Thread by @Bin_YANG_Optics

We just updated our preprint, reporting a novel single-objective #lightsheet design that is capable of imaging large samples, with a large imaging volume, uncompromized image resolution and speed, and multi-view imaging. (1/9)

doi.org/10.1101/2020.0…

Thanks to @DrLachie, now we have a visual abstract which explains how the microscope works. First half of the movie below. (2/9)

Second half of the visual abstract by @DrLachie (3/9)

The FWHMs of the PSF are 450nm laterally and 2 μm axially. The imaging volume is a few mm * 800 μm * 300 μm. This is achieved through a combination of using #KingSnout objective (@AndrewGYork @amsikking ) for #remotefocusing and Light-Sheet Stabilized Stage Scanning (#LS3). (4/9)

We demonstrated the combination of large imaging volume and high spatial resolution by imaging a whole #zebrafish larvae. Images are obtained from single 3D stack acquisitions and do not require stitching, corresponding to an imaging volume of 3000 μm * 800 μm * 300 μm. (5/9)

We also imaged the development of #Drosophila fly egg chambers (@georgewanpeng @kornberg_t ) for 3 hours. The imaging volume is 3000 μm* 800 μm* 180 μm. (6/9)

Our microscope is compatible with multi-well imaging. We mounted 9 embryos in a dish and imaged them sequentially (at 4.5 minutes per round) for a total of 8 hours. This mounting strategy is easily scalable, allowing high throughput screening. (7/9)
#development

You can find related materials at github.com/royerlab/daxi, where we provide a list of parts used to build the microscope, #Python code for optical simulation, device control and the #MicroManager acquisition script, etc. (8/9)

Many thanks for all co-authors for their contribution to this work. Much appreciated! @Merlin_Lange @amsikking @_ahmetcansolak @ShruthiVijayKu2 @georgewanpeng @liilii_tweet, Matthew N. McCarroll, @DrLachie @RetoPaul @kornberg_t @AndrewGYork @loicaroyer (9/9)