"Viral isolates will remain at the Wuhan Institute of Virology initially. Isolates, reagents and any other
products, will be made available to other NIH-funded researchers via applicable WIV and EHA Material Transfer Agreements and/or icensing agreements"
3. Cells (Page 203)
• Records are maintained for each of the cell lines regarding 1. the origin of the cell lines 2. when they were resuscitated 3. number of passages 4. all test results 5. any unique distinguishing growth behavior 6. any known genetic features.
4. Cell lInes (Page 203)
When receiving cell lines, lab members initially keep them isolated from other authenticated cell lines until mycoplasma testing & STR marker profiling is performed
All cell lines must be authenticated before commencing experimental work with them
5. Page 528
Recombinant and Wildtype Viruses and Mutant Derivatives.
All viruses will be validated and certified pure of contaminating viruses prior to use or shipment to other laboratories
6. Audit Trail (Page 527)
"UNC & WIV will implement an "audit trail" that tracks animals used in experimental investigations (Aim 3) from parents, through birth, shipment, experimentation, results, QC & analyses, providing outside researchers the ability to track experiments.."
7. Specific Aim 3 is on Page 512 and 272
Humanized mice for experimental infection for Specific Aim 3 (Page 512)
Specific Aim 3: Testing predictions of CoV inter-species transmission (Page 272)
8. RPPR reporting requirements
Research conducted at the following site(s) must be reported in your RPPR:
lnstitut Pasteur du Cambodge, CAMBODIA
King Chulalongkorn Memorial Hospital, THAILAND
Hanoi Agricultural University, VIETNAM
National Animal Health Laboratory, LAOS
9. Also:
San Pya Clinic, BURMA
Primate Research Center at Bogor Agricultural University, INDONESIA
Conservation Medicine, Ltd, MALAYSIA
"we would like to request prior approval for collecting non-human animal samples in 7 countries:
Cambodia, Indonesia, Lao PDR , Malaysia, Myanmar, Thailand, and Vietnam" (Page 100)
Turning and turning in the petri dish,
The scientists cannot hear the warnings;
Genes recombine; the barriers cannot hold,
Evil virology is loosed upon the world,
The blood-dimmed tide is loosed, and everywhere
The ceremony of innocence is drowned;
The best lack critical thinking, while the professors
Are full of furious bias.
Surely some revelation is at hand;
Surely the cover up is now banned?
Lab Leak! Hardly are those words out
When a vast image out of Human Folly
Troubles my sight:
3. Somewhere in Wuhan
somewhere in the cell lines of a Chinese laboratory
A shape with pangolin body and the head of a bat,
A gaze blank and pitiless as the sun,
Is moving its long tongue, while all about it
Reel shadows of the indignant molested bats.
🧵As promised, here is my counter critique & rebuttal of some points made by @gadboit about the Pangolin Papers Hypothesis.
His theory seems to be that SARS-COV-2 emerged like Aphrodite, from BANAL BAT viruses, possibly with some FCS tweaking & no Pangolins coronaviruses needed.
@gadboit 🧵2. @gadboit claims BANAL-52’s 96.8% similarity & ACE2 binding make it closer to SARS-CoV-2 than PCoVs (~91%) & that PCoV features could come from bat CoVs.
But GD PCoV’s RBD (97% identity) needs no mutations for hACE2, unlike BANALs.
A. “The Pangolin Papers” presents a robust argument for the lab-mediated origin of SARS-CoV-2, particularly centered on experiments conducted at the Wuhan Institute of Virology (WIV) during 2018 and 2019.
1. A Modest Proposal for the Fair Imprisonment of Women and the Liberation of Men
By a Concerned Citizen of Reason,
Published in The Rational Gazette on X
March 24, 2025
2. Dire Injustice!
It is a lamentable truth, oft repeated in the progressive broadsheets and the sanctimonious halls of modern discourse, that the state of our prisons reveals a dire injustice.
3. Gaol'd Wretches
We are told, with furrowed brows and trembling voices, that Black men, comprising a mere 4% of the good people of England and Wales, yet numbering 13% of our gaol’d wretches, suffer under the cruel yoke of systemic discrimination.
@Ticklicker56 @angie_rasmussen @Dissenting2020 @DrStrangeLovett @Rebecca21951651 @All_New_to_This @VaughnMises @R_H_Ebright @Florin_Uncovers @ban_epp_gofroc @MJnanostretch @Muller_Lab @SolidEvidence @ydeigin @ciukzwil @tgof137 @StavaRune @VBruttel @FondueMean @tom335363 @BioSRP @stevenemassey @jbloom_lab @BallouxFrancois @Ayjchan @mattwridley RML expertise in biodefense inlcudes tick & flea agents, so I doubt she would want to discuss how many ticks they have or what they inject them with.