We’re excited to share a greatly anticipated resource for microscopists. This protocol spills all the secrets behind our 8 color imaging in an easy to follow guide. Out today in @NatureProtocols ! #Imaging #Science #Organoid #Cancer @IamAnneRios @prinsesmaximac @oncodeinstitute
Read all about it and share with your colleagues and collaborators! nature.com/articles/s4159…
Our protocol explains in great detail how to do 3D multispectral imaging with online fingerprinting using the @zeiss_micro spectral detector. We guide you through mix design, sample preparation and we showcase applications such as single-cell analysis. 
In addition, we present an equalization assay which is instrumental to designing a functional 8-marker combination for multispectral imaging. An automated script for the analysis is also included!
mLSR-3D is now compatible with FFPE tissue, so it is possible to visualize your stored pathology samples in 3D with 8 colors.
mLSR-3D provides excellent preservation of tissue structure with high signal quality allowing large-scale 3D imaging of intact tissues, including small and large organoids.
We also included clever pre-processing tools from STAPL-3D to correct technical artefacts common to 3D imaging and a convenient shading correction tool that does not require pre-recorded shading references.
Curious about what else can be done with STAPL-3D? Go check our recent paper here:
https://twitter.com/Dream3DLab/status/1400476711767134215
The code and demos for STAPL-3D can be found on our Github: github.com/RiosGroup/STAP…
Now it’s time to grab this protocol and do some amazing 3D imaging! Thanks to everyone involved!
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