Marine Laporte Profile picture
Jan 13, 2022 9 tweets 5 min read Read on X
“Cryo-ExM” or “how to see (almost) everything you ever dreamt of”
Want to know more ?!? Check our story out with @CentrioleLab @nikolai_klena in @naturemethods: nature.com/articles/s4159… which combines cryo-fixation/Freeze substitution with #expansion #microscopy (#UExM).
1/Cryo-fixation, as demonstrated in electron microscopy, outperformed chemical fixations on subcellular structure such as the endoplasmic reticulum
2/Cryo-fixation circumvent the artifact of chemical fixations such as loss of antigenicity as seen with the mitochondrial outer membrane marker TOMM20
3/Cryo-ExM allows to catch “dynamic events” such as interactions between endoplasmic reticulum and microtubules or mitochondria
4/Cryo-fixation preserves microtubule organization of mitotic and ciliated cells
5/…Actin cytoskeleton
6/Or even the “liquid-like droplet” pyrenoid structure from Chlamydomonas cells from @nikolai_klena...
7/As shown in Cryo-EM by @bengeliscious (Freeman et al., cell 2017)
8/ And so many more....
nature.com/articles/s4159…

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