Furin cleavage site data and discussion included. Identical sequence stretch on S. cerevisiae chromosome XIII identified and suggested as potential recombination donor for the furin site insert in the SARS-CoV-2 spike glycoprotein
3. Revised version 3 includes new data regarding the origin of the unusual FCS in SARS-CoV-2. Indeed, with these new data & evidence, it suggests that this critical site is the product of a recombination event after synthetic passage in genetically manipulated S. cerevisiae cells
4. . Overall, the research presented in this article now provides strong evidence that SARS-CoV-2 has an origin in synthetic yeast, and as such is not a product of natural evolution alone.
5. Highlighted Revisions - 1
"At this junction, we detect a highly specific stretch of yeast DNA encoding for the critical furin cleavage site insert PRRA, which has not been seen in other lineage b betacoronaviruses"
6. Highlighted Revisions - 2
"Our data specifically allow the identification of
the yeast S. cerevisiae as a potential recombination donor for the critical furin cleavage site in SARS-CoV-2"
7. Highlighted Revisions - 3
"Of special interest in this analysis was a 16 base sequence TTCTCCTCGGCGGGCA near P2 between position 23599 & 23614, which corresponded to the furin cleavage site & identically aligned with bases [810386..810401] from S. cerevisiae chromosome XIII"
8. Highlighted Revisions - 4
"Yeast (S. cerevisiae) standardized BLAT z-scores representing the relative homology signal from all alignment scores in 13 representative SARS-related coronaviruses"
Note that only, SARS-COV 1, SARS -COV 2 and RaTG13 show Yeast anomalies.
9. Highlighted Revisions - 5
"According to our data, this cleavage site is specifically compatible with a recombination event including chromosome XIII of S. cerevisiae, which shares a unique nucleotide sequence that encodes
the necessary insert PRRA"
10. Any questions?
Corresponding author: Andreas Martin Lisewski
a.lisewski@jacobs-university.de
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Turning and turning in the petri dish,
The scientists cannot hear the warnings;
Genes recombine; the barriers cannot hold,
Evil virology is loosed upon the world,
The blood-dimmed tide is loosed, and everywhere
The ceremony of innocence is drowned;
The best lack critical thinking, while the professors
Are full of furious bias.
Surely some revelation is at hand;
Surely the cover up is now banned?
Lab Leak! Hardly are those words out
When a vast image out of Human Folly
Troubles my sight:
3. Somewhere in Wuhan
somewhere in the cell lines of a Chinese laboratory
A shape with pangolin body and the head of a bat,
A gaze blank and pitiless as the sun,
Is moving its long tongue, while all about it
Reel shadows of the indignant molested bats.
🧵As promised, here is my counter critique & rebuttal of some points made by @gadboit about the Pangolin Papers Hypothesis.
His theory seems to be that SARS-COV-2 emerged like Aphrodite, from BANAL BAT viruses, possibly with some FCS tweaking & no Pangolins coronaviruses needed.
@gadboit 🧵2. @gadboit claims BANAL-52’s 96.8% similarity & ACE2 binding make it closer to SARS-CoV-2 than PCoVs (~91%) & that PCoV features could come from bat CoVs.
But GD PCoV’s RBD (97% identity) needs no mutations for hACE2, unlike BANALs.
A. “The Pangolin Papers” presents a robust argument for the lab-mediated origin of SARS-CoV-2, particularly centered on experiments conducted at the Wuhan Institute of Virology (WIV) during 2018 and 2019.
1. A Modest Proposal for the Fair Imprisonment of Women and the Liberation of Men
By a Concerned Citizen of Reason,
Published in The Rational Gazette on X
March 24, 2025
2. Dire Injustice!
It is a lamentable truth, oft repeated in the progressive broadsheets and the sanctimonious halls of modern discourse, that the state of our prisons reveals a dire injustice.
3. Gaol'd Wretches
We are told, with furrowed brows and trembling voices, that Black men, comprising a mere 4% of the good people of England and Wales, yet numbering 13% of our gaol’d wretches, suffer under the cruel yoke of systemic discrimination.
@Ticklicker56 @angie_rasmussen @Dissenting2020 @DrStrangeLovett @Rebecca21951651 @All_New_to_This @VaughnMises @R_H_Ebright @Florin_Uncovers @ban_epp_gofroc @MJnanostretch @Muller_Lab @SolidEvidence @ydeigin @ciukzwil @tgof137 @StavaRune @VBruttel @FondueMean @tom335363 @BioSRP @stevenemassey @jbloom_lab @BallouxFrancois @Ayjchan @mattwridley RML expertise in biodefense inlcudes tick & flea agents, so I doubt she would want to discuss how many ticks they have or what they inject them with.