"Viral isolates will remain at the Wuhan Institute of Virology initially. Isolates, reagents and any other
products, will be made available to other NIH-funded researchers via applicable WIV and EHA Material Transfer Agreements and/or icensing agreements"
3. Cells (Page 203)
• Records are maintained for each of the cell lines regarding 1. the origin of the cell lines 2. when they were resuscitated 3. number of passages 4. all test results 5. any unique distinguishing growth behavior 6. any known genetic features.
4. Cell lInes (Page 203)
When receiving cell lines, lab members initially keep them isolated from other authenticated cell lines until mycoplasma testing & STR marker profiling is performed
All cell lines must be authenticated before commencing experimental work with them
5. Page 528
Recombinant and Wildtype Viruses and Mutant Derivatives.
All viruses will be validated and certified pure of contaminating viruses prior to use or shipment to other laboratories
6. Audit Trail (Page 527)
"UNC & WIV will implement an "audit trail" that tracks animals used in experimental investigations (Aim 3) from parents, through birth, shipment, experimentation, results, QC & analyses, providing outside researchers the ability to track experiments.."
7. Specific Aim 3 is on Page 512 and 272
Humanized mice for experimental infection for Specific Aim 3 (Page 512)
Specific Aim 3: Testing predictions of CoV inter-species transmission (Page 272)
8. RPPR reporting requirements
Research conducted at the following site(s) must be reported in your RPPR:
lnstitut Pasteur du Cambodge, CAMBODIA
King Chulalongkorn Memorial Hospital, THAILAND
Hanoi Agricultural University, VIETNAM
National Animal Health Laboratory, LAOS
9. Also:
San Pya Clinic, BURMA
Primate Research Center at Bogor Agricultural University, INDONESIA
Conservation Medicine, Ltd, MALAYSIA
"we would like to request prior approval for collecting non-human animal samples in 7 countries:
Cambodia, Indonesia, Lao PDR , Malaysia, Myanmar, Thailand, and Vietnam" (Page 100)
"During shooting in Thailand, I was shocked by Daszak’s fast & loose with facts, his refusal to acknowledge his conflict of interest,& his denial of his gain-of-function res in collaboration with Wuhan lab"
You guessed right, i hope?
@janeqiuchina
She continued:
"I was equally shocked by Daszak’s constant self-promotion and how effective it was."
@janeqiuchina About the film:
"Blame: Bats, Politics & a Planet Out of Balance"
"Christian Frei seemed entranced by hero worshipping, apparently having lost all sense of objectivity & critical judgment. I felt strongly then, as I do now, that the film was a blatant piece of propaganda"
Bloody Hell, it's worse than even I imagined in my darkest nightmares about #scamdemia.
Time to spend some of this tax money on local electronics, biological, ecological, IT, optical, chemistry, engineering & physics labs run by local amateurs for the benefit of our communities?
Short 🧵on Primer & PCR Test issues (Pangolin Covs)
1. The key issue with pangolins and Pcovs is that:
It was discovered that the standard PCR tests used in 2020, and often still used, failed to actually detect many of the betacoronaviruses generously hosted by the pangolins.
2. Unless
The samples are retested with bespoke primers & more accurate PCR tests available now,
We will not know exactly whIch Pangolin (or bat) coronaviruses were hosted by Pangolins at WIV & elsewhere pre-pandemic.
However, "they" (I will name who they are later) should, and perhaps in the future, may retest the stored samples at WIV, IPB, SCAU AND GIABR, to clarify the question of which coronaviruses were present in their pangolin samples.
2. May have been for research and commercial purposes, but they were caught bang to rights.
"LIU initially stated that he did not know what the materials were and that someone must have put them into his bag"
3. When asked why someone would put them into his bag, LIU stated that he did not know, and that maybe he had accidentally put the materials there. After further questioning, LIU acknowledged that the materials were different strains of the pathogen Fusarium graminearum