1/ I’m super excited to post our recent findings on the mucosal immune response to COVID-19 vaccines. medrxiv.org/content/10.110…
2/ In this manuscript, we show that the IgG anti-Spike response in the saliva to mRNA/mRNA and Ad/mRNA vaccination regimes reflects the serum IgG response. However, we also observe a saliva IgA response that is associated with secretory component, particularly after one dose.
3/ This secretory chain associated Ab response was most evident in subjects with high IgA levels in their saliva. We measured this by using an anti-secretory component secondary detection reagent. Thanks to @florian_krammer for the protocol.
4/ Unlike the IgG response which increases after dose 2, the sIgA response to vaccination is highest after the 1st dose. We hypothesize that high IgG levels induced by dose 1 may prevent delivery of antigen to the mucosa during dose 2.
5/ We also show that heterologous Ad/mRNA Vx induces a similar salivary antibody response as mRNA/mRNA. Good news for Canadians, many of whom have had this regime. Thanks to volunteers for contributing samples including LTC staff, @mattgallowaycbc and @cafreeland.
6/ Lastly, with help from @ImmunoFever we found that there was a modest level of nAb in the saliva. This may have the capacity to blunt transmission, at least against Wuhan SARS-CoV-2.
7/ Big question moving forward: Is there sufficient Ab in the saliva to prevent transmission of VOC? Working with @CoVaRR_Net we aim to find this out.
8/ Special thanks to @gingraslab1 for serum analysis, @AlainnaJJ for multivariate analysis, @COVIDimmunityTF / Sharon Strauss, and @CIHR_IRSC for funding us, and especially Gommerlab-ers @SalmaSheikhM_ , @baweletaisho, @mzuo_ and @OlgaLRojas2. FIN.
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