SV40 origin is only active in people who have SV40 T-Antigen expressed. Many people (100M) are SV40 infected suggesting they may be present in these individuals
There is an SV40 poly A signal downstream of the F1 origin but this is omits many of the other SV40 components in expression plasmid.
What is the F1 origin?
This can be used to make single stranded copies of the plasmid DNA for viral packaging in mammalian cells or bacterial strains.
The direction of the yellow arrow directs the + strand of synthesis.
The above paper is where much of the cancer risk of SV40 comes from. This relates to people infected with the full 5,234 viral genome, not just the components in the expression plasmid. What happens with SV40 infected people are vaccinated?
Patents are not the only way to secure business interests. Trademarks, trade secrets and licensing deals can be just as potent.
Corbevax TM has a trademark and many licensing deals are in place to deliver this cheap vaccine. It is cheap and 'likely' a safer approach than mRNA
There is a difference in saying SV40 promoters are not carcinogenic because they are missing the T-antigen …
And saying
We don’t know what will happen regarding genome integration.. or what will happen to the substantial part of the population already infected with SV40 virus.
A non trivial amount of the population already has SV40 virus capable of making the T antigen.
Injecting SV40 promoters and the SV40 origin into these patients may have unintended consequences.
Saying something doesn’t cause cancer and saying we don’t know are very different statements the fact checkers don’t like to face.
I go back to Keith Pedens work at the FDA looking at genome integration risks and oncogenesis.
I have had emails with JJ going back to December alerting him to what I saw as some of the holes in his Infectious clone hypothesis.
It’s not fair to say I sprung a dunk on this. I would get asked repeatedly to address it and finally got tired of piecemeal explaining the issues.
So I wrote everything into one thread that probably felt like a blind sided attack since he’s not on Twitter. But most of this I had already aired to him offline.
I also took his feedback on a Substack I wrote on the topic and edited things.
My experience with PubPeer has been very poor.
Wont let me register as my domain name at Medicinal genomics is not 'recognized'.
So if anyone else is registered and wants to point out this fake trace in a Drosten paper, please do.
There is no way...
The primers depicted in that extended data fig 1 generated those sanger traces as sanger doesnt sequence your primers. If a different set of primers were used to 'validate' the qPCR assay,
1)what were they?
2)using different primers to validate the fidelity of your PCR is fake
You are no longer measuring the fidelity of you PCR primers if you use a different one for sequencing.
The reverse primer can be read with Sanger if its close enough to the Forward primer (under 600bp). Must be on the 3' of the sanger trace for this to happen.
Oncologist’s employer takes Pfizer donations.
Claims it’s against his institutions policy to sequence his fresh vaccines.
Demands I send them our expired vials that he spent days chastising the veracity of.
David has exposed himself as having zero impartiality on the topic.
He has access to fresh vials but won’t touch those.
He wants our vials which he claimed were insufficient for publication and an embarrassment to work with.