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Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Jun 13, 2023 • 39 tweets • 20 min read • Read on X
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1. OK, the Lab leak CLUEDO game is nearing its finale

Ben Hu, Yu Ping, and Yan Zhu now identified (likely by an ex-State Department source) as the 3 sick WIV researchers in November 19.

public.substack.com/p/first-people… Image
2. We researched these particular scientists

(as we did with all WIV staff in 2020 and 2021)

3. I think everyone is familiar with Ben Hu and his work

(involving bat coronaviruses and hACE2 mice)

4. Ben Hu

There were indeed persistent and informed rumors that Ben Hu was one of the sick researchers, but unfortunately #DRASTIC was unable to access Wuhan Health Records, despite my best attempts & appeals for assistance in May 2020

5. A Million Apologies!

Unfortunately I lacked the funding necessary to pursue this undiscover work to its full extent.

I remember cursing that we did not have the meager resources of even a third world intelligence agency to support our investigations.

6. This would not have been the first time...

Back in 2017 we showed that WIV bat samplers had been scratched, bitten and infected while sampling without proper PPE

7. The 2nd named researcher, Yu Ping:

Yu Ping's PhD thesis (discovered by @TheSeeker268) describes working with Ra4991 (RaTG13) *other samples in a 'BSL-2 cabinet' via @wound_wort

In fact the whole genome sequencing for Ra4991 (RaTG13) was reported by Yu Ping in June 2019 ImageImageImageImage
8. More on Yu Ping's thesis

9. Yu Ping and Ben Hu and ZLS (2018-2019)

Geographical structure of bat SARS-related coronaviruses

sciencedirect.com/science/articl…

or

ncbi.nlm.nih.gov/pmc/articles/P… Image
10. Yu Ping seems to have "faded into obscurity"

11. Meanwhile, the 3rd identified researcher, Zhu Yan:

"Senior Experimentalist" at WIV has been listed as co-author on so many WIV papers.

archive.is/g9tt9 Image
12. Yan Zhu vs Yu Ping

Yan Zhu is listed as co -author with Zhengli Shi on 13 papers here:

whiov.cas.cn/sourcedb_whiov…

Search: "Zhu, Y."

but Yu Ping, Zero.... Image
13. Yan Zhu Research Profile

researchgate.net/scientific-con…
14. Beginning in 2013 (Yan Zhu, Ben Hu, etc.)

Recognize some names?

Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor

nature.com/articles/natur… Image
15. 2017 (Yan Zhu & Zheng Li Shi)

Detection and genome characterization of four novel bat hepadnaviruses and a hepevirus in China

ncbi.nlm.nih.gov/pmc/articles/P… Image
16. 2018 (Yan Zhu & Zhengli Shi)

Genomic Characterization of a Novel Hepatovirus from Great Roundleaf Bats in China

link.springer.com/article/10.100… Image
17. March 2018

(Yan Zhu, Lin-Fa Wang, Zheng-Li Shi, Peng Zhou)

Dampened STING-Dependent Interferon Activation in Bats

ncbi.nlm.nih.gov/pmc/articles/P… Image
18. April 2018

(Yan Zhu, Peng Zhou, Daneille Anderson, Peter Daszak, Linfa Wang, ZLS, Yi Gang Tong (GX Pango Man)

Fatal swine acute diarrhoea syndrome caused by an HKU2-related coronavirus of bat origin

nature.com/articles/s4158… Image
19. October 2018

(Yan Zhu, ZLS, Ali Zohaib)

Countrywide Survey for MERS-Coronavirus Antibodies in Dromedaries and Humans in Pakistan

ncbi.nlm.nih.gov/pmc/articles/P… Image
20. December 2018

(Yan Zhu, Ali Zohaib, Ben Hu, Peng Zhou, Xing Yi Ge, ZLS)

Genetic Evidence of Middle East Respiratory Syndrome Coronavirus (MERS-Cov) and Widespread Seroprevalence among Camels in Kenya

ncbi.nlm.nih.gov/pmc/articles/P… Image
21. March 2019

(Yan Zhu, Danielle Anderson, Peng Zhou, Lin Fa Wang, ZLS)

Characterization of a filovirus (Měnglà virus) from Rousettus bats in China

nature.com/articles/s4156… Image
22. April 2019.

( Yan Zhu, ZLS, Ben Hu)

Characterization of a New Member of Alphacoronavirus with Unique Genomic Features in Rhinolophus Bats

ncbi.nlm.nih.gov/pmc/articles/P… Image
23. October 2019.

(Yan Zhu, Ben Hu, Danielle Anderson, Lin Fa Wang, Peng Zhou, ZLS)

Serological evidence of MERS-CoV and HKU8-related CoV co-infection in Kenyan camels

ncbi.nlm.nih.gov/pmc/articles/P… Image
Then the Pandemic, and just afterwards..

30th January 2020

(Yan Zhu, Peng Zhou, ZLS)

SARS coronavirus 2 WIV06 Project

ngdc.cncb.ac.cn/gwh/Assembly/9…

and the paper (usual suspects)

pneumonia outbreak associated with a new coronavirus of probable bat origin

nature.com/articles/s4158… ImageImageImage
25. January 29th 2020

(Yan Zhu, Danielle Anderson, Zheng Li Shi, Lin Fa Wang, Peng Zhou)

Discovery of Bat Coronaviruses through Surveillance and Probe Capture-Based Next-Generation Sequencing

ncbi.nlm.nih.gov/pmc/articles/P… Image
26. May 2020

(Yan Zhu et al.)

(The mysterious transgenic mice paper received on 12th March 2020, during lockdown, so mice must have been available in 2019)

Pathogenesis of SARS-CoV-2 in Transgenic Mice Expressing Human Angiotensin-Converting Enzyme 2

pubmed.ncbi.nlm.nih.gov/32516571/ Image
27. For more on that curious paper, see here:

28. December 2020

(Yan Zhu with WIV and WIBP)

Low toxicity and high immunogenicity of an inactivated vaccine candidate against COVID-19 in different animal models

ncbi.nlm.nih.gov/pmc/articles/P… Image
29. January 2021 - The most Important paper!

(Yan Zhu, Ben Hu, ZLS, Xing Yi Ge)

Characterization of Novel Rhabdoviruses in Chinese Bats

ncbi.nlm.nih.gov/pmc/articles/P… Image
30. Why the most Important, you may ask?

Well you had to wait for the cracker, didn't you?

after wading through tons of Bloody Chinese Academic BAT SHITE, like I had to do for 3 bloody years!!!



Now, f@##!@#$$% READ that thread!
31. And read this as well

32. May 2021 Pre Print

(Yan Zhu, Ben Hu, ZLS)

This study identified a novel lineage of SARSr-CoVs, including RaTG15 & 7 other viruses, from bats at the same location where we found RaTG13 in 2015.

biorxiv.org/content/10.110…

Later published in August 2021

ncbi.nlm.nih.gov/pmc/articles/P… Image
33. March 2023

(Yan Zhu, Ben Hu, ZLS)

Isolation of ACE2-dependent and -independent sarbecoviruses from Chinese horseshoe bats

biorxiv.org/content/10.110… Image
34. Increasingly desperate Zoonati clutching at straws

brandish a well known WIV happy family photo with Ben Hu, ZLs & LFW from 15 Jan 2020 as PROOF that he could not have been infected in November 2019?

as if everyone who got infected, died or what?

35. Their Final Argument?

"But, but, Professor Shi said they tested everyone and all the results were negative..."

For example:

The origins of SARS-CoV-2: A critical review

sciencedirect.com/science/articl… ImageImage
36. That Argument was Solidly Debunked

by @quay_dr and @gdemaneuf

An analysis of the results of routine employee testing for SARS-like infections within the WIV and other Wuhan labs raises serious issues about their validity

researchgate.net/publication/35…
37. Professor Shi's Denial

A Top Virologist in China, at Center of a Pandemic Storm, Speaks Out

The virologist, Shi Zhengli, said in a rare interview that speculation about her lab in Wuhan was baseless

archive.ph/LfZN1 Image
unroll 3 sick researchers at WIV @threadreaderapp Image
save @rattibha

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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Aug 22
1. Nord Stream Update

3 years for truth to emerge

@MerlynThomas on 27/09/22 for @BBCWorld

Ukraine accuses Russia of pipeline terror attack

bbc.com/news/world-eur…

@JamesCrisp6 on 28/09/22 for @Telegraph

Why Putin would want to blow up Nord Stream 2

archive.ph/JCtYB x.com/Panchenko_X/st…
2. Hilariously

Merlyn Thomas has been working for the "BBC disinformation unit" since September 2021.

He certainly earned his UK STATE propaganda pay check with Nord Stream!

3. Invitation to venture down a Baltic rabbit hole!

You are hereby invited to read my 682 tweet thread investigating all angles of the Nord Stream sabotage, which ends here:



Thanks @Erkperk @VasilijProzorov @JeffreyBrodsky5 @MichaKobs @bopanc @liscer72
Read 4 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Aug 13
Baric's hACE2 transgenic mice were shared with the WIV for GOF like virus experiments designed to "assess" the potential for coronaviruses to jump from animals to humans.

We asked how many were sent to WIV

researchgate.net/publication/35…

Record via @jhas5 here

arlo.riseforanimals.org/entity/univers… x.com/jhas5/status/1…
2. Baric spills his humanized mice beans to Father Racaniello during a heart rending TWIV confessional in March 2020, admitting he sent his hACE2 mice to WIV in 2018 (as part of the infamous NIAID grant mentioned above):



Timestamp; 01:00:30 - 01:03:15microbe.tv/twiv/twiv-591/
3. Daszak

"Our R01 work shows that bats in South China harbor many SARSr-CoVs (50+), some of which are able to use human ACE2 to enter human cells, infecting humanized mouse models to cause SARS-like illness, and evade available therapies or vaccines"

ecohealthalliance.org/wp-content/upl…
Read 9 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Aug 11
1. New England Biolabs & Novagen

The amplified Rp3-S1 fragment was digested with BamHI & XhoI & ligated into the expression vector pMAL-C2X (New England Biolabs).

The 5 truncated fragments were digested with BamHI & XhoI and ligated into the expression vector pET32a (Novagen).
2. Promega & Takara

Protein expression & purification

After purification, recombinant plasmid was transformed into BL21 competent cells (purchased from Promega)

Recovered PCR product & expression vector pET32a were digested with restriction endonucleases BamHI & XhoI (Takara).
3. Takara (2)

After DNA recovery, the PCR product was ligated to the expression vector pET32a using T4 DNA ligase (Takara)
Read 32 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 26
@breakfast_dogs @gadboit @NLink247 1. Insect cell baculovirus cell cultures become contaminated with insect genomic material.
2. Replication process can lead to presence of viral DNA & cellular components in culture medium.
3. This LEADS to contamination of viral product with baculovirus & insect cell-derived DNA.
@breakfast_dogs @gadboit @NLink247 Contamination

4. Expression of SC-2 Spike RBD Using the Insect Cell-Baculovirus System

pmc.ncbi.nlm.nih.gov/articles/PMC97…

5. Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture

pmc.ncbi.nlm.nih.gov/articles/PMC71…

6. insect cell lines
sciencedirect.com/science/articl…
@breakfast_dogs @gadboit @NLink247 Did WIV use insect cell systems with SARS-C0V-2?

7. Insect cell baculovirus expression systems

x.com/BillyBostickso…

8. DENG Fei, Chief Investigator in Research Group on Viruses Resources at WIV engineered baculovirus-insect cell expression systems.

buzzchronicles.com/b/all/17652/
Read 13 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 22
1. Kadlec awaiting confirmation for his new role

2. Kadlec's report references pangolins 76 times

bush.tamu.edu/wp-content/upl…

3. Time to read my pangolin coronavirus report?

researchgate.net/publication/39…

4. DRASTIC research cited
@gdemaneuf @ydeigin @BillyBostickson @C_Small_ x.com/BoDeepest/stat…
1. the article linked is quite good, more comments and analysis will be posted here soon

city-journal.org/article/robert…Image
2. Possible lab manipulations

"2) insertion of a pangolin virus recombinant spike or the spike’s RBD protein that includes a sequence for an FCS and an integrin-binding protein" Image
Read 76 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 9
Pressure is needed on people like @realannapaulina @RepLuna & @GOPoversight team as their mandate may dissolve in August.

They did Kennedy, but they are supposed to do UFO files & CoVid-19 origins before they dissolve.

We also need to know about the subpoenas sent by @RandPaul
What was promised and demanded (for the record)

oversight.house.gov/release/rep-lu…

oversight.house.gov/release/chairm…
If you are a concerned US citizen please directly contact the people listed here:

oversight.house.gov/subcommittee/t…
Read 8 tweets

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