Thread by @VicidominiLab

#quantum is here! Why not collect #fluorescence #photon-by-photon in your laser-scanning #microscope? We developed the #BrightEyes_TTM, an #opensource data acquisition system able to do it!
nature.com/articles/s4146…
@IITalk @ERC_Research @RNA_iit @MSCActions #BrightEyes_ERC
(1/5)

Our time-tagging #DAQ module can collect single-photon signals from 25 independent channels and reference signals from 3 channels. We showed how this module combined with a #SPAD array allows us to perform #FLISM and #FLFS. (2/5)
#imagescanningmicroscopy #fluorescence #lifetime

We characterized the #BrightEyes_TTM. We can reach a single-shot precision of 30 ps, 4 ns dead-time, and an "infinite" range in all channels, with a total flux of up to 125 MHz. (3/5)
#TCSPC #antibunching #quantum #microscopy #FPGA
#coding #LIDAR

Fluorescence has never been so informative! Thanks to the synergy between the #BrightEyes_TTM and the #SPAD array detector, we can perform #FLFS on living cells to simultaneously follow different dynamics parameters and fluorescence lifetime. (4/5)
#singlephotonmicroscopy

Want to have your own? Your Secret Santa has a gift for you! The #BrightEyes-TTM is available online as a #openscience project:
brighteyes-ttm.readthedocs.io/en/latest/
If you are interested in our projects DM us. We have #openposition.
#OpenScience #singlemolecule #superresolution