1/6 PGE2 is one of the principal bioactive prostaglandins, critical signaling lipids implicated in body homeostais and pathegenic inflammatory response, yet its in vivo dynamics are largely unknown. #GRABsensors #Prostaglandins
2/6 Now we newly developed a fantastic GRAB-PGE2 sensor for monitoring PGE2 dynamics with high spatiotemporal resolution in vivo!
3/6 Using the newly developed GRAB-PGE2 sensor, we detected spatiotemporal PGE2 release during inflammation and seizures in the mouse dorsal cortex by mesoscopic imaging.
4/6 In the IL-1β induced inflammation model, the PGE2 level gradually increased after i.p. and there are some hotspots around blood vessels.
5/6 In the KA-induced seizure model, cortical-wide PGE2 levels are increased after i.p. and maintained at a high level for tens of minutes, which is quite different from Ca2+ signals.
6/6 We are delighted to share the PGE2 sensor (and more GRAB sensors) with the community and hope they can help deepen our understanding of PGE2 modulation.
• • •
Missing some Tweet in this thread? You can try to
force a refresh
