#SIMposium starts today at 8:45 am (BST), abt 30 mins from now!

Full program here👇

See you soon!

Follow the conference with #SIMposium #SIMposium2022

cc @RSocPublishing @HeintzmannLab @LSchermelleh @beniroquai @StefanieReiche6
@HeintzmannLab starts the #SIMposium with thoughts and a wishlist for resolution criteria!

He will be giving a historical overview of super-resolution microscopy during the panel discussion session at 12:00 (BST).

Priceless discussion!
Abbe resolution demystified!

To all contemporary scientists: what equation or finding would you like to be known for or have on your gravestone?😉

@JamesDManton starts boldly with "SIM: a counterfactual history"😉 🔥

Read more here:
@LSchermelleh starting his talk reminding us about our initial plans for #SIMposium that got hit by #COVID19
Here's a brief history of superresolution microscopy!

Read more here:


@LSchermelleh @HeintzmannLab @beniroquai @RSocPublishing
@LSchermelleh these are awesome images of the cell nucleus!

SIMcheck -> a very imp tool for quality control!

Colin Sheppard talking now on "Structured illumination microscopy and image scanning microscopy: A comparison"
People interested in theory should not miss this talk!

Read more here:

Is Colin Sheppard on Twitter?

In 1982, a computer cost Colin Sheppard $20,000 when his house was about $9,000!

Priceless discussion!

After 22 years, @JoergEnderlein proves the principle of Image Scanning Microscopy!

And not many citations for Colin Sheppard's 1988 paper on "Super-resolution in Confocal Imaging" during this period!
"I have been using Bessel beams since 1977 when most people won't have even heard of it" - Colin Sheppard

A lot of disagreements at #SIMposium!
Things warming up for the panel discussion at 12:00, don't miss it!
Great summary slide by Colin Sheppard!

SIM and ISM are basically inverses of each other😉

@AndrewGYork, any thoughts?

@Orangeroad2017 now talking on "Overcoming physical resolution limits of fluorescence microscopes with sparse deconvolution"

The reference paper:

"Sparsity is another general feature (prior knowledge) of fluorescence microscopy" - @Orangeroad2017

SIM milestones by @HeintzmannLab at the #SIMposium panel discussion!
What a great first session at #SIMposium!

Learnt so much! Thanks to all the speakers and panel discussion leaders 👇
@HeintzmannLab @JamesDManton @LSchermelleh @Orangeroad2017 Colin Sheppard @lidong1208 @RetoPaul @huserlab @mueller_physics Christoph Cremer @FourierPower
The second session started with @RetoPaul talking on "Light-sheet microscopy with multi-directional structured illumination"

Now @huserlab talking on "High-speed and cost-efficient super-resolution structured illumination microscopy enabled entirely by fibre optics"

@lidong1208 now talking on "Multi-SIM via deep learning algorithm for super-resolution live imaging"

@FourierPower now talking on "Home-built SIM with SLM and Multifocus Microscopy"

Also, our first female speaker of the day! But please be assured that there will be many more tomorrow!

@mueller_physics talks on "DMD for cost-effective and fast multi-colour SIM"
More here:

Next talk by Hai Gong on "GPU-accelerated real-time SIM reconstruction with hexagonal patterns"

The last talk by Mike Shaw and @microRussell on "mmSIM: an open toolbox for accessible structured illumination microscopy"

Paper here:

This will be followed by a panel discussion on "Biological application of SIM" @LSchermelleh

Very interesting last panel discussion!
Thanks, @JLS_Lab @FourierPower Mike Shaw @huserlab @lidong1208 @Orangeroad2017 @LSchermelleh Lisa Cameron!

We continue the discussion on Open Microscopy tomorrow with @HohlbeinLab @haesleinhuepf @beniroquai @StrambioLab
Day 2 at the #SIMposium starts at 9 am (BST) with a keynote talk from @S_LevequeFort on "Modulated excitation for enhanced single-molecule localisation microscopy"

See you all soon!
@S_LevequeFort reveals the papers that motivated her to come up with ModLoc!

Christoph Cremer now talks on "Spatially modulated illumination microscopy: application perspectives in
nuclear nanostructure analysis"

Next talk by @kirtiprakash25 on "At the molecular resolution with MINFLUX?"

Paper here:

The next talks by Miguel Boland on "Upscaling SIM reconstruction via Deep Learning"

Followed by Kai Wicker on picoSIM

@beniroquai now talking on "Modular Microscopy – not just a Toy"

Paper here:

Bene follows this talk with a workshop on @OpenUc2 - an open-source system for optics (The power in your

Awesome stuff!!!

@StefanieReiche6 chairs the last #SIMposium session!
Starts by highlighting some of her creative work using structured patterns!

@kvijayraghavan @PrinSciAdvGoI - is that you in the image below?

The last session starts with a keynote talk from @JLS_Lab on "Looking under the hood of cells: from single-molecule dynamics to whole-cell organelle reconstructions"

Some mind-blowing images, reconstructions coming up this session!

Now Florian Schock talks on "Structured illumination ophthalmoscope: super-resolution microscopy
on the living human eye"

Paper here:

Nice use of SIM for medical applications!

The next talks in the session by Kseniya Korobchevskaya on "Extended mechanical force measurements using SIM"

And by David McFadden on "Quality control of image sensors using Gaseous Tritium Light Sources"

After the coffee break, @kirtiprakash25 talks on "Laser-free super-resolution microscopy (LFSM)"


@ReynaudEmmanuel do you like the LFSM acronym, to go with the light-sheet microscopy acronyms?😉

@HohlbeinLab now talks on "Enabling single-molecule localisation microscopy in turbid food

Paper here:

Happy to learn that #miCube is spreading and has several new add-ons!

The last two talks at the #SIMposium by @StrambioLab on "Open microscopy documentation for the real world"

And by @haesleinhuepf on "Clesperanto: Open-source GPU-accelerated image processing"

@haesleinhuepf seems to have something new every time I hear him!

We conclude the #SIMposium with a panel discussion on "Open Microscopy: Quo Vadis?"

Please join @HohlbeinLab @beniroquai @StrambioLab @haesleinhuepf @StefanieReiche6 to discuss the questions below!
We have been receiving several messages like the ones below👇

Glad that people enjoyed the #SIMposium meeting and thanks for joining us!

@LSchermelleh @HeintzmannLab @beniroquai @StefanieReiche6 @RSocPublishing
Needless to mention, we strategically planned the 2-day #SIMposium to end today on #Twosday 😊
@royalsociety, @beniroquai @LSchermelleh - remember?😉

We had a lot of great talks, which might be of historical significance!


• • •

Missing some Tweet in this thread? You can try to force a refresh

Keep Current with Kirti Prakash🦉🐒

Kirti Prakash🦉🐒 Profile picture

Stay in touch and get notified when new unrolls are available from this author!

Read all threads

This Thread may be Removed Anytime!


Twitter may remove this content at anytime! Save it as PDF for later use!

Try unrolling a thread yourself!

how to unroll video
  1. Follow @ThreadReaderApp to mention us!

  2. From a Twitter thread mention us with a keyword "unroll"
@threadreaderapp unroll

Practice here first or read more on our help page!

More from @kirtiprakash25

Aug 11, 2021
Excited to share our new preprint on "Assessment of 3D MINFLUX data for quantitative structural biology in cells"
with @AlistairCurd


We thank @LSchermelleh @HohlbeinLab @michellepeckham for the feedback and looking forwards to yours!

Thanks, @Stefan_W_Hell for sharing the data which helped to revisit the claims made in the following article "MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells" @naturemethods

Hope our findings are useful for MINFLUX research!
First, using PERPL, our localization precision estimates of 0.98±0.02 nm, 3.20±0.05 nm and 3.31±0.08 nm broadly agreed with the published analysis but implying FWHM of 2.4-7.1 nm.
So, what should be the best theoretical resolution? 1-3 nm as claimed?
Read 17 tweets
Dec 9, 2020
Hey #STED, #MINFLUX people,
To continue the public discussion, I list here some of the points I made during my talk.

Summary: MINFLUX is a promising technology but at an early stage of development esp for biological research.


cc @Stefan_W_Hell @BalzarottiFran
Disclaimer 1: I haven't seen or worked with #MINFLUX setup, my arguments mainly on application-side

Disclaimer 2: I asked these questions via email (Sept 2020) and requested raw data and codes but haven't received a reply yet

I hope @rita_strack @naturemethods can help! Image
You can learn more about #MINFLUX from this excellent talk by @Stefan_W_Hell

Also, some papers making an effort in this direction ImageImage
Read 43 tweets
Dec 22, 2018
Today is 131st birthday of #Ramanujan, celebrated as #NationalMathematicsDay in India.
This year is also the 100th anniversary of his election as FRS.
131 is a palindromic and permutable prime with 113 and 311.
Can also be stated as the sum of 3 consecutive primes 41 + 43 + 47. Image
#Ramanujan's letter to Hardy is an all-time classic -
"I beg to introduce myself to you as a clerk"
"I have no University education"
"I have not trodden through the conventional ... but I am striking out a new path"
"I have made a special investigation of divergent series" Image
There are ~ 4 photos #Ramanujan according to
"Ramanujan: Essays and Surveys" by Bruce Berndt and co.

Ramanujan's Lost Notebook series here:
springer.com/series/7261 ImageImageImageImage
Read 14 tweets

Did Thread Reader help you today?

Support us! We are indie developers!

This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3/month or $30/year) and get exclusive features!

Become Premium

Don't want to be a Premium member but still want to support us?

Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal

Or Donate anonymously using crypto!


0xfe58350B80634f60Fa6Dc149a72b4DFbc17D341E copy


3ATGMxNzCUFzxpMCHL5sWSt4DVtS8UqXpi copy

Thank you for your support!

Follow Us on Twitter!