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Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Oct 22, 2021 • 17 tweets • 15 min read • Read on X
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1. Shining the Spotlight on Dr. Marion Koopmans

via @angoffinet and @uacjess

Principal Investigator

erasmusmc.nl/en/research/re…

"Erasmus MC is closely involved with EcoHealth Alliance"

erasmusmc.nl/en/research/de…

(under collaboration)

2. Don't forget to send that email to SAGO@who.int

with subject:

“Public comments on SAGO members” by 27 October 2021

3. Marion Koopman's COIs summarised

4. Wuhan Lab Leak "Debunked"

De KNAW. (2020). KNAW-symposium: Het nieuwe coronavirus - Marion Koopmans [YouTube Video]. Retrieved from

Minute 14:00
5. You Tube Q and A

Dr Marion Koopmans on the WHO Investigation of the Origins of the Pandemic



"we found not a grain of evidence for a lab leak theory"

Minute: 2:10
6. PR for WIV

Marion Koopmans doing PR for WIV:

"It's It's certainly not a big big thing"

WHO team scientist: Wuhan lab workers did fall sick in autumn of 2019

nbcnews.com/nightly-news/v…

7. Insert And Backbone?

"we need to talk about the backbone too, not just the insert"



Unredacting Koopman's email may have been an error
8. "Ask Marion" Part 1

(English Translation) 11/06/2021 by @hommel_b

janbhommel.nl/ask-marion-1-e…
9. "Ask Marion" Part 2

(English Translation) 20/06/202 by @hommel_b

janbhommel.nl/ask-marion-2-e…

Archived: archive.ph/Gczf3
10. "Ask Marion" Part 3

(English Translation) 05/07/2021 by @Hommel_bee

Two names are missing under this Lancet letter, while one would expect them, Marion Koopmans and Ron Fouchier...

janbhommel.nl/ask-marion-3-e…

Archived: archive.ph/ajgMx
11. Why did Koopmans not sign the Lancet Letter?

On Feb 7, 2020, Daszak sent an email to Colwell with a list of people they want to approach to sign the statement, including Osterhaus, Koopmans and Haagmans. Haagmans signed it. Koopmans will not.

The question remains; why not?
12. "Ask Marion" Part 4

20/07/2021 by @Hommel_be

"Koopmans liked it all. Whether it was China’s repeated delay of the mission, the lack of raw data, China’s veto over the composition of the team Marion Koopmans did not speak a single word of criticism"

janbhommel.nl/ask-marion-4-t…
12. Gain of Function

"I strongly suspect that this successful Gain-of-function investigation by Ron Fouchier is the reason why Marion Koopmans and he did not sign the letter to The Lancet"
13. Funding
" For Koopmans and Fouchier too, if it were proven that the SARS-CoV-2 virus had escaped from the WIV, it would have disastrous consequences getting funds for Gain-of-function experiments with influenza viruses, just as it would have for Ralph Baric"
14. Funding (2)

Under "Research Projects"

erasmusmc.nl/en/research/re…

Anyone got a Calculator, or a little Dutch Abacus?
15. Erasmus MC, EcoHealth Alliance,Koopmans

Thick as Thieves, Virus Thieves!



@uacjess
16. Unroll Marion Koopmans @threadreaderapp

and send that email to SAGO@who.int

with subject:

“Public comments on SAGO members” by 27 October 2021

• • •

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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Aug 13
Baric's hACE2 transgenic mice were shared with the WIV for GOF like virus experiments designed to "assess" the potential for coronaviruses to jump from animals to humans.

We asked how many were sent to WIV

researchgate.net/publication/35…

Record via @jhas5 here

arlo.riseforanimals.org/entity/univers… x.com/jhas5/status/1…
2. Baric spills his humanized mice beans to Father Racaniello during a heart rending TWIV confessional in March 2020, admitting he sent his hACE2 mice to WIV in 2018 (as part of the infamous NIAID grant mentioned above):



Timestamp; 01:00:30 - 01:03:15microbe.tv/twiv/twiv-591/
3. Daszak

"Our R01 work shows that bats in South China harbor many SARSr-CoVs (50+), some of which are able to use human ACE2 to enter human cells, infecting humanized mouse models to cause SARS-like illness, and evade available therapies or vaccines"

ecohealthalliance.org/wp-content/upl…
Read 9 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Aug 11
1. New England Biolabs & Novagen

The amplified Rp3-S1 fragment was digested with BamHI & XhoI & ligated into the expression vector pMAL-C2X (New England Biolabs).

The 5 truncated fragments were digested with BamHI & XhoI and ligated into the expression vector pET32a (Novagen).
2. Promega & Takara

Protein expression & purification

After purification, recombinant plasmid was transformed into BL21 competent cells (purchased from Promega)

Recovered PCR product & expression vector pET32a were digested with restriction endonucleases BamHI & XhoI (Takara).
3. Takara (2)

After DNA recovery, the PCR product was ligated to the expression vector pET32a using T4 DNA ligase (Takara)
Read 32 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 26
@breakfast_dogs @gadboit @NLink247 1. Insect cell baculovirus cell cultures become contaminated with insect genomic material.
2. Replication process can lead to presence of viral DNA & cellular components in culture medium.
3. This LEADS to contamination of viral product with baculovirus & insect cell-derived DNA.
@breakfast_dogs @gadboit @NLink247 Contamination

4. Expression of SC-2 Spike RBD Using the Insect Cell-Baculovirus System

pmc.ncbi.nlm.nih.gov/articles/PMC97…

5. Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture

pmc.ncbi.nlm.nih.gov/articles/PMC71…

6. insect cell lines
sciencedirect.com/science/articl…
@breakfast_dogs @gadboit @NLink247 Did WIV use insect cell systems with SARS-C0V-2?

7. Insect cell baculovirus expression systems

x.com/BillyBostickso…

8. DENG Fei, Chief Investigator in Research Group on Viruses Resources at WIV engineered baculovirus-insect cell expression systems.

buzzchronicles.com/b/all/17652/
Read 13 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 22
1. Kadlec awaiting confirmation for his new role

2. Kadlec's report references pangolins 76 times

bush.tamu.edu/wp-content/upl…

3. Time to read my pangolin coronavirus report?

researchgate.net/publication/39…

4. DRASTIC research cited
@gdemaneuf @ydeigin @BillyBostickson @C_Small_ x.com/BoDeepest/stat…
1. the article linked is quite good, more comments and analysis will be posted here soon

city-journal.org/article/robert…Image
2. Possible lab manipulations

"2) insertion of a pangolin virus recombinant spike or the spike’s RBD protein that includes a sequence for an FCS and an integrin-binding protein" Image
Read 76 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 9
Pressure is needed on people like @realannapaulina @RepLuna & @GOPoversight team as their mandate may dissolve in August.

They did Kennedy, but they are supposed to do UFO files & CoVid-19 origins before they dissolve.

We also need to know about the subpoenas sent by @RandPaul
What was promised and demanded (for the record)

oversight.house.gov/release/rep-lu…

oversight.house.gov/release/chairm…
If you are a concerned US citizen please directly contact the people listed here:

oversight.house.gov/subcommittee/t…
Read 8 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 1
🧵Rev. Billy's Lab Leak Cover-Up Sermon

1. Brethren, hear me now, for we stand at the precipice of a great & grievous iniquity!

The world has been ensnared by a monstrous conspiracy, a vile machination spun by a clique of scientists, experts, media scribes & state overlords!
🧵2. Let us never forget!

Those who, in their arrogance, have sought to veil the truth of the COVID pestilence!

And I say to you today, that this is no mere error, but a deliberate, diabolical plot to obscure the origins of a plague that has scourged the nations!
🧵3. The Vocabulary of Deception

Let us expose this falsehood, this house of deceit

& the duties of the vigilant in these perilous times!

Oh, what a wretched lexicon these deceivers employ! Their words are cloaked in the garb of science, yet they reek of falsehood & treachery!
Read 23 tweets

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