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Daniel Hurdiss Profile picture
Daniel Hurdiss
@DanielHurdiss
Assistant Professor @UtrechtVirology 🇳🇱 | Former MSCA & EMBO Fellow | PhD @AstburyCentre 🇬🇧 | #Virology, #CryoEM & #SciArt | Views are my own.
Jan 21, 2022 • 13 tweets • 6 min read
A thread on how we determined 12 sub-3 Å spike-Fab #cryoEM structures from a single microscopy session❗️ Using #EPUMultigrid, we performed high-throughput epitope mapping for a panel of neutralising antibodies targeting the #SARSCoV2 spike protein 🧵

thermofisher.box.com/s/a8mws76g5ew8… The inspiration for this project came from our previous observation that the 6P-stabilised #SARSCoV2 spike (@McLellan_Lab) could produce ~3 Å structures from less than 2 hours of data collection👇