Thread by @TheMini2P

Hello, I am #Mini2P, a #twophoton miniscope brain explorer. I am #OpenSource. My team is headquartered at @KISNeuro in Trondheim.
@WikiZong @HorstObenhaus @EmilieSkytoen
@HannaEneqvist @NienkeLdeJong @MarinaRJorge @MayBrittMoser @EdvardMoser

👉 ntnu.edu/kavli/mini2P

https://twitter.com/KISNeuro/status/1504835085299851267

🤖💞🧠Watch the video about #OpenSource #Mini2P by @KISNeuro @NTNUnorway
norwegianscitechnews.com/2022/03/mini2p…

@KISNeuro @NTNUnorway #SciStory:
1/7 We provided 3 interchangeable objectives. Those objectives allowed us to monitor calcium activity in freely-moving mice targeting visual cortex, medial entorhinal cortex, & hippocampus. We were able to detect hundreds of neurons in a single FOV in all 3 regions.

2/7
A new Z-scanning device was developed. With four planes, we can record over 1,000 non-repeated cells in VC. Recordings in one mouse showed single-cell-resolution 3D functional imaging within a 420×420×160 μm3 volume, at a volume rate of 2 Hz
@KISNeuro

3/7
A FOV-stitching method was developed. We were able to extract 10,096 non-repeated layer 2/3 excitatory neurons across 5×5 FOVs in two planes. After correction of scan field distortions, all FOVs could be aligned to each other.
@KISNeuro

4/7
Hundreds of grid cells were recorded from two planes in medial entorhinal cortex.
@KISNeuro

5/7
We showed that adjacent grid cells in medial entorhinal cortex could have quite different phases!
@KISNeuro

6/7
We achieved stable MEC imaging in a 3D exploration task where a mouse was trained to climb vertically on a ladder to the top of a tall platform and then jump down before starting another climb.
@KISNeuro

7/7
We finally validated MINI2P performance in VC in an escape-to-shelter assay, using an elevated 90 cm diameter circular arena with an over-ground shelter placed close to the periphery.
@KISNeuro @WikiZong