with TMZ is a potential novel therapeutic regimen in MGMT positive GBM tumours.
Combinatorial Riluzole with TMZ is a potential novel therapeutic regimen in MGMT positive GBM tumours.
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