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Adrian Sanborn Profile picture
Adrian Sanborn
@AdrianSanborn
Transcriptional regulation, 3D genome, high-throughput screening of protein function, machine learning, biophysical modeling. Kornberg Lab at Stanford.
Jan 3, 2023 • 10 tweets • 5 min read
Excited to share a new preprint! We do a pooled, imaged-based CRISPRi screen on the complex shapes and cytoskeletal structures of human U2OS cells:
biorxiv.org/content/10.110…
Co-led with R. Lorenzo Labitigan in Julie Theriot's lab, with special shoutout to Cynthia Hao! 1/10 Image Human osteosarcoma U2OS cells are a classic system for fine-grained study of the actin cytoskeleton. U2OS are motile and exhibit very wide variation in morphology & internal organization! Mechanisms governing this intercellular diversity are not well understood. 2/10
Dec 22, 2020 • 17 tweets • 9 min read
Highlights from our #preprint on #transcription activation domains (ADs)!

-found all ADs in yeast TFs
-predicted yeast & human ADs
-tested binding of all ADs to #Mediator & #TFIID
-explained mechanism with structural modeling & kinetic experiments

(1/16)
biorxiv.org/content/10.110… Image We tested ~7500 53aa tiles covering all 164 yeast transcription factors (TFs) in an activation screen in vivo.

By controlling for fragment expression & sorting GFP activation into 8 bins, we get quantitative measurements with super high signal-to-noise. We found 150 ADs. (2/16) Image