Your response to peoples request for your qPCR protocol is misinformation.
No transparency on your methods but we can already see that you are using only 1 assay in the vector (Kan gene) so you are under estimating the load 100X.
You should know this by now as Speicher et al published this a year ago.
@TGAgovau This is misinformation. Dr. David Speicher's latest test used RNaseA. There is no RNA interfering with the fluorometry.
Did you not even read the report?
Life must be good at the TGA when you can be a year behind all the time.
@TGAgovau Part of GMP and ISO is having your protocol open for inspection.
Is SV40 Large Tumor Antigen required for SV40 origin of replication activity.
No
Here we detect Pfizer plasmid DNA in a colon tumor biopsy 1 year after vaccination.
And its not small amounts of DNA. Its so much DNA that it can only be explained by plasmid amplification post vaccination or genome integration and amplification.
Variants are found in the SV40 Promoter that do not exist when you sequence the vaccine directly suggestive of replication errors once transfected into mammalian cells.