New preprint showing a #SARSCoV2 nucleocapsid variant that impacts antigen test performance. One sample just didn't want to play ball with the Quidel SARS Antigen FIA, of #pac12 and #big10 fame. So we sequenced it...
medrxiv.org/content/10.110…
medrxiv.org/content/10.110…
Using additional clinical specimens and recombinant nucleocapsid, we mapped to D399N. Looks like a ~1000-fold effect on assay performance. Doesn't affect Quidel QuickVue or BinaxNOW. This is a rare mutation, occurring in 0.02% global genomes. But variants are everywhere.
big thanks to @GAPerchetti for initially pulling this thread and Jon Reed and Quynh Phung in the lab for recombinant work, Michelle Lin for data viz, Dr. Lori Bourassa for coordination and @DrKimHarmon for the initial tests.
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