Furin cleavage site data and discussion included. Identical sequence stretch on S. cerevisiae chromosome XIII identified and suggested as potential recombination donor for the furin site insert in the SARS-CoV-2 spike glycoprotein
3. Revised version 3 includes new data regarding the origin of the unusual FCS in SARS-CoV-2. Indeed, with these new data & evidence, it suggests that this critical site is the product of a recombination event after synthetic passage in genetically manipulated S. cerevisiae cells
4. . Overall, the research presented in this article now provides strong evidence that SARS-CoV-2 has an origin in synthetic yeast, and as such is not a product of natural evolution alone.
5. Highlighted Revisions - 1
"At this junction, we detect a highly specific stretch of yeast DNA encoding for the critical furin cleavage site insert PRRA, which has not been seen in other lineage b betacoronaviruses"
6. Highlighted Revisions - 2
"Our data specifically allow the identification of
the yeast S. cerevisiae as a potential recombination donor for the critical furin cleavage site in SARS-CoV-2"
7. Highlighted Revisions - 3
"Of special interest in this analysis was a 16 base sequence TTCTCCTCGGCGGGCA near P2 between position 23599 & 23614, which corresponded to the furin cleavage site & identically aligned with bases [810386..810401] from S. cerevisiae chromosome XIII"
8. Highlighted Revisions - 4
"Yeast (S. cerevisiae) standardized BLAT z-scores representing the relative homology signal from all alignment scores in 13 representative SARS-related coronaviruses"
Note that only, SARS-COV 1, SARS -COV 2 and RaTG13 show Yeast anomalies.
9. Highlighted Revisions - 5
"According to our data, this cleavage site is specifically compatible with a recombination event including chromosome XIII of S. cerevisiae, which shares a unique nucleotide sequence that encodes
the necessary insert PRRA"
10. Any questions?
Corresponding author: Andreas Martin Lisewski
a.lisewski@jacobs-university.de
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1. Heinz Felfe was head of the Soviet counter-intelligence Department & ran a long running project called "Panoptikum" to uncover a high level "mole" within the West German Intelligence Service...
In the end, the target of "Panoptikum" would turn out to be Heinz Felfe himself!
2. His Memoirs (in German)
Heinz Felfe published his memoir in 1986 under the title "In the Service of the enemy: Ten years as Moscow's man in the Federal Intelligence Service"
"During initial stages of a forensic investigation of Wuhan Labs, all 4 evaluation tools including Grunow & Finke's epidemiological assessment criteria would be useful adjuncts to the investigation framework of Pilch et al."
"The Radosavljevic–Belojevic method for outbreak scoring & differentiation shows that official assumptions of its natural origin is questionable & highlights the probability that the pathogen was "accidentally" introduced to humans"
@tgof137 @Rebecca21951651 @_everythingism @AtomsksSanakan @CallumJCParr @jhas5 1. It is best to do your own reading & consult the research of others, such as #DRASTIC, who published all the most accurate details on labs, locations, engineering flaws, viral databases, field sampling of bats, lack of proper biosafety, etc. years ago: