Thread by @kirtiprakash25

#SIMposium starts today at 8:45 am (BST), abt 30 mins from now!

Full program here👇
royalsociety.org/science-events…

See you soon!

Follow the conference with #SIMposium #SIMposium2022

cc @RSocPublishing @HeintzmannLab @LSchermelleh @beniroquai @StefanieReiche6

@HeintzmannLab starts the #SIMposium with thoughts and a wishlist for resolution criteria!

He will be giving a historical overview of super-resolution microscopy during the panel discussion session at 12:00 (BST).

Priceless discussion!

Abbe resolution demystified!

To all contemporary scientists: what equation or finding would you like to be known for or have on your gravestone?😉

#SIMposium

If you missed the @HeintzmannLab talk, you can read more here:
royalsocietypublishing.org/doi/pdf/10.109…
royalsocietypublishing.org/doi/pdf/10.109…

#SIMposium

@JamesDManton starts boldly with "SIM: a counterfactual history"😉 🔥

Read more here:
royalsocietypublishing.org/doi/pdf/10.109…

@LSchermelleh starting his talk reminding us about our initial plans for #SIMposium that got hit by #COVID19

Here's a brief history of superresolution microscopy!

Read more here:
royalsocietypublishing.org/doi/pdf/10.109…

#SIMposium

@LSchermelleh @HeintzmannLab @beniroquai @RSocPublishing

@LSchermelleh these are awesome images of the cell nucleus!

SIMcheck -> a very imp tool for quality control!

#SIMpoisum

Colin Sheppard talking now on "Structured illumination microscopy and image scanning microscopy: A comparison"
People interested in theory should not miss this talk!

Read more here:
royalsocietypublishing.org/doi/pdf/10.109…

Is Colin Sheppard on Twitter?

#SIMposium

In 1982, a computer cost Colin Sheppard $20,000 when his house was about $9,000!

Priceless discussion!

#SIMposium

After 22 years, @JoergEnderlein proves the principle of Image Scanning Microscopy!

And not many citations for Colin Sheppard's 1988 paper on "Super-resolution in Confocal Imaging" during this period!

"I have been using Bessel beams since 1977 when most people won't have even heard of it" - Colin Sheppard

#SIMposium

A lot of disagreements at #SIMposium!
Things warming up for the panel discussion at 12:00, don't miss it!

Great summary slide by Colin Sheppard!

SIM and ISM are basically inverses of each other😉

@AndrewGYork, any thoughts?

#SIMposium

@Orangeroad2017 now talking on "Overcoming physical resolution limits of fluorescence microscopes with sparse deconvolution"

The reference paper:
royalsocietypublishing.org/doi/pdf/10.109…

#SIMposium

"Sparsity is another general feature (prior knowledge) of fluorescence microscopy" - @Orangeroad2017

#SIMposium

SIM milestones by @HeintzmannLab at the #SIMposium panel discussion!

What a great first session at #SIMposium!

Learnt so much! Thanks to all the speakers and panel discussion leaders 👇
@HeintzmannLab @JamesDManton @LSchermelleh @Orangeroad2017 Colin Sheppard @lidong1208 @RetoPaul @huserlab @mueller_physics Christoph Cremer @FourierPower

The second session started with @RetoPaul talking on "Light-sheet microscopy with multi-directional structured illumination"

Now @huserlab talking on "High-speed and cost-efficient super-resolution structured illumination microscopy enabled entirely by fibre optics"

#SIMposium

@lidong1208 now talking on "Multi-SIM via deep learning algorithm for super-resolution live imaging"

#SIMposium

@FourierPower now talking on "Home-built SIM with SLM and Multifocus Microscopy"

Also, our first female speaker of the day! But please be assured that there will be many more tomorrow!

#SIMposium

@mueller_physics talks on "DMD for cost-effective and fast multi-colour SIM"
More here:
royalsocietypublishing.org/doi/pdf/10.109…

Next talk by Hai Gong on "GPU-accelerated real-time SIM reconstruction with hexagonal patterns"
royalsocietypublishing.org/doi/pdf/10.109…

#SIMposium

The last talk by Mike Shaw and @microRussell on "mmSIM: an open toolbox for accessible structured illumination microscopy"

Paper here:
royalsocietypublishing.org/doi/pdf/10.109…

This will be followed by a panel discussion on "Biological application of SIM" @LSchermelleh

#SIMposium

Very interesting last panel discussion!
Thanks, @JLS_Lab @FourierPower Mike Shaw @huserlab @lidong1208 @Orangeroad2017 @LSchermelleh Lisa Cameron!

We continue the discussion on Open Microscopy tomorrow with @HohlbeinLab @haesleinhuepf @beniroquai @StrambioLab

Day 2 at the #SIMposium starts at 9 am (BST) with a keynote talk from @S_LevequeFort on "Modulated excitation for enhanced single-molecule localisation microscopy"
royalsocietypublishing.org/doi/10.1098/rs…

See you all soon!

@S_LevequeFort reveals the papers that motivated her to come up with ModLoc!

#SIMposium

Christoph Cremer now talks on "Spatially modulated illumination microscopy: application perspectives in
nuclear nanostructure analysis"

#SIMposium

Next talk by @kirtiprakash25 on "At the molecular resolution with MINFLUX?"

Paper here:
royalsocietypublishing.org/doi/full/10.10…

#SIMposium

The next talks by Miguel Boland on "Upscaling SIM reconstruction via Deep Learning"
royalsocietypublishing.org/doi/full/10.10…

Followed by Kai Wicker on picoSIM
royalsocietypublishing.org/doi/full/10.10…

#SIMposium

@beniroquai now talking on "Modular Microscopy – not just a Toy"

Paper here:
royalsocietypublishing.org/doi/full/10.10…

Bene follows this talk with a workshop on @OpenUc2 - an open-source system for optics (The power in your
pocket)
github.com/openUC2/UC2-GIT

Awesome stuff!!!

#SIMposium

@StefanieReiche6 chairs the last #SIMposium session!
Starts by highlighting some of her creative work using structured patterns!

@kvijayraghavan @PrinSciAdvGoI - is that you in the image below?

#SIMposium

The last session starts with a keynote talk from @JLS_Lab on "Looking under the hood of cells: from single-molecule dynamics to whole-cell organelle reconstructions"

Some mind-blowing images, reconstructions coming up this session!

#SIMposium

Now Florian Schock talks on "Structured illumination ophthalmoscope: super-resolution microscopy
on the living human eye"

Paper here:
royalsocietypublishing.org/doi/full/10.10…

Nice use of SIM for medical applications!

#SIMposium

The next talks in the session by Kseniya Korobchevskaya on "Extended mechanical force measurements using SIM"
royalsocietypublishing.org/doi/full/10.10…

And by David McFadden on "Quality control of image sensors using Gaseous Tritium Light Sources"
royalsocietypublishing.org/doi/full/10.10…

#SIMposium

After the coffee break, @kirtiprakash25 talks on "Laser-free super-resolution microscopy (LFSM)"

royalsocietypublishing.org/doi/full/10.10……

@ReynaudEmmanuel do you like the LFSM acronym, to go with the light-sheet microscopy acronyms?😉

#SIMposium

@HohlbeinLab now talks on "Enabling single-molecule localisation microscopy in turbid food
emulsions"

Paper here:
royalsocietypublishing.org/doi/full/10.10…

Happy to learn that #miCube is spreading and has several new add-ons!

#SIMposium

The last two talks at the #SIMposium by @StrambioLab on "Open microscopy documentation for the real world"

And by @haesleinhuepf on "Clesperanto: Open-source GPU-accelerated image processing"

@haesleinhuepf seems to have something new every time I hear him!

#SIMposium

We conclude the #SIMposium with a panel discussion on "Open Microscopy: Quo Vadis?"

Please join @HohlbeinLab @beniroquai @StrambioLab @haesleinhuepf @StefanieReiche6 to discuss the questions below!

We have been receiving several messages like the ones below👇

Glad that people enjoyed the #SIMposium meeting and thanks for joining us!

@LSchermelleh @HeintzmannLab @beniroquai @StefanieReiche6 @RSocPublishing

Needless to mention, we strategically planned the 2-day #SIMposium to end today on #Twosday 😊
@royalsociety, @beniroquai @LSchermelleh - remember?😉

We had a lot of great talks, which might be of historical significance!

#ADay2Remember