Furin cleavage site data and discussion included. Identical sequence stretch on S. cerevisiae chromosome XIII identified and suggested as potential recombination donor for the furin site insert in the SARS-CoV-2 spike glycoprotein
3. Revised version 3 includes new data regarding the origin of the unusual FCS in SARS-CoV-2. Indeed, with these new data & evidence, it suggests that this critical site is the product of a recombination event after synthetic passage in genetically manipulated S. cerevisiae cells
4. . Overall, the research presented in this article now provides strong evidence that SARS-CoV-2 has an origin in synthetic yeast, and as such is not a product of natural evolution alone.
5. Highlighted Revisions - 1
"At this junction, we detect a highly specific stretch of yeast DNA encoding for the critical furin cleavage site insert PRRA, which has not been seen in other lineage b betacoronaviruses"
6. Highlighted Revisions - 2
"Our data specifically allow the identification of
the yeast S. cerevisiae as a potential recombination donor for the critical furin cleavage site in SARS-CoV-2"
7. Highlighted Revisions - 3
"Of special interest in this analysis was a 16 base sequence TTCTCCTCGGCGGGCA near P2 between position 23599 & 23614, which corresponded to the furin cleavage site & identically aligned with bases [810386..810401] from S. cerevisiae chromosome XIII"
8. Highlighted Revisions - 4
"Yeast (S. cerevisiae) standardized BLAT z-scores representing the relative homology signal from all alignment scores in 13 representative SARS-related coronaviruses"
Note that only, SARS-COV 1, SARS -COV 2 and RaTG13 show Yeast anomalies.
9. Highlighted Revisions - 5
"According to our data, this cleavage site is specifically compatible with a recombination event including chromosome XIII of S. cerevisiae, which shares a unique nucleotide sequence that encodes
the necessary insert PRRA"
10. Any questions?
Corresponding author: Andreas Martin Lisewski
a.lisewski@jacobs-university.de
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"During shooting in Thailand, I was shocked by Daszak’s fast & loose with facts, his refusal to acknowledge his conflict of interest,& his denial of his gain-of-function res in collaboration with Wuhan lab"
You guessed right, i hope?
@janeqiuchina
She continued:
"I was equally shocked by Daszak’s constant self-promotion and how effective it was."
@janeqiuchina About the film:
"Blame: Bats, Politics & a Planet Out of Balance"
"Christian Frei seemed entranced by hero worshipping, apparently having lost all sense of objectivity & critical judgment. I felt strongly then, as I do now, that the film was a blatant piece of propaganda"
Bloody Hell, it's worse than even I imagined in my darkest nightmares about #scamdemia.
Time to spend some of this tax money on local electronics, biological, ecological, IT, optical, chemistry, engineering & physics labs run by local amateurs for the benefit of our communities?
Short 🧵on Primer & PCR Test issues (Pangolin Covs)
1. The key issue with pangolins and Pcovs is that:
It was discovered that the standard PCR tests used in 2020, and often still used, failed to actually detect many of the betacoronaviruses generously hosted by the pangolins.
2. Unless
The samples are retested with bespoke primers & more accurate PCR tests available now,
We will not know exactly whIch Pangolin (or bat) coronaviruses were hosted by Pangolins at WIV & elsewhere pre-pandemic.
However, "they" (I will name who they are later) should, and perhaps in the future, may retest the stored samples at WIV, IPB, SCAU AND GIABR, to clarify the question of which coronaviruses were present in their pangolin samples.
2. May have been for research and commercial purposes, but they were caught bang to rights.
"LIU initially stated that he did not know what the materials were and that someone must have put them into his bag"
3. When asked why someone would put them into his bag, LIU stated that he did not know, and that maybe he had accidentally put the materials there. After further questioning, LIU acknowledged that the materials were different strains of the pathogen Fusarium graminearum