1/ If someone tells you that you can learn computation overnight, it is a lie. What I wish I had known 10 years ago with resource links👇🧵 #rstats #computationalbiology
2/ Spend time learning Linux commands. Fancy tools can become obsolete tomorrow, and Linux skills persist.
I started with this book linuxcommand.org/tlcl.php #unix
3/ Learn a language and learn it well. It does not matter if you pick R or python. If you can use it to solve your practical problems in the lab, that will be good. There is so much data wrangling to do, start with r4ds.had.co.nz #rstats
4/ Do not spend too much time choosing an IDE, you want something simple to get started. In my early days, I even tried Emacs speaks statistics which sucked my time to configure. If you use R, use Rstudio. If you use python, use spyder or pycharm. #rstats #python
5/ Exploratory data analysis is so important. make visualizations to check the data distribution, pattern, PCA to check sample swaps etc. arxiv.org/abs/2108.05182

read clauswilke.com/dataviz/
6/ Teach what you learned to someone else. This is the best way for your to understand something well. I taught my first carpentry workshop only 2 years after I started to learn R and UNIX divingintogeneticsandgenomics.rbind.io/talk/2015-miam…

Join it as an instructor carpentries.org @thecarpentries
7/ take training courses. I attended ANGUS NGS course by @ctitusbrown in 2014 and went back as an instructor a few years later. Highly recommend it ivory.idyll.org/dibsi/ANGUS.ht…
8/ I also attended Summer Institute in Statistics for Big Data (SISBID) si.biostat.washington.edu/about/sisbid
9/ spend one hour each day to study online with edx, cousera or audacity.
Two courses I recommend:
edx.org/professional-c…

edx.org/professional-c…
10/ Learn by doing. Take a project in which you can use your skill sets. With determination and discipline, you can learn anything! yeah, it takes time, but you should take action NOW!
That's a wrap!

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More from @tangming2005

Jan 10
1/ What people think bioinformatician do:
credit: @torstenseemann
2/

what I really do 👇

google "ylim without removing data in ggplot" to find coord_cartesian(ylim=c(0, 7)) #rstats every time!
3/ This StackOverflow post has over 1 million views and I contributed many times

by googling: how to rotate x-axis label

stackoverflow.com/questions/1330…
Read 5 tweets
Jan 9
1/
During my years in the lab, I observed that some people hoard their scripts and do not want to share or teach others.

I was the opposite. Although I was a beginner, I taught all that I knew and share my scripts with other lab members who were beginners too.
2/ The results? I learned so much by teaching!
If you want to learn something better, teach it to others.

I can not believe how much I learned by writing my own book too!
3/ Remember to acknowledge people who have shared scripts with you. They deserve the credit.
Read 4 tweets
Dec 14, 2022
15 tools/papers for multi-sample multi-group single-cell RNAseq differential expression analysis 🧵👇
compiled at crazyhottommy.blogspot.com/2022/12/15-too…
1/ [An Empirical Bayes Method for Differential Expression Analysis of Single Cells with Deep Generative Models](biorxiv.org/content/10.110…) scVI-DE
Read 17 tweets
Nov 30, 2022
Making a heatmap is an essential skill for a bioinformatician.
But you probably do not understand heatmap. 7 reading resources to understand heatmap!👇🧵
compiled at crazyhottommy.blogspot.com/2022/11/7-link…
1/ Mapping quantitative data to color nature.com/articles/nmeth…
2/ Heat map from Nature Method column nature.com/articles/nmeth…
Read 10 tweets
Nov 29, 2022
6 training resources for data management 👇🧵

compiled at crazyhottommy.blogspot.com/2022/11/6-trai…
1/ Best Practices for Biomedical Research Data Management learn.canvas.net/courses/1854
2/ Research Data Management Librarian Academy (rdmla.github.io)
Read 9 tweets
Nov 28, 2022
10 command-line and R tools to deal with excel, tsv, csv files👇🧵
compiled at here too crazyhottommy.blogspot.com/2022/11/8-rcom…
1/ [readxl](readxl.tidyverse.org) to read excel file
2/ [tidyxl](github.com/nacnudus/tidyxl) to tidy excel file
Read 10 tweets

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