This is a dense read but it will help you to understand why the DNA fluorometer data is lighting up so much on modRNA. This is much more cross-talk than you see with traditional ssRNA.
That is because these are intercalating dyes that bind to minor grooves are only found in double stranded nucleic acids.

The fact that RNaseA is showing such a reduction in signal is because the RNA isnt single stranded.
At least half of it is digestable by a dsRNA specific nuclease known as RNaseIII.
Another portion responds to RNaseH which only digests RNA in RNA:DNA hybrids.

The fraction that responds to RNAseIII should worry you. Your cells have RNaseIII and its the start of the RNA interference pathway.
To make matters worse, Pharma is using an ELISA that is blind to dsRNA <400bp. It cant see this problem. And they know it exists

Read 7 tweets

Kevin McKernan

@Kevin_McKernan

Dec 3

This is worth a full read.
They really knocked it out of the park on this study.

publichealthpolicyjournal.com/biontech-rna-b…

First…
They measure how long is spike expressed in cells.
7 days out it is higher than day 1. Peaks at day 5.

Using 3 different Fluorometry methods they zero in on DNA levels before and after RNaseA treatments.
4-5X over once RNA is removed.
The AccuBlue dyes had the least cross talk.

Read 10 tweets

Kevin McKernan

@Kevin_McKernan

Nov 3

If there is one paper you should read this weekend…

This is it.

It’s not too thick.

journals.asm.org/doi/pdf/10.112…

I’ve posted on this before and have a stack about this if you want a TL/DR

They demonstrate research plasmids used to study the vaccine, infect lab staff and their housemates.

This triggered a fake casedemic as they all tested positive for Nucleocapsid on COVID tests.

What if this plasmid encoded something more inflammatory like spike?

These are shuttle vectors that can replicate in ecoli and mammalian cells.
They can leave the lab with employees and infect others.

Read 7 tweets

Kevin McKernan

@Kevin_McKernan

Oct 18

Your response to peoples request for your qPCR protocol is misinformation.
No transparency on your methods but we can already see that you are using only 1 assay in the vector (Kan gene) so you are under estimating the load 100X.

You should know this by now as Speicher et al published this a year ago.

@TGAgovau This is misinformation. Dr. David Speicher's latest test used RNaseA. There is no RNA interfering with the fluorometry.
Did you not even read the report?
Life must be good at the TGA when you can be a year behind all the time.

@TGAgovau Part of GMP and ISO is having your protocol open for inspection.

Rules for thee but not for me at the TGA.

Read 4 tweets

Kevin McKernan

@Kevin_McKernan

Oct 14

Is SV40 Large Tumor Antigen required for SV40 origin of replication activity.

No

Here we detect Pfizer plasmid DNA in a colon tumor biopsy 1 year after vaccination.

And its not small amounts of DNA. Its so much DNA that it can only be explained by plasmid amplification post vaccination or genome integration and amplification.

Variants are found in the SV40 Promoter that do not exist when you sequence the vaccine directly suggestive of replication errors once transfected into mammalian cells.

Read 8 tweets

Share this page!

Enter URL or ID to Unroll

Kevin McKernan

Try unrolling a thread yourself!

More from @Kevin_McKernan

Kevin McKernan

Kevin McKernan

Kevin McKernan

Kevin McKernan

Kevin McKernan

Kevin McKernan

Did Thread Reader help you today?

Don't want to be a Premium member but still want to support us?

Send Email!