In a recent lung cancer pilot study, we identified and quantified a record number of 13,187 protein groups with our next-generation single-shot proteomics technology HRM.
This was achieved using FAIMS-DIA with highest resolution chromatography and FAIMS Exploris 480. (1/n)
This was achieved using FAIMS-DIA with highest resolution chromatography and FAIMS Exploris 480. (1/n)
The depth of analysis was superior to the one achieved in the Deep Human Proteome Atlas (deep fractionated tissue with multiple MS acquisitions, Wang et al., 2019, Mol Syst Biol).
Comprehensively, we could identify 414 oncogenes, 392 transcription factors, 837 plasma membrane proteins (25 GPCR), and 316 kinases covering a dynamic range of 10. In addition, 0.25 Mio peptides (including modifications) were measured in totality.
The cancer types could be separated by PCA and allowed comprehensive picture of the altered pathways.
This represents the deepest single shot proteome profiling reported to date to the best of our knowledge.
(4/4)
This represents the deepest single shot proteome profiling reported to date to the best of our knowledge.
(4/4)
The data set of the 24 clinical samples (healthy and cancer) had 13% missing values on protein level for the 13,187 proteins or 87% completeness. :) #CancerMoonshot #cancerresearch #proteomics #clinicalresearch
• • •
Missing some Tweet in this thread? You can try to
force a refresh
