Thread by @ChrisMasterjohn

UPDATE:

Most of the redactions in the paper I posted appear to be two proprietary formulations that were not representative of the vaccine, whereas the modRNA-LNP8 data is representative of the vaccine and not redacted.

HOWEVER...

Page 19 implies that all modRNA-LnP8 was tested for bioluminescence at all time points, yet it seems everything but the 6h time point is redacted. So is the buffer control.

Page 20 blanks out the quantitative signal over time, but shows the area under the curve, for modRNA-LNP8.

Conceivably they could claim that the lines were crossing the proprietary lines on the left, but that could so easily be fixed, so it seems they are hiding the time course.

In fact hiding the timecourse is easier to reconcile to the redactions on the previous page, since the pictures of the mice under bioluminescence would obviously not intersect with one another.

So they seem to be hiding the persistence of the bioluminescence over time.

This is strange also because it is said to reach background levels by day 9, yet it obviously didn't descend all the way to the buffer since you can see open space above the buffer time point on day 9.

On page 21, it does show the modRNA-LNP bioluminescence of the mice facing stomach up, which I suppose shows the liver on the left, but it only goes 48 hours.

Notably, the mice were imaged stomach-up and stomach-down. So they seem to be hiding the stomach-down images (?) and the time points after 48 hours.

Further, where they do show the mice bioluminescing through 48 hours, they redact the entire panel quantifying this on the right.

This graph on page 22 is a good example where the redaction looks worse than it is.

It appears the redactions are entirely the two proprietary preparations unrelated to the vaccine while the control is shown on the left and the vaccine-related modRNA-LNP8 on the right.

That seems to be the case here as well.

And the same here.

In contrast to the cynical interpretation of the timecourse redaction, the text gives actual numbers for initial, 72h, and day 9 total bioluminescence.

Overall I believe I should retract my initial characterization of obviously hiding something.

I remain concerned why Figure 1A seems to have all but one of the vaccine-related images redacted.

I am concerned that they may be hiding bioluminescence in the ovaries.

We know that the images included both dorsal (on stomach) and ventral (stomach up).

Nothing in the caption of Figure 1A indicates whether the redacted images were dorsal or ventral or both.

I am not an expert in mouse anatomy, but @VikiLovesFACS had told me the last time we had leaked Pfizer bioluminescence data that the mice were on their stomachs (dorsal image) and the ovaries would be visualizable from their surface.

https://twitter.com/VikiLovesFACS/status/1409225300693688321?s=20&t=mcx2eoMKu0OSYur9rCdzig

It is unclear to me if this previously leaked Pfizer data is the same exact mouse study, but it showed dorsal images up to 6 days.

dropbox.com/s/v2im834kdp95…

If you look at my discussion with @VikiLovesFACS, one of my criticisms of her take that you could not see the luminescence in the ovaries in those pictures was that you can set the imager to different settings that can make it show up or disappear.

Thus all that we could conclude was that the ovary signal, if it existed, was much lower than that in the liver, since the liver was visible in the settings used in that picture.

It is clear that the pictures in Figure 1A, even if this is the same experiment, are different, because they include a 9 day time point and those previous pictures only went to day 6.

This document also has some ventral images.

So these are definitely different pictures, possibly from the same experiment.

My concern is that they could show days-long bioluminescence in something other than the liver, such as the ovary.

Previously, we had reason to be concerned that the lipids were increasing their proportion in the ovaries, adrenals, and bone marrow up through 48 hours, the longest time point measured.

dropbox.com/s/v2im834kdp95…

This data seems to be replicated in a different, much less redacted paper, from the current data dump:

phmpt.org/wp-content/upl…

Spot checking a few numbers, this seems to be the same data, but carried to more significant figures in the decimal.

As evidenced in the two threads pasted below, my concern was ALWAYS that the lipid data suggested the ovaries, adrenals, and bone marrow could be accumulating RNA well beyond the 48-hour mark and this was not studied.

It is deeply concerning, then, that the redacted images in Figure 1A could show evidence of bioluminescence up to day 9 in any of these other organs.

https://twitter.com/ChrisMasterjohn/status/1409267376386945031

https://twitter.com/ChrisMasterjohn/status/1408299859321528320