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Kevin McKernan

@Kevin_McKernan

Mar 30

H/T Dr. David Wiseman.

fda.gov/vaccines-blood…

Note the limit set by VRBPAC.
Note the differentiation of DNA types.
Protooncogenes…
Where does an SV40 promoter with a nuclear localization signal fit?

@BretWeinstein

Is C57BL6 the right model for this?
These lab mice have extended telomeres and are less prone to cancer.
@BretWeinstein

Read 4 tweets

Kevin McKernan

@Kevin_McKernan

Mar 25

I get this comment a lot.
They demand I jump through hoops while handing a hall pass to the manufacturers.

Yet its my friends and family that are faced with the threat of these injections if they want a job or an education.

The demand for peer review is always rooted in a midwits distrust in the average intelligence of their peers.

They are afraid other people will think for themselves and can only trust a clergy class to declare the truth we must all listen to.

Modern peer review doesn't guarantee reproducibility. Science is about the latter not the former.

When you lose sight of this, attack surfaces emerge.
Centralization of peer review by journals funded by Pharma who then demand scientists perform free work? Hows that working out?

Read 4 tweets

Kevin McKernan

@Kevin_McKernan

Mar 19

https://twitter.com/rt7683/status/1637523324619169792

It’s been 3 years since Nobel laureate Andrew Fire published the sequence of the monovalent vaccines in GitHub (not peer reviewed). They didn’t put reads public.

Where was the outrage over their lack of peer review?
Lack of raw sequence data?
Lack of qPCR, UV spec, etc?

https://twitter.com/rt7683/status/1637523324619169792

The only time we see outrage is when a group does it with 9 orthogonal techniques and nails the fact that the vectors are there.
RNA-seq
qPCR
RT-qPCR
UV Spec
Quibit
Agilent TS
RNAse seq
DNase/ RNase qPCR.
Ecoli transformation

Our seq is public. Our primers are public.

The low wattage selective outrage is overt. It has nothing to do with quality and everything to do with ideology.

Where was their outrage over Andrew Fires GitHub release of the vaccine sequence with no raw reads to back up their claims?

github.com/NAalytics/Asse…

Read 6 tweets

Kevin McKernan

@Kevin_McKernan

Mar 16

The SV40 promoter is in front of the Kanamycin resistance gene.

This provides both bacterial and mammalian expression of the Kan Gene.
Famous paper from Southern and Berg
pubmed.ncbi.nlm.nih.gov/6286831/

sciencedirect.com/science/articl…

Read 5 tweets

Kevin McKernan

@Kevin_McKernan

Mar 15

Antipodes has a very perceptive question.
You can’t fight a centralized Fiat public health hierarchy with their data.

They will keep hiding the ball on you.

You must take a decentralized approach.

Public health data has been manipulated. These are mutable databases that go offline strategically.

We must focus on aspects of the tech that anyone around the world can verify.

The genomic impurities of the vaccine can be measured with simple PCR tools and defy regulations.

These machines have spread wide and far measuring C19 and are going idle.

They need to be fed and billions of people are starting to ask what the hell have they done.

They can’t jockey a PH database and dent this problem. For many it’s pushing on a rope.

Read 5 tweets

Kevin McKernan

@Kevin_McKernan

Mar 14

pUC around and Pfind out-
The EMA document claims Pfizer linearizes their plasmids with Eam1104i. This is a restriction enzyme that cuts DNA based on the below recognition sequence.

Lets looks through the Deep Sequencing in IGV and see if we can find intact molecules.

Upper left is an obvious place where the Cuts end the molecules we sequence.
Upper right is another group of reads where the cut sites are intact.

Lets zoom in on that so you can see the details.

Sequencing OGs will notice there is more sequencing error near these sites as we near poly As and some sequencing adaptors. What does that mean?

Read 13 tweets

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