Furin cleavage site data and discussion included. Identical sequence stretch on S. cerevisiae chromosome XIII identified and suggested as potential recombination donor for the furin site insert in the SARS-CoV-2 spike glycoprotein
3. Revised version 3 includes new data regarding the origin of the unusual FCS in SARS-CoV-2. Indeed, with these new data & evidence, it suggests that this critical site is the product of a recombination event after synthetic passage in genetically manipulated S. cerevisiae cells
4. . Overall, the research presented in this article now provides strong evidence that SARS-CoV-2 has an origin in synthetic yeast, and as such is not a product of natural evolution alone.
5. Highlighted Revisions - 1
"At this junction, we detect a highly specific stretch of yeast DNA encoding for the critical furin cleavage site insert PRRA, which has not been seen in other lineage b betacoronaviruses"
6. Highlighted Revisions - 2
"Our data specifically allow the identification of
the yeast S. cerevisiae as a potential recombination donor for the critical furin cleavage site in SARS-CoV-2"
7. Highlighted Revisions - 3
"Of special interest in this analysis was a 16 base sequence TTCTCCTCGGCGGGCA near P2 between position 23599 & 23614, which corresponded to the furin cleavage site & identically aligned with bases [810386..810401] from S. cerevisiae chromosome XIII"
8. Highlighted Revisions - 4
"Yeast (S. cerevisiae) standardized BLAT z-scores representing the relative homology signal from all alignment scores in 13 representative SARS-related coronaviruses"
Note that only, SARS-COV 1, SARS -COV 2 and RaTG13 show Yeast anomalies.
9. Highlighted Revisions - 5
"According to our data, this cleavage site is specifically compatible with a recombination event including chromosome XIII of S. cerevisiae, which shares a unique nucleotide sequence that encodes
the necessary insert PRRA"
10. Any questions?
Corresponding author: Andreas Martin Lisewski
a.lisewski@jacobs-university.de
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@breakfast_dogs @gadboit @NLink247 1. Insect cell baculovirus cell cultures become contaminated with insect genomic material. 2. Replication process can lead to presence of viral DNA & cellular components in culture medium. 3. This LEADS to contamination of viral product with baculovirus & insect cell-derived DNA.
@breakfast_dogs @gadboit @NLink247 Contamination
4. Expression of SC-2 Spike RBD Using the Insect Cell-Baculovirus System
1. Brethren, hear me now, for we stand at the precipice of a great & grievous iniquity!
The world has been ensnared by a monstrous conspiracy, a vile machination spun by a clique of scientists, experts, media scribes & state overlords!
🧵2. Let us never forget!
Those who, in their arrogance, have sought to veil the truth of the COVID pestilence!
And I say to you today, that this is no mere error, but a deliberate, diabolical plot to obscure the origins of a plague that has scourged the nations!
🧵3. The Vocabulary of Deception
Let us expose this falsehood, this house of deceit
& the duties of the vigilant in these perilous times!
Oh, what a wretched lexicon these deceivers employ! Their words are cloaked in the garb of science, yet they reek of falsehood & treachery!
"During shooting in Thailand, I was shocked by Daszak’s fast & loose with facts, his refusal to acknowledge his conflict of interest,& his denial of his gain-of-function res in collaboration with Wuhan lab"
You guessed right, i hope?
@janeqiuchina
She continued:
"I was equally shocked by Daszak’s constant self-promotion and how effective it was."
@janeqiuchina About the film:
"Blame: Bats, Politics & a Planet Out of Balance"
"Christian Frei seemed entranced by hero worshipping, apparently having lost all sense of objectivity & critical judgment. I felt strongly then, as I do now, that the film was a blatant piece of propaganda"